Calcium and photosynthetic oxygen evolution in cyanobacteria

Piccioni, Richard G.; Mauzerall, D.

doi:10.1016/0005-2728(78)90061-0

Cited by 58 publications

(14 citation statements)

References 23 publications

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“…For example, maximum activity required the continued presence of an appropriate osmoticum following cell disruption; maximum activity required exposure of the isolated membranes to Ca2+; the Ca2+-stimulated activity was not further increased by a 'supernatant' factor when photosynthetic membranes were separated from other cytoplasmic components, in contrast to previous observations with Cyanobacteria [9]. However, our results differ from those reported [3,10] in several important ways. For example, in our preparations maximum activity required Ca" during cell breakage, and immediately prior to assays, but not during the intermediate period when membrane preparations were held near 0°C prior to assays; our rates routinely approached or exceeded 200 lrmol O2 .mg chlorophyll-' .h-', -20~times the rate seen previously with French pressure cell preparations of Phormidium and &times the previous rate with Anacystis; our preparations were fully sensitive to inhibition by low concentrations of DCMU.…”

Section: Discussioncontrasting

confidence: 99%

“…A Ca"' requirement for O2 evolution in the Cyanobacterium,Phormidium luridum was characterized [3] and similar Ca" effects in Anacystis niduians and Anabaena flosaquae noted. Our preparations are similar to theirs in several respects.…”

Section: Discussionmentioning

confidence: 90%

“…In most cases Mg2+ appears to be the physiological ion of importance although ATPase activity in certain photosynthetic coupling factor preparations is Cap-dependent [I]. A direct role for Ca2+ in electron transport activities of isolated membranes from Cyanobacteria has been indicated [2,3].…”

Section: Introductionmentioning

confidence: 99%

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The effect of Ca²⁺ on oxygen evolution in membrane preparations from Anacystis nidulans

Brand¹

1979

FEBS Letters

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Section: Discussioncontrasting

confidence: 99%

Section: Discussionmentioning

confidence: 90%

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The effect of Ca²⁺ on oxygen evolution in membrane preparations from Anacystis nidulans

Brand¹

1979

FEBS Letters

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“…While both Mg2e and Ca2+ stimulated turnover rates of PSII reaction centers in Phormidium membranes, Ca2" alone stabilized a maximum number of active reaction centers (26). The presence of Ca2+ during cell disruption was responsible for very high rates of PSII activity in membranes from Anacystis (6).…”

mentioning

confidence: 95%

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca²⁺ or Na⁺

Becker¹,

Brand²

1985

Plant Physiol.

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ABSTRACITAnacystis nidulans exhibits a total loss of photosystem II (PSII) activity upon incubation in a nutrient medium deficient in Ca2' and Na' and containing a divalent cation chelator. This loss of activity is lightdependent, which corresponds to an energy requirement. Likewise, Ca2' efflux takes place only in cells incubated in light. The loss of PSII activity is reversible by addition of submillimolar amounts of either Ca2' or Na' to the external medium but not by the addition of any other cation. Restoration of lost PSII activity also requires light. Light saturation curves for partially depleted cells demonstrate both lower maximum 02 evolution rates and decreased relative quantum yields when compared to control cells. Partial electron transport reactions isolate the site of the Ca2'/Na' effect to the reaction center itself or immediately on its oxidizing side and exclude the water-splitting complex. 02 flash yields decline during cation depletion, indicating a decrease in the number of functional PSII reaction centers, but the maximum turnover rate for still functional reaction centers does not decline. Thus, PSII of A. nidulans exhibits an all-or-none cation requirement, satisfied only by Ca2 or Na'.Early preparations of photosynthetic membranes from cyanobacteria required divalent cations for maximal activity (12,31 (7). We report here a full description of conditions necessary for the reversible loss of PSII activity in intact Anacystis, including the startling finding that Na+ substitutes for Ca2" in this system. We also demonstrate that the site of action for these cations is very near the reaction center. A summary of portions of this work has been presented at a symposium (4). MATERIALS AND METHODSCell Growth and Incubation Conditions. Cg-10 medium was prepared in 3-L batches from stock solutions as described in (32) except that the Fe-EDTA stock solution was prepared from the free acid form of EDTA and the final glycylycine concentration was 11 mm. Acid-washed (0.2 N HCI) glassware was used throughout to minimize cation contamination and the medium was sterilized by autoclaving prior to use. The pH was adjusted to 8.3 with KOH.Anacystis (UTEX 625, Collection of Algae, University of Texas, Austin) was grown at 39C in continuous culture in nutrient medium (28). Cell suspensions of approximately 2 x 108 cells ml-' were aerated continuously with 1.2% CO2 in air and illuminated at approximately 50 uE m-2 s-' PAR with F20T12-CW fluorescent lamps. Cells were harvested, pelleted at 2000g for 10 min, then resuspended to the original cell density in Cg-10 medium devoid of Ca2+. The suspension was pelleted a second time and resuspended in a small volume ofCa2`deficient

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“…Both cyanobacterial photosystems (PSI and PSII) require Ca2+ (England & Evans, 1983;Becker & Brand, 1985;Piccioni & Mauzerall, 1978;Satoh & Katoh, 1985). The activity of PSII in A. nidulans is decreased in parallel with cellular Ca2+ in response to increased incident irradiance (England & Evans, et al, 1983;Becker 8z Brand, 1985).…”

Section: Cyanobacterial Catmodulinsmentioning

confidence: 99%

Calmodulin and calcium mediated regulation in prokaryotes

Onek

Smith

1992

Journal of General Microbiology

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Calcium and photosynthetic oxygen evolution in cyanobacteria

Cited by 58 publications

References 23 publications

The effect of Ca²⁺ on oxygen evolution in membrane preparations from Anacystis nidulans

The effect of Ca²⁺ on oxygen evolution in membrane preparations from Anacystis nidulans

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca²⁺ or Na⁺

Calmodulin and calcium mediated regulation in prokaryotes

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Calcium and photosynthetic oxygen evolution in cyanobacteria

Cited by 58 publications

References 23 publications

The effect of Ca2+ on oxygen evolution in membrane preparations from Anacystis nidulans

The effect of Ca2+ on oxygen evolution in membrane preparations from Anacystis nidulans

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca2+ or Na+

Calmodulin and calcium mediated regulation in prokaryotes

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Product

Resources

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The effect of Ca²⁺ on oxygen evolution in membrane preparations from Anacystis nidulans

The effect of Ca²⁺ on oxygen evolution in membrane preparations from Anacystis nidulans

Anacystis nidulans Demonstrates a Photosystem II Cation Requirement Satisfied Only by Ca²⁺ or Na⁺