Calcitonin cell population and distribution in the thyroid gland of the rat

Monsour, P. A.; Kruger, B. J.; Barnes, Andrew C.

doi:10.1002/jmor.1051860304

Cited by 14 publications

(24 citation statements)

References 6 publications

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“…Somatostatin has also been shown to be present in few cells in the thyroids of humans, dogs and rats (Yamada et al, 1977). The present results showed marked variability in the distribution of calcitonin-positive cells with age and the number appeared to increase with age, similar to the observations in rats (Monsour et al, 1985). Calcitonin was originally discovered as a hypocalcaemic factor in several mammals.…”

Section: Discussionsupporting

confidence: 75%

Histological and immunohistochemical changes of the thyroid gland during the foetal and postnatal period of development in indigenous Large White crossbred pigs

Igbokwe¹,

Ezeasor²

2015

BJVM

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The present study was carried out to ascertain the morphometric, histological and immunohistochemical changes (expression of calcitonin and somatostatin) in the thyroid gland of indigenous local pig crosses during the foetal and postnatal period of development. A total of thirty foetal thyroids (40) grouped into early and late foetal period, fifteeen (15) prepubertal and ten (10) pubertal thyroids obtained from slaughtered pigs in the local abattoir were used. Histomorphometrically, all the measured parameters increased with development in all thyroid age group. The mean values were significantly different at P˂0.05 among ages. Histologically, significant age-related variations in the structure of the foetal and postnatal thyroids were observed in the follicles, colloid, colloid vacuoles, follicular cells and interfollicular connective tissue. Calcitonin was demonstrated in the foetal and postnatal parafollicular cells, while somatostatin was only localised in the pubertal parafollicular cells. The study provides strong evidence of thyroid functionality during the late foetal period that changes with age during the development.

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Section: Discussionsupporting

confidence: 75%

Histological and immunohistochemical changes of the thyroid gland during the foetal and postnatal period of development in indigenous Large White crossbred pigs

Igbokwe¹,

Ezeasor²

2015

BJVM

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“…The appearance and distribution of C-cells found in our serially cut thyroid glands agreed well with those reported earlier where other fixatives and thinner tissue sections were used (Arias et al, 1989;Garel et al, 1981;Treillou-Lahille et al, 1981;Blahser, 1978;Petk6 et al, 1976;Baber, 1876). Quantitative studies of the C-cells in rats have been published earlier, but the quantifications were performed in 4-6 pm (2-D) sections with the aid of points, frames, or grids (Hwang et al, 1986;Monsour et al, 1985;Garel et al, 1981;De L e k et al, 1979;Peng et al, 1976), which causes various biases owing to the fact that the size, shape, and orientation of the particles counted (cell or nucleus) influence the results (Cruz-Orive and Weibel, 1990;Gundersen et al, 1988). It has been emphasized in stereology that the particles observed in a 2-D section are not a uniform sample but a biased, nonrepresentative sample of all particles.…”

Section: Numbers Offields (Zf) Used To Sample C-cells Actual Numbesupporting

confidence: 76%

“…In some of these studies, the two-dimensional numerical density of cell or nuclear profiles has been used as a measure of their long-time endocrine activity (Arias et al, 1989;Zabel et al, 1987;Hwang et al, 1986;Monsour et al, 1985;Garel et al, 1981). Counting has been performed in routinely processed tissue sections.…”

Section: Introductionmentioning

confidence: 99%

Estimation of the C-cell numbers in rat thyroid glands using the optical fractionator.

Feinstein

Westergren²,

Bucht³

et al. 1996

J Histochem Cytochem.

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We estimated the total number of calcitonin-immunoreactive C-cells in rat thyroid gland using the optical fractionator, the unbiased stereological method for estimation of numbers. It was necessary first to use a fiiative composed of formalin, acetic acid, and ethanol to distinctly visualize the C-cells. The 40-pm-thick sections had to adhere to chromalum-gelatin-coated Superfrost Plus glass slides, and the immunostaining technique had to stain the C-cells evenly throughout the whole sections. Because the C-cells were irregularly distributed in the thyroid tissues, their counting required screening of about 500 fields per lobe, but the number of C-cells counted need not be high, about 90 per lobe. We estimated that rats have 185,000 2 42,000 C-cells (mean SD; n = 7). The C-cell population did not differ signifi-

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“…To randomize selection, entire thyroids were cut starting at the superior border, every tenth section (5 µm thick) was put aside for staining [21].…”

Section: Methodsmentioning

confidence: 99%

Age-associated functional morphology of thyroid and its impact on the expression of vimentin, cytokeratins and VEGF. The role of nigella in refinement

Zaki

Mohamed

Fattah

et al. 2018

Folia Histochem Cytobiol.

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Introduction. Aging causes morphological and functional changes in the thyroid gland. Free radicals play a key role in the pathology of normal aging. Vimentin and cytokeratin are cytoskeletal intermediate filaments that are often used as indirect indices of tissue injury. The aim of the study was to clarify the age-related alterations in the structure and function of the thyroid gland. The relationship between oxidative/antioxidative stress markers and cytoskeletal intermediate filaments (vimentin and cytokeratin) and oxidative/antioxidative stress markers as well as vascular endothelial growth factor (VEGF) during aging were elucidated. Finally, the role of Nigella sativa (NS) oil in ameliorating age-related alterations of the structure and function of the thyroid gland was studied. Material and methods. Thirty Sprague-Dawley albino rats were divided into five groups: young adult control, young adult NS-treated, late adult control, late adult NS-treated, and senile. The age of young adult, late adult, and senile rats was nearly 7, 18 and 22 months, respectively. NS oil was added to food pellets and was administered at a daily dose of 0.1 g/kg body weight for one month. The thyroid gland was dissected and fixed immediately in 10% formalin saline. The assessment of thyroid structure was based on hematoxylin and eosin, and Masson's trichrome stainings, and histomorphometric analysis of the deparaffinized sections. Localization and distribution of vimentin and cytokeratin filaments was assessed by immunohistochemistry. Measurements of VEGF gene expression by qPCR and oxidative/antioxidative markers (malondialdehyde and glutathione content, superoxide dismutase activity) in thyroid gland homogenates were performed. Serum concentration of thyroid hormones (T3, T4) and TSH were assessed by radioimmunoassay. Results. Follicles in the late adult control group were dilated and disrupted. Follicular cells showed cytoplasmic vacuolation. Follicles in the thyroids of senile rats were of irregular shape, often with cellular exfoliations. Many follicles were dilated and lined with flattened cells. A notable amelioration of these morphological alterations was observed in late adult NS-treated rats. Decrease in serum T3 and T4 levels and increase in TSH levels were observed in the late adult control and senile groups. A clear shift of the oxidative/antioxidative markers (MDA/ /GSH, SOD) was observed in the late adult control and senile groups in favor of oxidants. Administration of NS to late adult rats resulted in normalization of these parameters. Increased area of collagen fibers, immunoreactivity of vimentin and cytokeratin filaments and VEGF gene expression were observed in the thyroids of late adult and senile rat groups as compared to young animals. The mean number of follicular cells decreased in the late adult control and senile groups. Administration of NS to the late adult rats returned these parameters to the level of the young adult rats.

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Calcitonin cell population and distribution in the thyroid gland of the rat

Cited by 14 publications

References 6 publications

Histological and immunohistochemical changes of the thyroid gland during the foetal and postnatal period of development in indigenous Large White crossbred pigs

Histological and immunohistochemical changes of the thyroid gland during the foetal and postnatal period of development in indigenous Large White crossbred pigs

Estimation of the C-cell numbers in rat thyroid glands using the optical fractionator.

Age-associated functional morphology of thyroid and its impact on the expression of vimentin, cytokeratins and VEGF. The role of nigella in refinement

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