Calcium Carbonate Nanoparticles Stimulate Tumor Metabolic Reprogramming and Modulate Tumor Metastasis

Abstract

CaCO 3 nanoparticles (nano-CaCO 3 ) can neutralize the acidic pHe of solid tumors, but the lack of intrinsic imaging signal precludes noninvasive monitoring of pH-perturbation in tumor microenvironment. We aim to develop a theranostic version of nano-CaCO 3 to noninvasively monitor pH modulation and subsequent tumor response. Materials & methods: We synthesized ferromagnetic core coated with CaCO 3 (magnetite CaCO 3 ). Magnetic resonance imaging (MRI) was used to determine the biodistribution and pH modulation using murine fibrosarcoma and breast cancer models. Results: Magnetite CaCO 3 -MRI imaging showed that nano-CaCO 3 rapidly raised tumor pHe, followed by excessive tumor-associated acid production after its clearance. Continuous nano-CaCO 3 infusion could inhibit metastasis. Conclusion: Nano-CaCO 3 exposure induces tumor metabolic reprogramming that could account for the failure of previous intermittent pH-modulation strategies to achieve sustainable therapeutic effect.

Cellular Investigations on Mechanistic Biocompatibility of Green Synthesized Calcium Oxide Nanoparticles with Danio rerio

“…Calcium oxide nanoparticles are used as reliable nanoparticles for biomedical purposes [11]. They are effective against microorganisms, such as Streptococcus aureus, Bacillus cereus, Escherichia coli, and Pseudomonas aeruginosa [7].…”

Eco-friendly processes for the synthesis of amorphous calcium carbonate nanoparticles in ethanol and their stabilisation in aqueous media

“…The synthesis in ethanol limits the presence of water, preventing the spontaneous aggregation and crystallisation of CaCO 3 NPs without the need for synthetic additives. 6,11,20,21,23,26,45–50 This method leads to the obtention of pure ACC nanoparticles with a size under 150 nm.…”

Synthesis and Characterization of Gefitinib and Paclitaxel Mono and Dual Drug-Loaded Blood Cockle Shells (Anadara granosa)-Derived Aragonite CaCO3 Nanoparticles

“…Calcium carbonate nanoparticles have been synthesized by both top-down [ 35 , 45 , 49 ] and bottom-up methods [ 50 , 51 ]. Each method has its advantages and disadvantages.…”