Calcitriol inhibits tumor necrosis factor alpha and macrophage inflammatory protein-2 during lipopolysaccharide-induced acute lung injury in mice

Tan, Zhu-Xia; Chen, Yuan-Hua; Xu, Shen; Qin, Hou-Ying; Wang, Hua; Zhang, Cheng; D, Xu; Zhao, Hui

doi:10.1016/j.steroids.2016.05.005

Cited by 21 publications

(20 citation statements)

References 52 publications

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“…Resveratrol (Bigagli et al, 2017), chloral hydrate (Cai et al, 2016) and paracetamol (Vuong et al, 2019) have antiinflammatory and anti-oxidant effects in LPS-stimulated RAW 264.7 macrophage cells in vitro. Calcitriol, the active form of vitamin D, has been confirmed that it can reduce the infiltration of inflammatory cells and attenuate the elevation of TNF-α during LPS-induced acute lung injury in mice (Tan et al, 2016). Similarly, in the lung injury model of mice (Metsola et al, 2014), adenosine regulates endothelial permeability and plays a role of anti-inflammation via its receptors.…”

Section: Research Articlementioning

confidence: 95%

Washed microbiota transplantation vs. manual fecal microbiota transplantation: clinical findings, animal studies and in vitro screening

Zhang

Zhao³

et al. 2020

Protein Cell

182

178

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Fecal microbiota transplantation (FMT) by manual preparation has been applied to treat diseases for thousands of years. However, this method still endures safety risks and challenges the psychological endurance and acceptance of doctors, patients and donors. Population evidence showed the washed microbiota preparation with microfiltration based on an automatic purification system followed by repeated centrifugation plus suspension for three times significantly reduced FMT-related adverse events. This washing preparation makes delivering a precise dose of the enriched microbiota feasible, instead of using the weight of stool. Intraperitoneal injection in mice with the fecal microbiota supernatant obtained after repeated centrifugation plus suspension for three times induced less toxic reaction than that by the first centrifugation following the microfiltration. The toxic reactions that include death, the change in the level of peripheral white blood cells, and the proliferation of germinal center in secondary lymphoid follicles in spleen were noted. The metagenomic next-generation sequencing (NGS) indicated the increasing types and amount of viruses could be washed out during the washing process. Metabolomics analysis indicated metabolites with pro-inflammatory effects in the fecal microbiota supernatant such as leukotriene B4, corticosterone, and prostaglandin G2 could be removed by repeated washing. Near-infrared absorption spectroscopy could be served as a rapid detection method to control the quality of the washingprocess. In conclusion, this study for the first time provides evidence linking clinical findings and animal experiments to support that washed microbiota transplantation (WMT) is safer, more precise and more quality-controllable than the crude FMT by manual.

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Section: Research Articlementioning

confidence: 95%

Washed microbiota transplantation vs. manual fecal microbiota transplantation: clinical findings, animal studies and in vitro screening

Zhang

Zhao³

et al. 2020

Protein Cell

182

178

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“…73 Its ability to counteract microbial infections and inflammation depends on the VDR polymorphism and on vitamin D availability, [74][75][76] in addition to the CYP450 hydroxylase expression, 77 where vitamin D is able to induce an anti-inflammatory response. 47,78,79 At the molecular level, a recent pilot study reported that vitamin D deficiency affected the toll-like receptor (TLR) expression on peripheral blood mononuclear cells (PBMCs).…”

Section: Role In Innate Immunitymentioning

confidence: 99%

The Role of Vitamin D in the Immune System as a Pro-survival Molecule

Chirumbolo

Bjørklund

Sboarina

et al. 2017

Clinical Therapeutics

106

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“…in the lung tissue, endotoxin attracts and activates macrophages to produce and release large amounts of inflammatory cytokines. The accumulation of a large number of macrophages in the lungs leads to excessive inflammation and thus aggravates the tissue damage [8,9]. Our results showed that the macrophages were significantly accumulated in the pulmonary alveolar and the interval space in the rat models of liver fibrosis, with elevated levels of TNF-α in the homogenate.…”

Section: Discussionmentioning

confidence: 75%

Protective effects of emodin on lung injuries in rat models of liver fibrosis

Zhang

Wang

et al. 2019

Open Life Sciences

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ObjectiveThe aim of this study is to investigate the protective effects of emodin (EMD) on the lung injuries in the rat models of liver fibrosis.MethodsLiver fibrosis was established in rats and the effect of intervention using EMD treatment was determined. Liver and lung weight coefficients were measured and lung content of TNF-α (tumor necrosis factor α), MDA (malondialdehyde), NO (nitric oxide), and ONOO- (peroxynitrite) were determined. Finally, histopathological changes were evaluated.ResultsCompared with the normal control group, the lung weight coefficient was significantly increased in the fibrosis model group. Moreover, pulmonary edema and inflammatory responses were observed. Levels of TNF-α, MDA, NO, and ONOO- in the lung homogenate were significantly increased in the fibrosis model group. After EMD treatment, the lung weight coefficients were significantly reduced. Moreover, pathological changes in the lung tissue were dramatically alleviated. Levels of TNF-α, MDA, NO, and ONOO- were significantly decreased.ConclusionEMD exhibits protective effects against lung injuries in a rat model of liver fibrosis.

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Calcitriol inhibits tumor necrosis factor alpha and macrophage inflammatory protein-2 during lipopolysaccharide-induced acute lung injury in mice

Cited by 21 publications

References 52 publications

Washed microbiota transplantation vs. manual fecal microbiota transplantation: clinical findings, animal studies and in vitro screening

Washed microbiota transplantation vs. manual fecal microbiota transplantation: clinical findings, animal studies and in vitro screening

The Role of Vitamin D in the Immune System as a Pro-survival Molecule

Protective effects of emodin on lung injuries in rat models of liver fibrosis

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