Calcitonin: characterisation and expression in a teleost fish, Fugu rubripes

Clark, Melody S.; Bendell, L; Power, Deborah M.; Warner, S.; Elgar, Greg; Ingleton, P. M.

doi:10.1677/jme.0.0280111

Cited by 31 publications

(21 citation statements)

References 53 publications

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“…Family 2 of G-protein couple receptors between vertebrates and invertebrates (Cardoso et al, 2006) and even then with high sequence similarity, functionality may not be conserved (c.f. calcitonin between mammals and fish (Clark et al, 2002)). …”

Section: Discussionmentioning

confidence: 99%

Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: Clues from a microarray

Purać

Burns

Thorne

et al. 2008

Journal of Insect Physiology

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The physiology of the Antarctic microarthropod, Cryptopygus antarcticus, has been well studied, particularly with regard to its ability to withstand low winter temperatures. However, the molecular mechanisms underlying this phenomenon are still poorly understood. 1180 sequences (Expressed Sequence Tags or ESTs) were generated and analyzed, from populations of C. antarcticus. This represents the first publicly available sequence data for this species. A sub-set (672 clones) were used to generate a small microarray to examine the differences in gene expression between summer acclimated cold tolerant and non-cold tolerant springtails. Although 60% of the clones showed no sequence similarity to annotated genes in the datasets, of those where putative function could be inferred via database homology, there was a clear pattern of up-regulation of structural proteins being associated with the cold tolerant group. These structural proteins mainly comprised cuticle proteins and provide support for the recent theory that summer SCP variation within Collembola species could be a consequence of moulting, with moulting populations having lowered SCPs.

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Section: Discussionmentioning

confidence: 99%

Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: Clues from a microarray

Purać

Burns

Thorne

et al. 2008

Journal of Insect Physiology

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“…The 5Ј part of both transcripts, comprising exon I and exon II according to ENSEMBL prediction, is identical in calca and cgrp, whereas an alternative third exon is recruited (Fig. 5) as in Fugu (Clark et al, 2002). The probes generated from the different splice variants show an identical expression pattern (data not shown).…”

Section: Calca Expression Visualizes the Ultimobranchial Bodies In Zementioning

confidence: 94%

“…is translated into the preprotein for the calcitonin gene-related peptide (Cgrp; Clark et al, 2002). We amplified both splice variants, calca and cgrp, using different primers (Fig.…”

Section: Calca Expression Visualizes the Ultimobranchial Bodies In Zementioning

confidence: 99%

Analysis of origin and growth of the thyroid gland in zebrafish

Alt

Reibe

Feitosa

et al. 2006

Developmental Dynamics

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141

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The zebrafish thyroid gland shows a unique pattern of growth as a differentiated endocrine gland. Here, we analyze the onset of differentiation, the contribution of lineages, and the mode of growth of this gland. The expression of genes involved in hormone production and the establishment of epithelial polarity show that differentiation into a first thyroid follicle takes place early during embryonic development. Thyroid follicular tissue then grows along the pharyngeal midline, initially independently of thyroid stimulating hormone. Lineage analysis reveals that thyroid follicle cells are exclusively recruited from the pharyngeal endoderm. The ultimobranchial bodies that merge with the thyroid in mammals form separate glands in zebrafish as visualized by calcitonin precursor gene expression. Mosaic analysis suggests that the first thyroid follicle differentiating at 55 hours postfertilization corresponds later to the most anterior follicle and that new follicles are added caudally.

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“…CT has also been suggested to control dietary calcium uptake (Suzuki et al 1999) and inhibit calcium transport in fish gills (Wagner et al 1997, Mukherjee et al 2004. As yet no definite functions of CT (Hirsch et al 2001, Clark et al 2002, Miller 2006, Nag et al 2007) have been established and more work is necessary to establish its functions in fishes.…”

Section: Introductionmentioning

confidence: 99%

Ultimobranchial gland of freshwater catfish, Heteropneustes fossilis, in response to calcitonin administration

et al. 2009

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The absence o!!f a hypocalcemic effect of calcitonin (CT) in fishes has been suggested due to exceedingly high plasma levels of CT; the fish may be saturated with respect of circulating CT and therefore unable to respond to exogenously administered CT. Earlier it has been suggested that a hypocalcemic action of injected CT may be obscured by changes in the release of endogenous CT and other calcium regulating hormones. In this study we have used artificial freshwater, calcium-deficient freshwater and calcium-rich freshwater and injected the fish with CT. The aim behind selecting these media were (i) in calcium-deficient medium there would be reduced circulating levels of CT, (ii) in calciumrich medium there would be diminished secretion of prolactin (this hormone is hypercalcemic in fish), and (iii) by keeping the fish in calcium-rich medium we can test the antihypercalcemic action of CT. Moreover, the present study would reveal the changes in the ultimobranchial gland (UBG) after keeping the fish in all the above three media and/or injecting the fish with CT. Freshwater catfish, Heteropneustes fossilis, were administered intraperitoneally daily with vehicle or 0.5 U/100g body wt of salmon calcitonin (CT) and kept in artificial freshwater, calcium-rich freshwater and calcium-deficient freshwater for 10 days. Blood samples were collected on 1, 3, 5, and 10 days following the treatment and analyzed for serum calcium levels. The ultimobranchial gland (UBG) was also fixed for histological studies on these intervals. In artificial freshwater there was no change in the serum calcium levels of calcitonin-injected fish. The ultimobranchial gland of calcitonin-injected fish exhibited a progressive decrease in the nuclear volume from day 5 onwards. On day 10 vacuolization in the gland was also noticed. In vehicle-injected fish (control) kept in calcium-rich freshwater hypercalcemia has been noticed which persists till the end of the experiment. In calcitonin-treated fish maintained in calcium-rich freshwater there is no change in serum calcium level as compared to vehicle-injected fish. In vehicleinjected fish the UBG depicts decreased staining response and increased nuclear volume at day 5. On day 10 the nuclear volume is further increased and few degenerating cells have been noticed. Calcitonin fails to induce any histological change in the UBG as compared to control. In vehicle-injected fish kept in calcium-deficient freshwater the serum calcium levels decrease from day 1 to day 3. The levels exhibit hypercalcemia on day 10. CT treatment to the fish kept in calcium-deficient freshwater evokes a decrease in the calcium levels on day 1 and day 3. A significant hypercalcemia has been noticed on day 5 and day 10. In vehicle-injected fish kept in calcium-deficient freshwater the UBG reveals a decreased staining response on day 10. In CT-injected fish maintained in calcium-deficient freshwater the UBG depicts an increased nuclear volume and few exhausted cells on day 10. It can be concluded that CT can provoke hypocalcemia o...

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Calcitonin: characterisation and expression in a teleost fish, Fugu rubripes

Cited by 31 publications

References 53 publications

Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: Clues from a microarray

Cold hardening processes in the Antarctic springtail, Cryptopygus antarcticus: Clues from a microarray

Analysis of origin and growth of the thyroid gland in zebrafish

Ultimobranchial gland of freshwater catfish, Heteropneustes fossilis, in response to calcitonin administration

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