Calcium and vitamin D enriched diets increase and preserve vertebral mineral content in aging laboratory rats

Yamaguchi²

2007

Int J Mol Med

Section: Introductionmentioning

confidence: 99%

Genistein and zinc synergistically enhance gene expression and mineralization in osteoblastic MC3T3-E1 cells

Yamaguchi²

2007

Int J Mol Med

“…Bone loss with aging induces osteoporosis, which is widely recognized as a major public health problem (1)(2)(3). The decrease in bone mass leads to bone fracture.…”

Section: Introductionmentioning

confidence: 99%

Oral administration of β-cryptoxanthin prevents bone loss in ovariectomized rats

Yamaguchi²

2006

Int J Mol Med

Abstract. The effect of ß-cryptoxanthin, a kind of carotenoid, on ovariectomy-induced bone loss was investigated. ß-Cryptoxanthin was isolated from Satsuma mandarin (Citrus unshu. MARC). ß-cryptoxanthin (5 or 10 μg/100 g body weight) was orally administered once daily for 3 months to ovariectomized (OVX) rats. OVX induced a significant increase in body weight and a significant decrease in serum calcium and inorganic phosphorus concentrations as compared with those of shamoperated (control) rats. These alterations induced by OVX were significantly prevented by the administration of ß-cryptoxanthin (5 or 10 μg/100 g). The analysis using a peripheral quantitative computed tomography (pQCT) showed that OVX induced a significant decrease in mineral content and mineral density in the femoral-diaphyseal and -metaphyseal tissues and polar strength strain index in the metaphyseal tissues. These decreases were significantly prevented by the administration of ß-cryptoxanthin (5 or 10 μg/100 g). Moreover, OVX induced a significant decrease in calcium content and alkaline phosphatase activity in the femoraldiaphyseal and -metaphyseal tissues and deoxyribonucleic acid (DNA) content in the metaphyseal tissues. These decreases were significantly prevented by the administration of ß-cryptoxanthin (5 or 10 μg/100 g). This study demonstrates that ß-cryptoxanthin has a preventive effect on OVX-induced bone loss in vivo.

“…Cal K 2 The suspensions of Cal K 2 (10, 25, or 50 mg/ 100 g body weight) were orally administered to rats once daily for 7 days, and the animals were killed 24 hr after the last administration. The body weight and serum inorganic phosphorus concentration did not change significantly after Cal K 2 administration ( Table 2).…”

Section: Change In Serum Findings In Rats Administeredmentioning

confidence: 99%

“…The most dramatic expresstion of the disease is represented by fractures of the proximal femurs. [1][2][3][4] A decreases in bone mass may be due to decreased osteoblastic bone formation and increased osteoclastic bone resorption. Pharmacologic and nutritional factors may prevent bone loss with aging.…”

Section: Introductionmentioning

confidence: 99%

Oral Administration of Cal K2 Containing Menaquinone-4 (Vitamin K2) Enhances Serum r-Carboxylated Osteocalcin and Biochemical Components in the Femoral Tissues of Rats

JOURNAL OF HEALTH SCIENCE

Kimura

Masuda

et al. 2006

The effects of menaquinone-4 (MK-4; vitamin K 2 ) supplemented egg shell calcium (Cal K 2 ) on bone components in the femoral-diaphyseal (cortical bone) and -metaphyseal (trabecular bone) tissues of rats was investigated. Cal K 2 contained calcium carbonate (Ca 100 mg/g) and MK-4 (593 µ µ µ µ µg/g). Rats were orally administered a solution of Cal K 2 (10, 25, or 50 mg/ml/100 g body weight) or placebo (without MK-4, 50 mg/ml/100 g body weight) once daily for 7 days. The serum r-carboxylated osteocalcin concentration, which is produced in osteoblastic cells, was significantly increased after the administration of Cal K 2 (50 mg/100 g body weight) as compared with that in the control group or placebocontrol group without MK-4. Calcium content and alkaline phosphatase activity in the femoral-diaphyseal tissues were significantly increased after Cal K 2 (25 or 50 mg/100 g) as compared with that in the placebocontrol group. Femoral-metaphyseal calcium content was significantly increased after the administration of Cal K 2 (25 or 50 mg/100 g) as compared with that in the control group. DNA content in the femoral-diaphyseal and -metaphyseal tissues was significantly increased after the administration of Cal K 2 (50 mg/100 g) as compared with that in the control or placebocontrolled group. This study demonstrates that the oral administration of Cal K 2 containing MK-4 has anabolic effects on bone components in rats. Supplemental Cal K 2 may have a role in the prevention of bone loss with aging.