Calcium and ZmCCaMK are involved in brassinosteroid-induced antioxidant defense in maize leaves

Yan, Jingwei; Guan, Li; Sun, Yi; Zhu, Yuan; Liu, Lei; Lu, Rui; Jiang, Mingyi; Ming, Tan; Zhang, Aying

doi:10.1093/pcp/pcv014

Cited by 68 publications

(24 citation statements)

References 63 publications

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“…Cytosolic calcium concentration was measured by the calcium fluorescent probe Fluo-3/AM (Molecular Probes) based on the method of Yan et al ( 2015 ) and Zhang et al ( 1998 ). Briefly, 1 mM Fluo-3/AM in anhydrous DMSO (5 μL) was added to radish mesophyll protoplasts.…”

Section: Methodsmentioning

confidence: 99%

Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls

et al. 2018

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Our previous studies showed that hydrogen-rich water (HRW) promoted the biosynthesis of anthocyanin under UV-A in radish. However, molecular mechanism involved in the regulation of the anthocyanin biosynthesis is still unclear. In this study, the role of calcium (Ca2+) in HRW-promoted anthocyanin biosynthesis in radish sprouts hypocotyls under UV-A was investigated. The results showed that a positive effect of HRW on the content of cytosolic calcium and anthocyanin accumulation, mimicking the effects of induced CaCl2. Exogenous addition of Ca2+ chelator bis (β-aminoethylether)-N,N,N′,N′-tetraacetic acid (EGTA) and inositol 1,4,5-trisphosphate (IP3) synthesis inhibitor neomycin partially reversed the facilitated effect of HRW. The positive effects of HRW on activity of anthocyanin biosynthetic-enzymes (L-phenylalanine ammonia-lyase, PAL; chalcone isomerase, CHI; dihydroflavonol 4-reductase, DFR and UDP glc-flavonoid 3-O-glucosyl transferase, UFGT) were reversed by EGTA and neomycin. Further tests confirmed that the upregulation of anthocyanin biosynthetic related genes induced by HRW was substantially inhibited by calcium antagonists. The possible involvement of CaM in HRW-regulated anthocyanin biosynthesis was also preliminarily investigated in this study. Taken together, our results indicate that IP3-dependent calcium signaling pathway might be involved in HRW-regulated anthocyanin biosynthesis under UV-A irradiation.

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Section: Methodsmentioning

confidence: 99%

Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls

et al. 2018

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“…Further study founded that a 65 kDa microtubule-associated protein (MAP65), ZmMAP65-1a, directly phosphorylated by ZmMPK5, is required for BR-induced antioxidant defense ( Zhu et al, 2013b ). Recently, Ca 2+ and maize CCaMK gene, ZmCCaMK , was demonstrated to be required for BR-induced antioxidant defense ( Yan et al, 2015 ).…”

Section: Regulation Of Antioxidative System In Crop Plantsmentioning

confidence: 99%

ROS Regulation During Abiotic Stress Responses in Crop Plants

2015

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Abiotic stresses such as drought, cold, salt and heat cause reduction of plant growth and loss of crop yield worldwide. Reactive oxygen species (ROS) including hydrogen peroxide (H2O2), superoxide anions (O2•-), hydroxyl radical (OH•) and singlet oxygen (1O2) are by-products of physiological metabolisms, and are precisely controlled by enzymatic and non-enzymatic antioxidant defense systems. ROS are significantly accumulated under abiotic stress conditions, which cause oxidative damage and eventually resulting in cell death. Recently, ROS have been also recognized as key players in the complex signaling network of plants stress responses. The involvement of ROS in signal transduction implies that there must be coordinated function of regulation networks to maintain ROS at non-toxic levels in a delicate balancing act between ROS production, involving ROS generating enzymes and the unavoidable production of ROS during basic cellular metabolism, and ROS-scavenging pathways. Increasing evidence showed that ROS play crucial roles in abiotic stress responses of crop plants for the activation of stress-response and defense pathways. More importantly, manipulating ROS levels provides an opportunity to enhance stress tolerances of crop plants under a variety of unfavorable environmental conditions. This review presents an overview of current knowledge about homeostasis regulation of ROS in crop plants. In particular, we summarize the essential proteins that are involved in abiotic stress tolerance of crop plants through ROS regulation. Finally, the challenges toward the improvement of abiotic stress tolerance through ROS regulation in crops are discussed.

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“…In a recent study, BR treatment was unable to elicit antioxidant defense in the rice and maize CALCIUM/CALMODULIN-DEPENDENT PROTEIN KINASE (CCaMK) mutants. It was found that BR application results in increase in cytosolic Ca (2+) concentration followed by increase in activity of CCaMK which further enhanced the BR-induced increase in cytosolic Ca (2+) concentration thus forming a positive feedback loop of Ca (2+) and CCaMK in BR signaling ( Yan et al, 2015 ). ABA has also been demonstrated to be intricately involved in BR-orchestrated antioxidant defense via ZmMAP65-1a as inhibition of ABA biosynthesis reduces the expression and activity of ZmMAP65-1a ( Zhu et al, 2015 ).…”

Section: Role Of Brs At Physiological and Biochemical Levelmentioning

confidence: 99%

Brassinosteroids: A Promising Option in Deciphering Remedial Strategies for Abiotic Stress Tolerance in Rice

2017

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Rice is an important staple crop as it feeds about a half of the earth’s population. It is known to be sensitive to a range of abiotic stresses which result in significant decline in crop productivity. Recently, the use of phytohormones for abiotic stress amelioration has generated considerable interest. Plants adapt to various environmental stresses by undergoing series of changes at physiological and molecular levels which are cooperatively modulated by various phytohormones. Brassinosteroids (BRs) are a class of naturally occurring steroidal phytohormones, best known for their role in plant growth and development. For the past two decades, greater emphasis on studies related to BRs biosynthesis, distribution and signaling has resulted in better understanding of BRs function. Recent advances in the use of contemporary genetic, biochemical and proteomic tools, with a vast array of accessible biological resources has led to an extensive exploration of the key regulatory components in BR signaling networks, thus making it one of the most well-studied hormonal pathways in plants. The present review highlights the advancements of knowledge in BR research and links it with its growing potential in abiotic stress management for important crop like rice.

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Calcium and ZmCCaMK are involved in brassinosteroid-induced antioxidant defense in maize leaves

Cited by 68 publications

References 63 publications

Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls

Increased Cytosolic Calcium Contributes to Hydrogen-Rich Water-Promoted Anthocyanin Biosynthesis Under UV-A Irradiation in Radish Sprouts Hypocotyls

ROS Regulation During Abiotic Stress Responses in Crop Plants

Brassinosteroids: A Promising Option in Deciphering Remedial Strategies for Abiotic Stress Tolerance in Rice

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