Calcitonin Prevents CCl4-Induced Hydroperoxide Generation and Cytotoxicity Possibly through C1b Receptor in Rat Hepatocytes

Chen, Shaoyan; Morimoto, Shigeto; Tamatani, Michio; Fukuo, Keisuke; Nakahashi, Takeshi; Nishibe, Akira; Jiang, Bingbing; Ogihara, Toshio

doi:10.1006/bbrc.1996.0154

Cited by 9 publications

(4 citation statements)

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“…Many studies implicate LPO in the cellular damage of HepG2 cells produced by a variety of toxic agents [Lima et al, ]. The elevation of ALT and AST activities observed in the current study could be due to the direct effect of CCl 4 or by the generation of trichloromethyl (*CCl 3 ) radicals by cytochrome P‐450, and subsequent LPO effects that are dependent on its concentration, the duration of exposure and the sensitivity of the in vitro system to which it is added [Chen et al, ]. Consistent with the present results, the excess production of free radicals increased the utilization of antioxidants to prevent LPO and deplete antioxidants in cell lysate and it has been established that cellular oxidative stress can be often preceded by depletion of endogenous antioxidants [Rahal et al, ].…”

Section: Discussionsupporting

confidence: 89%

Phytochemical Investigation, Antitumor Activity, and Hepatoprotective Effects of Acrocarpus fraxinifolius Leaf Extract

El-Nashar

Eldahshan

Elshawi

et al. 2017

Drug Development Research

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Preclinical Research Nine known phenolic compounds were isolated from an aqueous methanolic extract of Acrocarpus fraxinifolius Weight and Arn leaves (AFL) family Fabaceae. This extract of AFL contained approximately 169 mg gallic acid/g as assessed by HPLC. The AFL extract had marginal antitumor activity (IC > 200 µL/mL) but showed a concentration-dependent hepatoprotective effect against CCl -induced hepatotoxicity in vitro. Cell viability was increased, ALT and AST activity declined and reduced GSH concentration and SOD activity were restored as compared with silymarin. In vivo concurrent administration of AFL extract (500 mg/kg po) showed a hepatoprotective effect against gamma irradiation and CCl as evidenced by reduction of TNF-α, interleukin-6, malondialdehyde, nitric oxide, DNA fragmentation, caspase-3 activity, and downregulation of its m-RNA level and decreased proapoptotic protein Bax expression. AFL extract enhanced glutathione peroxidase, superoxide dismutase, and catalase activities, reduced glutathione concentrations and upregulated the expression of antiapoptotic Bcl-2. The extract could ameliorate hepatic injuries induced by gamma irradiation and CCl in rats suggesting potent hepatoprotective activity. Drug Dev Res 78 : 210-226, 2017. © 2017 Wiley Periodicals, Inc.

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Section: Discussionsupporting

confidence: 89%

Phytochemical Investigation, Antitumor Activity, and Hepatoprotective Effects of Acrocarpus fraxinifolius Leaf Extract

El-Nashar

Eldahshan

Elshawi

et al. 2017

Drug Development Research

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“…The in vitro liver damage caused by CCl 4 has been hypothesized to be caused by two different mechanisms, depending on the concentration used and the exposure time: a direct solvent effect of the molecule itself or an indirect effect through the generation of free radicals and subsequent lipid peroxidation [27]. …”

Section: Discussionmentioning

confidence: 99%

In vitro assessment of hepatoprotective agents against damage induced by acetaminophen and CCl4

Torres-González

Minsky

Espinosa

et al. 2017

BMC Complement Altern Med

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BackgroundIn vitro bioassays are important in the evaluation of plants with possible hepatoprotective effects. The aims of this study were to evaluate the pretreatment of HepG2 cells with hepatoprotective agents against the damage induced by carbon tetrachloride (CCl4) and paracetamol (APAP).MethodsAntioxidative activity was measured using an assay to measure 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging. The in vitro hepatotoxicity of CCl4 and APAP, and the cytotoxic and hepatoprotective properties of silymarin (SLM), silybinin (SLB), and silyphos (SLP) were evaluated by measuring cell viability; activities of aspartate aminotransferase (AST), alanine aminotransferase (ALT), and lactate dehydrogenase (LDH); total antioxidant capacity (TAOxC); and reduced glutathione (GSH), superoxide dismutase (SOD), and lipid peroxidation (malondialdehyde (MDA) levels).ResultsOnly SLB and SLM showed strong antioxidative activity in the DPPH assay (39.71 ± 0.85 μg/mL and 14.14 ± 0.65 μg/mL, respectively). CCl4 induced time- and concentration-dependent changes. CCl4 had significant effects on cell viability, enzyme activities, lipid peroxidation, TAOxC, and SOD and GSH levels. These differences remained significant up to an exposure time of 3 h. APAP induced a variety of dose- and time-dependent responses up to 72 h of exposure. SLM, SLB, and SLP were not cytotoxic. Only SLB at a concentration of 100 μg/mL or 150 μg/mL significantly decreased the enzyme activities and MDA level, and prevented depletion of total antioxidants compared with CCl4.ConclusionsCCl4 was more consistent than APAP in inducing cell injury. Only SLB provided hepatoprotection. AST, LDH, and MDA levels were good markers of liver damage.

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“…The antihepatotoxic effects of the methanolic extract and isolated compounds were assayed on CCl 4 -treated cells. The CCl 4 concentration used for cell culture treatment was previously determined and chosen because of its ability of induce up to 75% cell culture mortality (Chen et al 1996;Rodeiro et al 2008;Donfack et al 2010). The methanolic extract and isolated compounds from F. gnaphalocarpa were not completely soluble in aqueous medium and had to be emulsified in dimethyl sulfoxide (DMSO) prior to their addition to the cell culture medium.…”

Section: Chemicalsmentioning

confidence: 99%

In vitro hepatoprotective and antioxidant activities of crude extract and isolated compounds from Ficus gnaphalocarpa

et al. 2010

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The in vitro hepatoprotective effect of the methanolic extract from Ficus gnaphalocarpa (Miq.) Steud. ex A. Rich (Moraceae) on the CCl₄-induced liver cell damage as well as the possible antioxidant mechanisms involved in this protective effect, were investigated. The phytochemical investigation of this methanolic extract led to the isolation of six compounds identified as: betulinic acid (1); 3-methoxyquercetin (2); catechin (3); epicatechin (4); quercetin (5); and quercitrin (6). The hepatoprotective activity of these compounds was tested in vitro against CCl₄-induced damage in rat hepatoma cells. In addition, radical-scavenging activity, β-carotene-linoleic acid model system, ferric-reducing antioxidant parameter and microsomal lipid peroxidation assays were used to measure antioxidant activity of crude extract and isolated compounds. Silymarin and trolox were used as standard references and, respectively, exhibited significant hepatoprotective and antioxidant activities. (5), (6) and (2) showed significant antioxidant and hepatoprotective activities as indicated by their ability to prevent liver cell death and lactate dehydrogenase leakage during CCl₄ intoxication. These results suggest that the protective effects of crude extract of F. gnaphalocarpa against the CCl₄-induced hepatotoxicity possibly involve the antioxidant effect of these compounds.

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Calcitonin Prevents CCl4-Induced Hydroperoxide Generation and Cytotoxicity Possibly through C1b Receptor in Rat Hepatocytes

Cited by 9 publications

References 0 publications

Phytochemical Investigation, Antitumor Activity, and Hepatoprotective Effects of Acrocarpus fraxinifolius Leaf Extract

Phytochemical Investigation, Antitumor Activity, and Hepatoprotective Effects of Acrocarpus fraxinifolius Leaf Extract

In vitro assessment of hepatoprotective agents against damage induced by acetaminophen and CCl4

In vitro hepatoprotective and antioxidant activities of crude extract and isolated compounds from Ficus gnaphalocarpa

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