Calcium Alleviates Temperature Stress by Regulating Nitrogen and Respiratory Metabolism in Malus baccata Roots

Hong, Su Young; Li, Lijie; Ma, Huaiyu; Lyu, Deguo; Sun, Jing

doi:10.17957/ijab/15.0084

Cited by 10 publications

(7 citation statements)

References 23 publications

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“…6 Calcicole plants require more calcium, whereas calcifuge plants require less calcium. 7,8 The growth of calcifuge plants, such as sphagnum, rhododendron and blueberry, needs a lower calcium and higher H + concentration [9][10][11] . In general, tea plants are also considered to be calcifuge plants, which are sensitive to calcium and pH, preferring relatively low calcium levels and growing well in acid soils.…”

Section: Introductionmentioning

confidence: 99%

Metabolomics analysis reveals the responses of tea plants to excessive calcium

et al. 2021

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BACKGROUND: The proper growth and development of tea plants requires moderately acidic soils and relatively low calcium levels, and excessive calcium at high pH can damage tea plant roots. To reveal the effects of calcium on the responses of tea plant to three pH levels (3.5, 5.0 and 6.5), a repeated test of two factors was designed. RESULTS: Root growth and elemental analysis indicated that excessive calcium improved the growth of tea roots at low pH conditions, whereas it did not harm the growth of tea roots under normal and high pH conditions, especially at pH 6.5. Excessive calcium antagonized the absorption and utilization of magnesium by tea plants. Gas chromatography-mass spectrometry results showed that the addition of Ca 2+ resulted in the primary metabolism in roots being more active at a low pH level. By contrast, it had obvious adverse effects on the accumulation of root metabolites with high calcium treatment at normal or high pH. Differential metabolites identified using ultra-performance liquid chromatography quadrupole time of flight mass spectrometry indicated that flavonoids demonstrated the largest number of changes, and their biosynthesis was partially enriched with excessive calcium at low and high pH conditions, whereas it was down-regulated under normal pH conditions. Kaempferol 3-(2 0 -rhamnosyl-6 0 -acetylgalactoside) 7-rhamnoside, quercetin 3-(6 0 -sinapoylsophorotrioside) and delphinidin 3-(3 0 -p-coumaroylglucoside) showed the greatest increase. The results of gene expression related to root growth and calcium regulation were consistent with root growth and root metabolism. CONCLUSION:The overall results demonstrated that high Ca concentrations further aggravate the detrimental effects of high pH to tea roots. However, it is interesting that excessive calcium reduced the harm of a low pH on tea root growth to some extent.

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Section: Introductionmentioning

confidence: 99%

Metabolomics analysis reveals the responses of tea plants to excessive calcium

et al. 2021

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“…In a similar observation in this study, the addition of calcium to roots could alleviate the inhibition of photosynthesis induced by changing temperature. These results indicate that, under temperature stress, photoinhibition in leaves can be alleviated by enhancing the function of roots by adding calcium [ 1 , 2 ]. We also performed differential proteomic analysis of M. baccata leaves under conditions of rapid changes in temperature along with calcium treatment, the results of which are discussed below.…”

Section: Discussionmentioning

confidence: 99%

“…In the cool apple-producing areas of northern China, the air temperature during early spring fluctuates in a rapid and drastic manner, which is harmful to the formation of buds and leaves, and severely impedes the growth and development of apple trees [ 1 ]. Previous studies have investigated some physiological-biochemical characteristics of Malus baccata roots under temperature stress, and have shown that changing temperature decreases root vitality and has a negative effect on mitochondrial function and nitrogen and respiratory metabolism [ 1 , 2 ]. The changing temperature stress can also damage the photosynthetic function and affect the anti-oxidation activities of leaves in M. baccata [ 3 ].…”

Section: Introductionmentioning

confidence: 99%

Differential Proteomic Analysis Reveals the Effect of Calcium on Malus baccata Borkh. Leaves under Temperature Stress

Hong²,

et al. 2017

IJMS

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In the cool apple-producing areas of northern China, air temperature during early spring changes in a rapid and dramatic manner, which affects the growth and development of apple trees at the early stage of the growing season. Previous studies have shown that the treatment of calcium can increase the cold tolerance of Malus baccata Borkh., a widely-used rootstock apple tree in northern China. To better understand the physiological function of calcium in the response of M. baccata to temperature stress, we analyzed the effect of calcium treatment (2% CaCl2) on M. baccata leaves under temperature stress. Physiological analysis showed that temperature stress aggravated membrane lipid peroxidation, reduced chlorophyll content and induced photo-inhibition in leaves, whereas these indicators of stress injuries were alleviated by the application of calcium. An isobaric tags for relative and absolute quantitation (iTRAQ)-based proteomics approach was used in this study. Among the 2114 proteins that were detected in M. baccata leaves, 41, 25, and 34 proteins were differentially regulated by the increasing, decreasing, and changing temperature treatments, respectively. Calcium treatment induced 9 and 15 proteins after increasing and decreasing temperature, respectively, in comparison with non-treated plants. These calcium-responsive proteins were mainly related to catalytic activity, binding, and structural molecule activity. Hierarchical cluster analysis indicated that the changes in abundance of the proteins under increasing temperature and changing temperature treatments were similar, and the changes in protein abundance under decreasing temperature and increasing temperature with calcium treatment were similar. The findings of this study will allow a better understanding of the mechanisms underlying the role of calcium in M. baccata leaves under temperature stress.

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“…Combined with Ca 2+ alleviated the decrease of total amino acids, essential amino acids, and protein contents limited by the intensity of SAR2(pH 3.0), we can speculate that exogenous Ca 2+ eliminate the disruption of GS/GOGAT balance for maintaining the nutritional quality of rice under SAR. Under other abiotic stresses, the regulatory effects of exogenous Ca 2+ on the activities of NR, GOGAT, and GDH were also reported (Liang et al 2011, Su et al 2016.…”

Section: Of Exogenous Ca 2+ On Activities and Genes Expression Of Key...mentioning

confidence: 94%

Exogenous Ca2+ Regulating Nitrogen Assimilation for Improving Yield and Quality of Rice under Acid Rain Stress

Zhang

Liang

2022

Preprint

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Acid rain threatens crop yield and nutritional quality, and Ca2+ can regulate plant responses to abiotic stresses. To improve the yield and nutritional quality of crops under acid rain stress, we applied exogenous Ca2+ to regulate nitrogen assimilation in rice seedlings under simulated acid rain stress (SAR, pH 4.5 or 3.0), taking yield and nutritional quality of rice as evaluation criteria. We found that Ca2+ (5 mM) maintained the total nitrogen content of rice at the seedling and booting stages to alleviate the inhibitory effect of SAR on rice yield. Meanwhile, Ca2+ improved the activity of glutamate synthase to eliminate the disruption of glutamine synthetase/glutamate synthase balance under SAR. It decreased the efficiency of nitrogen assimilation, thereby reducing the inhibition of essential amino acid content in rice. The mitigation effect on pH 4.5 SAR was better than pH 3.0 SAR. Overall, Ca2+ may reduce the negative effect of acid rain on yield and nutritional quality of crops.

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Calcium Alleviates Temperature Stress by Regulating Nitrogen and Respiratory Metabolism in Malus baccata Roots

Cited by 10 publications

References 23 publications

Metabolomics analysis reveals the responses of tea plants to excessive calcium

Metabolomics analysis reveals the responses of tea plants to excessive calcium

Differential Proteomic Analysis Reveals the Effect of Calcium on Malus baccata Borkh. Leaves under Temperature Stress

Exogenous Ca2+ Regulating Nitrogen Assimilation for Improving Yield and Quality of Rice under Acid Rain Stress

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