Calcium as a Coagonist of Inositol 1,4,5-Trisphosphate-Induced Calcium Release

Finch, Elizabeth A.; Turner, Timothy J.; Goldin, Stanley M.

doi:10.1126/science.2017683

Cited by 821 publications

(616 citation statements)

References 33 publications

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“…This discrepancy could be explained by taking into account that calcium channel modulators produced a bell-shaped curve of modulation of the mobility time. This biphasic effect was similar to the effect produced by the physiological modulators of InsP 3 R and RyR (Finch et al, 1991;Copello et al, 1997).…”

Section: Discussionsupporting

confidence: 59%

Blockade of intracellular calcium release induces an antidepressant-like effect in the mouse forced swimming test

2006

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The role of intracellular calcium in the modulation of a depressant-like condition was investigated in the mouse forced swimming test. I.c.v. administration of TMB-8 (0.23e46.3 nmol per mouse), a blocker of Ca 2C release from intracellular stores, decreased the mouse immobility time. I.c.v. injection of thapsigargin (0.003e3 nmol per mouse), compound which selectively inhibits Ca 2C uptake into the endoplasmic reticulum, produced, 60 min after administration, a depressant-like condition. Xestospongin C (1e100 pmol per mouse i.c.v.), an InsP 3 -receptor antagonist, decreased the mouse immobility time. By contrast, D-myo-inositol (5.4e540 pmol per mouse i.c.v.), compound which produces InsP 3 , resulted in a depressant-like effect. Similarly, ryanodine (0.1e600 pmol per mouse i.c.v.), an RyR antagonist, decreased the immobility time values whereas the administration of 4-chloro-m-cresol (0.1e100 pmol per mouse i.c.v.), an RyR agonist, showed an opposite effect. The antidepressant-like effects observed with TMB-8, xestospongin C and ryanodine were comparable to that produced by the antidepressant drugs amitriptyline and clomipramine. The treatments employed did not produce any behavioural impairment of mice as revealed by the rota-rod and hole board tests indicating that the antidepressant-and depressant-like effects were not due to a compromised locomotor activity and spontaneous motility of the treated animals. These results indicate that a central variation in intracellular calcium contents is involved in the modulation of a depressive-like condition in the mouse forced swimming test. In particular, the blockade of both InsP 3 Rs and RyRs appears to play an important role in the induction of an antidepressant-like effect, whereas the stimulation of these receptors is involved in a depressant-like response of mice.

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Section: Discussionsupporting

confidence: 59%

Blockade of intracellular calcium release induces an antidepressant-like effect in the mouse forced swimming test

2006

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“…β-mercaptoethanol was from Serva. 3 H-InsP 3 was from Dupont NEN. Triton X-100 (TX-100) was from FARCO.…”

Section: Methodsmentioning

confidence: 99%

“…Much different from the phys iological condition, the InsP 3 binding was assayed at 0 4 in all published works up to now, which may be unfavorable to observing the effects of some factors on InsP 3 receptor. Therefore, the activity of purified InsP 3 receptor binding to 3 3 receptor from mouse cerebellum disappeared after purification [4] . Twenty one tryptophane residues can be found in the mouse cerebellar InsP 3 receptor, sixteen of which were located in cytosolic domain and five in transmembrane domain [13] .…”

Section: Purification Of Insp 3 Receptormentioning

confidence: 99%

“…The regulation of inositol 1, 4, 5-trisphosphate (InsP 3 ) receptor by Ca 2+ was very complex according to current studies. Most results obtained in cells and microsomes showed that the InsP 3 receptor was activated in the lower concentration range (200 500 nmol/L) of cytoplasmic Ca 2+ and was strongly inhibited when the Ca 2+ concentration exceeded 1 µmol/L [2,3] . Fewer studies on regulation of purified InsP 3 receptor by Ca 2+ could be found in literatures and the results were controversial [4,5] .…”

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confidence: 99%

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Temperature-dependence and conformational basis of inositol 1,4,5-trisphosphate receptor regulated by Ca2+

Huang

Yang

2000

Sci. China Ser. C.-Life Sci.

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The inositol 1,4,5-trisphosphate (InsP 3 ) receptor was purified from bovine cerebellum and reconstituted in liposomes composed of phosphatidylcholine (PC) and phosphatidylethanolamine (PE) Inositol 1,4,5-trisphosphate receptor, as an indispensable member in the inositol phosphates signal transduction across membrane, played an important role in Ca 2+ release from endoplasmic reticulum (ER) and subsequently complicated cellular Ca 2+ signal such as Ca 2+ spike and Ca 2+ oscillation [1] . The regulation of inositol 1, 4, 5-trisphosphate (InsP 3 ) receptor by Ca 2+ was very complex according to current studies. Most results obtained in cells and microsomes showed that the InsP 3 receptor was activated in the lower concentration range (200 500 nmol/L) of cytoplasmic Ca 2+ and was strongly inhibited when the Ca 2+ concentration exceeded 1 µmol/L [2,3] . Fewer studies on regulation of purified InsP 3 receptor by Ca 2+ could be found in literatures and the results were controversial [4,5] . The effects of Ca 2+ on the InsP 3 binding were examined carefully after purific ation and reconstitution of InsP 3 receptor, and the intrinsic fluorescence was further measured to study the underlying conformational basis.

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“…Increasing the cytosolic Ca# + concentration results in a bell-shaped dose-response curve for InsP $ -induced Ca# + release (IICR) or channel activity [9]. The activating and inhibiting effects of Ca# + are rapid processes, developing within 1 s [10,11]. In several tissues, including the cerebellum, InsP $ , in addition to opening the Ca# + channel, triggers a slower effect leading to the conversion of its Abbreviations used : IICR, Ins(1,4,5)P 3 -induced Ca 2 + release ; InsP 3 , Ins(1,4,5)P 3 ; InsP 3 R, Ins(1,4,5)P 3 receptor ; NTA, nitrilotriacetic acid.…”

Section: Introductionmentioning

confidence: 99%

Regulation of cerebellar Ins(1,4,5)P3 receptor by interaction between Ins(1,4,5)P3 and Ca2+

Coquil

Picard

Mauger

1999

Biochemical Journal

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We have characterized in detail the Ca# + -dependent inhibition of [$H]Ins(1,4,5)P $ ([$H]InsP $ ) binding to sheep cerebellar microsomes, over a short duration (3 s), with the use of a perfusion protocol. This procedure prevented artifacts previously identified in studies of this Ca# + effect. In a cytosol-like medium at pH 7.1 and 20 mC, a maximal inhibition of approx. 50 % was measured. Both inhibition and its reversal were complete within 3 s. Ca# + decreased the affinity of the receptor for InsP $ by approx. 50 % (K d 146p24 nM at pCa 9 and 321p56 nM at pCa 5.3), without changing the total number of binding sites. Conversely, increasing the [$H]InsP $ concentration from 30 to 400 nM tripled the IC &! for Ca# + and decreased the maximal inhibition by 63 %. This is similar to a partial competitive inhibition between InsP $ binding and inhibitory Ca# + binding and is consistent with InsP $ and

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Calcium as a Coagonist of Inositol 1,4,5-Trisphosphate-Induced Calcium Release

Cited by 821 publications

References 33 publications

Blockade of intracellular calcium release induces an antidepressant-like effect in the mouse forced swimming test

Blockade of intracellular calcium release induces an antidepressant-like effect in the mouse forced swimming test

Temperature-dependence and conformational basis of inositol 1,4,5-trisphosphate receptor regulated by Ca2+

Regulation of cerebellar Ins(1,4,5)P3 receptor by interaction between Ins(1,4,5)P3 and Ca2+

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