Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor

Hunt, Nicola; Shelton, Richard M.; Henderson, Deborah J.; Grover, Liam M.

doi:10.1089/ten.tea.2012.0197

Cited by 40 publications

(15 citation statements)

References 57 publications

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“…Initially, this was thought to be principally a consequence of the modulus of the materials, but more recently reports have suggested that this can be modified by changing the distribution of grafted adhesion moieties and the nature of the interactions that may be formed with the polymer chain . It has become clear that the level of entrapment that can be provided by the relatively stiff quiescently gelled matrix can have a significant influence on biological properties, including the capacity of cells to proliferate, maintain specific phenotypes or even secrete functional protein molecules as shown in Figure …”

Section: The Delivery Of Biological Therapeutics Using Hydrogelsmentioning

confidence: 99%

Structuring of Hydrogels across Multiple Length Scales for Biomedical Applications

et al. 2018

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The development of new materials for clinical use is limited by an onerous regulatory framework, which means that taking a completely new material into the clinic can make translation economically unfeasible. One way to get around this issue is to structure materials that are already approved by the regulator, such that they exhibit very distinct physical properties and can be used in a broader range of clinical applications. Here, the focus is on the structuring of soft materials at multiple length scales by modifying processing conditions. By applying shear to newly forming materials, it is possible to trigger molecular reorganization of polymer chains, such that they aggregate to form particles and ribbon-like structures. These structures then weakly interact at zero shear forming a solid-like material. The resulting self-healing network is of particular use for a range of different biomedical applications. How these materials are used to allow the delivery of therapeutic entities (cells and proteins) and as a support for additive layer manufacturing of larger-scale tissue constructs is discussed. This technology enables the development of a range of novel materials and structures for tissue augmentation and regeneration.

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Section: The Delivery Of Biological Therapeutics Using Hydrogelsmentioning

confidence: 99%

Structuring of Hydrogels across Multiple Length Scales for Biomedical Applications

et al. 2018

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“…After tissue injury, fibroblasts migrate towards the wound, enhance cell division near the edge of the wound and secrete extracellular matrix (ECM) to support further cell migration [29]. Fibroblasts can express vascular endothelial growth factor (VEGF), which plays a major role in angiogenesis and acts as a chemo-attractant [30]. Therefore, fibroblast viability and proliferation is essential in evaluating the biocompatibility of PETOx-co-PEI.…”

Section: Introductionmentioning

confidence: 99%

In vitro study of partially hydrolyzed poly(2-ethyl-2-oxazolines) as materials for biomedical applications

Shah

Kroneková

Zahoranová

et al. 2015

J Mater Sci: Mater Med

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Polymers based on 2-oxazoline, such as poly(2-ethyl-2-oxazolines) (PETOx), are considered to be a type of 'pseudopeptide' with the ability to form novel biomaterials. The hydrolysis of PETOx was carried out to evaluate its use in biomedical applications. In the present work, PETOx samples with a range of molar masses were prepared by living cationic polymerization. Hydrolysis was carried out at time intervals ranging from 15 to 180 min to prepare copolymers with different amounts of ethylene imine units. (1)H NMR spectroscopy was used to identify the structure of the hydrolyzed polymers. The dependence of in vitro cell viability on the degree of hydrolysis was determined using three different model cell lines, namely, mouse embryonic 3T3 fibroblasts, pancreatic βTC3 cells, and mouse lymphoid macrophages P388.D1. It was demonstrated that increasing the degree of hydrolysis decreased cell viability for all cell types. Fibroblast cells displayed the highest tolerance; additionally, the effect of polymer size showed no observable significance. Macrophage cells, immune system representatives, displayed the highest sensitivity to contact with hydrolyzed PETOx. The effect of polymer hydrolysis, polymer concentration and the incubation time on cell viability was experimentally observed. Confocal laser-scanning microscopy provided evidence of cellular uptake of pyrene-labeled (co)polymers.

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“…Other polyelectrolytes tested include chitosan (IG with tripolyphosphate), carboxymethylcellulose (IG with aluminum), pectin (IG with calcium), gelan gum (IG with calcium), and alginate and cellulose phosphate (IG with calcium), polyphosphazene (IG with calcium). For example, calcium alginate hydrogels have been used for delivery of vascular endothelial growth factor (VEGF), achieving an encapsulation efficiency of 30-67% and constant release for up to 14 days [43].…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Stabilization Challenges and Formulation Strategies Associated with Oral Biologic Drug Delivery Systems

Truong‐Le¹,

Lovalenti²,

Abdul‐Fattah³

2015

Advanced Drug Delivery Reviews

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Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor

Cited by 40 publications

References 57 publications

Structuring of Hydrogels across Multiple Length Scales for Biomedical Applications

Structuring of Hydrogels across Multiple Length Scales for Biomedical Applications

In vitro study of partially hydrolyzed poly(2-ethyl-2-oxazolines) as materials for biomedical applications

Stabilization Challenges and Formulation Strategies Associated with Oral Biologic Drug Delivery Systems

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