Calcium carbonate films and helices grown in solutions of poly(aspartate)

Gower, Laurie B.; Tirrell, David A.

doi:10.1016/s0022-0248(98)00002-5

Cited by 332 publications

(343 citation statements)

References 16 publications

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“…The influence of organic matter, exopolysaccharides and polyaspartate in the precipitation of vaterite can also be found in nature (Falini et al 1998;Gower and Tirrell 1998). Vaterite crystals similar to those obtained in this study have recently been found in Lake Issyk-Kul (Kyrgyzstan) and have been attributed to Synechococcus activity in mucilagenous mats containing polyaspartate (Giralt et al 2001).…”

Section: Discussionsupporting

confidence: 82%

Is the contribution of bacteria to terrestrial carbon budget greatly underestimated?

Braissant

Verrecchia

Aragno

2002

Naturwissenschaften

119

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Some commonly found species of soil bacteria use low molecular weight organic acids as their sole source of carbon and energy. This study shows that acids such as citrate and oxalate (produced in large amounts by fungi and plants) can rapidly be consumed by these bacteria. Two strains, Ralstonia eutropha and Xanthobacter autotrophicus, were cultured on acetate-and citrate-rich media. The resulting CO 2 and/or HCO 3 -reacted with calcium ions to precipitate two polymorphs of calcium carbonate (CaCO 3 ), calcite and vaterite, depending on the quantity of slime produced by the strains. This production of primary calcium carbonate crystals by oxalate-and citrate-degrading bacteria from soil organic carbon sources highlights the existence of an important and underestimated potential carbon sink.

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Section: Discussionsupporting

confidence: 82%

Is the contribution of bacteria to terrestrial carbon budget greatly underestimated?

Braissant

Verrecchia

Aragno

2002

Naturwissenschaften

119

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“…The formation of spiral pits at lower concentration of anso- calcin (0.1 g/ml) indicates strong interaction between the acidic groups present in the protein and the growing crystal nuclei. Similar effects were observed in the presence of highly acidic polyaspartic acid molecules (53). As the concentration of ansocalcin was increased (Ͼ50 g/ml), calcite crystal aggregates were formed.…”

Section: Discussionsupporting

confidence: 71%

Purification, Characterization, and in VitroMineralization Studies of a Novel Goose Eggshell Matrix Protein, Ansocalcin

Lakshminarayanan

Valiyaveettil

Rao

et al. 2003

Journal of Biological Chemistry

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Biomineralization is an important process in which hard tissues are generated through mineral deposition, often assisted by biomacromolecules. Eggshells, because of their rapid formation via mineralization, are chosen as a model for understanding the fundamentals of biomineralization. This report discusses purification and characterization of various proteins and peptides from goose eggshell matrix. A novel 15-kDa protein (ansocalcin) was extracted from the eggshell matrix, purified, and identified and its role in mineralization evaluated using in vitro crystal growth experiments. The complete amino acid sequence of ansocalcin showed high homology to ovocleidin-17, a chicken eggshell protein, and to C-type lectins from snake venom. The amino acid sequence of ansocalcin was characterized by the presence of acidic and basic amino acid multiplets. In vitro crystallization experiments showed that ansocalcin induced pits on the rhombohedral faces at lower concentrations (<50 g/ml). At higher concentrations, the nucleation of calcite crystal aggregates was observed. Molecular weight determinations by size exclusion chromatography and sodium dodecyl sulfate -polyacrylamide gel electrophoresis showed reversible concentrationdependent aggregation of ansocalcin in solution. We propose that such aggregated structures may act as a template for the nucleation of calcite crystal aggregates. Similar aggregation of calcite crystals was also observed when crystallizations were performed in the presence of whole goose eggshell extract. These results show that ansocalcin plays a significant role in goose eggshell calcification.Organisms are capable of developing minerals and biocomposites with complex architecture to fulfill important biological functions, such as skeletal support, protection of soft tissues, and food grinding (1-4). Often, survival of the organism depends on the structure and strength of these composite materials. Due to the wide range of mechanical and functional properties of these biocomposites, identifying the organic and inorganic components and understanding the structure-function relationships have potential industrial and biotechnological applications (5). The inorganic mineral phase of such materials is formed over an insoluble organic matrix or mold, and the mineral phase is intimately associated with organic macromolecules, such as proteins/glycoproteins, polysaccharides, or proteoglycans (6). These biomacromolecules are highly acidic in nature and have been postulated to control nucleation, growth, crystal size, and shape of the mineral phases (7).Mann (8) classified the biologically programmed composites into four types (types I, II, III, and IV), based on matrix intervention in nucleation and growth processes. Avian eggshells form type II biocomposites, which is the fastest forming hard acellular composite in nature. For example, in the case of chicken eggshell, about 5 g of the mineral phase is produced within 24 h (9). Their calcified layer consists of ϳ95% mineral and ϳ5% organic phase (10). The ...

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“…Such actions are believed to be related to their functional groups, including SO 4 2-, PO 4 3-and COO - [1,2]. Some natural amino acids, including sodium salts of poly(aspartic acid) and poly(glutamic acid), have been reported to influence both calcium carbonate and calcium phosphate crystal growth [3][4][5][6][7]. In contrast to less branched polypeptides, dendritic molecules, for example, poly(amidoamine) (PAMAM) dendrimers have attracted increasing interest for the regulation of inorganic compounds [8,9] due to their controllable sizes, well-defined structures, and multiple functional groups.…”

Section: Introductionmentioning

confidence: 99%

Effects of glutamic acid shelled PAMAM dendrimers on the crystallization of calcium phosphate in diffusion systems

et al. 2010

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Second generation poly(amidoamine) (PAMAM) dendrimers were synthesized and peripherally modified with glutamic acid (PAMAM-MG) as a shell. The effect of the dendrimers on the crystallization of different calcium phosphate compounds was investigated in both double and one way diffusion systems. It was found that the crystals of calcium phosphate showed tape-like morphology in the presence of PAMAM-MG, and the crystals' thickness and width decreased compared to those grown without dendritic molecules. Such a result might be due to the interaction of electric charges between dendritic molecules and octacalcium phosphate (Ca 8 H 2 (PO 4 ) 6 Á5H 2 O, OCP), which led to the adsorption of PAMAM-MG in the 100 and 010 surfaces of OCP. Moreover, PAMAM-MG showed an affinity for gelatin, and it could cause the formation of amorphous calcium phosphate (Ca 9 (PO 4 ) 6 ÁnH 2 O, ACP) at a concentration of 5 mg/mL of PAMAM-MG. These results suggest that PAMAM-MG could be used for regulating the morphology of OCP and changing the composition of minerals in gels.

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Calcium carbonate films and helices grown in solutions of poly(aspartate)

Cited by 332 publications

References 16 publications

Is the contribution of bacteria to terrestrial carbon budget greatly underestimated?

Is the contribution of bacteria to terrestrial carbon budget greatly underestimated?

Purification, Characterization, and in VitroMineralization Studies of a Novel Goose Eggshell Matrix Protein, Ansocalcin

Effects of glutamic acid shelled PAMAM dendrimers on the crystallization of calcium phosphate in diffusion systems

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