1982
DOI: 10.1085/jgp.79.4.603
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Calcium accumulation by the sarcoplasmic reticulum in two populations of chemically skinned human muscle fibers. Effects of calcium and cyclic AMP.

Abstract: In previous efforts to characterize sarcoplasmic reticulum function in human muscles, it has not been possible to distinguish the relative contributions of fast-twitch and slow-twitch fibers . In this study, we have used light scattering and 45Ca to monitor Ca accumulation by the sarcoplasmic reticulum of isolated, chemically skinned human muscle fibers in the presence and absence of oxalate . Oxalate (5 mM) increased the capacity for Ca accumulation by a factor of 35 and made it possible to assess both rate o… Show more

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Cited by 72 publications
(67 citation statements)
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“…Muscle fibers were chemically skinned as previously described (7,28,38). Briefly, muscles were tied to a wooden stick, stretched to 110 -120% of slack length, and quickly immersed into an ice-cold skinning solution containing 170 mM K-propionate, 2.5 mM Mg-propionate, 2.5 mM Na2K2ATP, 5 mM K2EGTA, 10 mM imidazole buffer, pH 7.0.…”
Section: Methodsmentioning
confidence: 99%
“…Muscle fibers were chemically skinned as previously described (7,28,38). Briefly, muscles were tied to a wooden stick, stretched to 110 -120% of slack length, and quickly immersed into an ice-cold skinning solution containing 170 mM K-propionate, 2.5 mM Mg-propionate, 2.5 mM Na2K2ATP, 5 mM K2EGTA, 10 mM imidazole buffer, pH 7.0.…”
Section: Methodsmentioning
confidence: 99%
“…Calcium uptake by the SR has been analyzed using membrane fractions enriched in SR vesicles obtained by differential centrifugation of muscle homogenates. Calcium uptake is faster in fast than in slow fibers (522,688,756), and this is determined partly by the greater SR volume or SR surface area and partly by the presence of specific transport proteins.…”
Section: Calcium Uptake By Srmentioning
confidence: 99%
“…Normal and regenerating fibres were chemically skinned as previously described (Salviati et al 1982;Midrio et al 1997;Danieli-Betto et al 2000). Briefly, muscles were tied to a wooden stick and quickly immersed into an ice-cold skinning solution containing (mM): potassium propionate 170, magnesium propionate 2.5, Na 2 ATP 2.5, K 2 EGTA 5 and imidazole buffer 10; pH 7.0.…”
Section: Chemical Skinning Of Muscle Fibresmentioning
confidence: 99%
“…2+ uptake measurements Ca 2+ uptake by the sarcoplasmic reticulum was measured at room temperature (22-24°C) using a light-scattering method (Sorenson et al 1980;Salviati et al 1982;Midrio et al 1997). Fibres were mounted in a chamber in a relaxing solution containing (mM): potassium propionate 170, magnesium propionate 2.5, Na 2 ATP 5, K 2 EGTA 5 and imidazole buffer 10; pH 7.0, and stretched to 180 % of slack length to avoid interference in lightscattering measurements caused by actin-myosin interactions.…”
Section: Camentioning
confidence: 99%