Calcitonin gene-related peptides relax guinea pig and rat gastric smooth muscle

Katsoulis, Stavros; Conlon, J. Michael

doi:10.1016/0014-2999(89)90612-2

Cited by 52 publications

(25 citation statements)

References 23 publications

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“…The stomach was taken out and the fundus was separated from the whole organ. Longitudinal muscle strips were prepared from the fundic region as previously described (Katsoulis & Conlon, 1989 Characterization of the contracting effect ofgalanin on ratfundus strips…”

Section: Animals and Tissue Preparationsmentioning

confidence: 99%

Effects of galanin, its analogues and fragments on rat isolated fundus strips

Katsoulis

Schmidt

Schwörer

et al. 1990

British J Pharmacology

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1 Rat and porcine galanin (rGal and pGal) produced dose-dependent contraction of rat fundus strips in a concentration range of 6 nM-100 nm. 2 The stimulatory effect of rGal on rat fundus strips was not modified in the presence of somatostatin (250nM), naloxone (1 uM), guanethidine (10 CM), a mixture of propranolol (3 #M) and phentolamine (3puM), tetrodotoxin (1 pM), indomethacin (10pM), atropine (1 M), a mixture of methysergide (2.5,UM) and ketanserin (2.5 uM), a mixture of mepyramine (10,UM) and cimetidine (10M), and saralasin (10puM) The order of potency of the galanin peptides, analogues and fragments to contract rat fundus strips was:The data originating from our structure-activity study suggest that the C-terminal portion of Gal contributes mainly to the afflinity of Gal receptors whereas the N-terminal portion of Gal is responsible for the full activation of Gal receptors in this tissue. In particular the amino acids in position 1 and 2 of Gal (Gly-Trp) appear to be essential for binding and intrinsic activity.

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Section: Animals and Tissue Preparationsmentioning

confidence: 99%

Effects of galanin, its analogues and fragments on rat isolated fundus strips

Katsoulis

Schmidt

Schwörer

et al. 1990

British J Pharmacology

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“…It is the most powerful vasoactive substance known and it increases mucosal blood flow. 4,5) CGRP is known to coexist with tachykinins in the population of sensory neurons in humans. 6) Substance P is widely distributed in the central and peripheral nervous system and in the enteroendocrine cells of the gut 7) and it participates in the regulation of gastrointestinal motility, secretion, and blood flow.…”

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confidence: 99%

Dai-kenchu-to Raises Levels of Calcitonin Gene-Related Peptide and Substance P in Human Plasma

Sato

Katagiri

Inoue

et al. 2004

Biological & Pharmaceutical Bulletin

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Sensory afferent neurons in the gastrointestinal mucosa regulate neuropeptides [calcitonin gene-related peptide (CGRP), substance P, etc.], which play various physiologic roles and are gastroprotective. To determine whether the pharmacologic effects of Dai-kenchu-to (DKCT) on the gastrointestine are due to changes in gastrointestinal mucosa regulatory peptide levels, we examined the effects of the DKCT on the levels of CGRP-like immunoreactive substances (IS) and substance P-IS in plasma taken from five healthy subjects. A single oral administration of DKCT 7.5 g caused significant increases in plasma CGRP-IS at 40 min, and in substance P-IS levels at 20 and 60 min, compared with a placebo group. The present study may indicate that the pharmacologic action of DKCT is closely related to changes in CGRP-and substance P-IS levels.

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“…CGRP has several potent biological activities, including vasodilation, and in the gastric mucosa its vasodilatory effects following stimulated release from the extrinsic sensory innervation is considered to serve as an important protective mechanism for maintaining gastric mucosal integrity. 15,16) CGRP is a potent gastrointestinal vasodilator and causes increases in the gastrointestinal mucosal blood flow. 17) Substance P coexists with CGRP in the sensory afferent neurons in the gastrointestinal mucosa.…”

Section: Resultsmentioning

confidence: 99%

Effect of a Single Treatment with Keishininjinto on Plasma Levels of Gut-regulatory Peptides in Healthy Subjects

Sato

Katagiri

Inoue

et al. 2007

JOURNAL OF HEALTH SCIENCE

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A traditional Japanese Kampo medicine Keishininjinto has been empirically used for the treatment of headache, chronic gastroenteritis, gastric atony, and waterly diarrhea often accompanying abdominal pain, cold, fever, and headache. One of the mechanisms of the empirical effects is assumed to be due to local changes in gut-regulated peptide levels. We studied the effects of Keishininjinto on calcitonin gene-related peptide (CGRP)-, substance P-, vasoactive intestinal polypeptides (VIP)-, motilin-, and somatostatin-like immunoreactive substances (IS) in plasma taken from healthy subjects. Keishininjinto (6.0 g) or placebo was orally administered to five healthy males. Blood samples were taken before, and at 20, 40, 60, 90, 120, 180, and 240 min after administration, followed by the extracting procedure, and submitted to a highly sensitive enzyme immunoassay system for CGRP-, substance P-, VIP-, motilin-, and somatostatin-IS. A single oral administration of Keishininjinto caused significant increases in plasma CGRP-, and substance P-IS levels compared with placebo. On the other hand, a single oral administration of Keishininjinto transiently caused decreases in plasma VIP-IS levels compared with placebo. However, Keishininjinto had no effects on plasma motilin-, and somatostatin-IS levels. In this study, we hypothesized that Keishininjinto might stimulate capsaicinsensitive afferent nerves and improve gastrointestinal mucosal blood flow, and that might affect intestinal secretion and motility in neuronal reflexes.

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Calcitonin gene-related peptides relax guinea pig and rat gastric smooth muscle

Cited by 52 publications

References 23 publications

Effects of galanin, its analogues and fragments on rat isolated fundus strips

Effects of galanin, its analogues and fragments on rat isolated fundus strips

Dai-kenchu-to Raises Levels of Calcitonin Gene-Related Peptide and Substance P in Human Plasma

Effect of a Single Treatment with Keishininjinto on Plasma Levels of Gut-regulatory Peptides in Healthy Subjects

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