Calcitonin directly increases adrenocorticotropic hormone-stimulated corticosterone production in the hen adrenal gland

Abstract

The present study was performed to elucidate whether the receptor for calcitonin (CT) exists in the adrenocortical cells of hens and to determine the effect of CT on adrenocorticotropic hormone (ACTH)-stimulated corticosterone production in its cell. The binding site of CT in the membrane fraction of the adrenal gland in hens was determined using a [125I]CT binding assay system. The binding properties in the adrenal gland satisfied the criteria of a receptor-ligand interaction in terms of specificity, reversibility, and saturation. When the cortical cells were incubated in vitro with chicken ACTH in the presence of CT, greater corticosterone production was observed. The result suggested that CT acts directly on the adrenocortical cells via its receptor binding and increases responsiveness of ACTH on corticosterone production in the laying hen.

Calcitonin receptor binding in the hen anterior pituitary during an oviposition cycle

“…It has been reported that CT enhances the maximal secretion of ACTH stimulated by corticotropinreleasing hormone in the hen anterior pituitary (Nakayama et al 2010a), and increases corticosterone production stimulated by ACTH in the hen adrenal gland (Nakagawa-Mizuyachi et al 2009). The concentration of corticosterone in blood increases at the time of oviposition (Rice et al 1985).…”

Effect of calcitonin on adrenocorticotropic hormone secretion stimulated by corticotropin‐releasing hormone in the hen anterior pituitary

“…Anterior pituitary cells were incubated in vitro using the same method as reported earlier on the incubation of adrenocortical cells of the hen (Nakagawa‐Mizuyachi et al . 2009).…”

“…1999). Recently, it has been shown that the receptor for CT exists in the chicken adrenal gland and CT directly increases corticosteone production stimulated by adrenocorticotropic hormone (ACTH) through its receptor (Nakagawa‐Mizuyachi et al . 2009).…”

Parathyroid hormone-related peptide directly increases adrenocorticotropic hormone secretion from the anterior pituitary in hens

“…Receptors for calcitonin (Nakagawa-Mizuyachi et al, 2009) and PTHrP (Kawashima et al, 2005) exist in the chicken adrenal gland, and these hormones directly increase corticosterone production stimulated by ACTH through their receptors. Recently, it was reported that calcitonin enhances the secretion of ACTH from the anterior pituitary via its receptor (Nakayama et al, 2010b).…”