Calcium Carbonate Particle Growth Depending on Coupling among Adjacent Layers in Hybrid LB/LbL Films

Ramos, Ana Paula; Nobre, Thatyane M.; Montoro, Luciano A.; Zaniquelli, Maria Elisabete Darbello

doi:10.1021/jp8023793

Cited by 12 publications

(10 citation statements)

References 63 publications

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“…Ca 2+ -containing subphases diminish the minimum molecular area of DMPA monolayers Figure 3 shows the π-A isotherms of DMPA. The shape of the isotherm is in agreement with previously reported data [40,41]. The compression of the monolayer was observed in the presence of CaCl 2 at the subphase.…”

Section: Resultssupporting

confidence: 91%

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Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?

Andrade

Derradi

Simão

et al. 2019

Archives of Biochemistry and Biophysics

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Tissue-nonspecific alkaline phosphatase (TNAP) is a key enzyme in the biomineralization process as it produces phosphate from a number of phospho-substrates stimulating mineralization while it also inactivates inorganic pyrophosphate, a potent mineralization inhibitor. We have previously reported on the reconstitution of TNAP on Langmuir monolayers as well as proteoliposomes. In the present study, thin films composed of dimyristoylphosphatidic acid (DMPA) were deposited on titanium supports by the Langmuir-Blodgett (LB) technique, and we determined preservation of TNAP's phosphohydrolytic activity after incorporation into the LB films. Increased mineralization was observed after exposing the supports containing the DMPA:TNAP LB films to solutions of phospho-substrates, thus evidencing the role of TNAP on the growth of calcium phosphates after immobilization. These coatings deposited on metallic supports can be potentially applied as osteoconductive materials, aiming at the optimization of bone-substitutes integration in vivo.

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Section: Resultssupporting

confidence: 91%

“…The salt concentration used in this study was higher than the concentrations generally used for DMPA Langmuir monolayers [41,42]. This high ionic-strength was used in order to coadsorb TNAP with DMPA (the first present at the interface only through the salting-out effect).…”

Section: Ca 2+ -Containing Subphases Enhance the Amount Of Dmpa Transmentioning

confidence: 99%

Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?

Andrade

Derradi

Simão

et al. 2019

Archives of Biochemistry and Biophysics

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“…(5) Miñones, J., Jr.; Pais, S.; Miñones, J.; Conde, O.; Dynarowicz-ya -tka, P. Biophys. Chem but also the layer-by-layer (LbL) technique [18][19][20] or a combination of both LB and LbL techniques 21,22 have been explored in the fabrication of supramolecular architectures with molecular level control. Dipalmitoylphosphatidylglycerol (DPPG) is a phospholipid extensively applied in studies involving mimetic systems in the form of Langmuir monolayers, 7,13,16,[23][24][25] LB films, 13,16,[24][25][26] unilamellar vesicles, 7,26,27 and multilamellar vesicles.…”

Section: Introductionmentioning

confidence: 99%

Taking Advantage of Electrostatic Interactions To Grow Langmuir−Blodgett Films Containing Multilayers of the Phospholipid Dipalmitoylphosphatidylglycerol

et al. 2009

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The use of phospholipids as mimetic systems for studies involving the cell membrane is a well-known approach. In this context, the Langmuir and Langmuir-Blodgett (LB) methods are among the main techniques used to produce ordered layers of phospholipids structured as mono- or bilayers on water subphase and solid substrates. However, the difficulties of producing multilayer LB films of phospholipids restrict the application of this technique depending on the sensitivity of the experimental analysis to be conducted. Here, an alternative approach is used to produce LB films containing multilayers of the negative phospholipid dipalmitoylphosphatidylglycerol (DPPG). Inspired by the electrostatic layer-by-layer (LbL) technique, DPPG multilayer LB films were produced by transferring the DPPG Langmuir monolayers from the water subphase containing low concentrations of the cationic polyelectrolyte poly(allylamine hydrochloride) (PAH) onto solid substrates. Fourier transform infrared (FTIR) absorption spectroscopy revealed that the interactions between the NH(3)(+) (PAH) and PO(4)(-) (DPPG) groups might be the main driving forces that allow growth of these LB films. Besides, ultraviolet-visible (UV-vis) absorption spectroscopy showed that the multilayer LB films can be grown in a controlled way in terms of thickness at nanometer scale. Cyclic voltammetry showed that DPPG and PAH are more packed in the LB than LbL films. The latter finding is related to the distinct molecular architecture of the films since DPPG is structured as monolayers in the LB films and multilamellar vesicles in the LbL films. Despite the interaction with PAH, cyclic voltammetry also showed that DPPG retains its biological activity in LB films, which is a key factor since this makes DPPG a suitable material in sensing applications. Therefore, multilayer LB films were deposited onto Pt interdigitated electrodes forming sensing units, which were applied in the detection of a phenothiazine compound [methylene blue (MB)] using impedance spectroscopy. The performance of DPPG in single-layer and multilayer LB films was compared to the performance of sensing unities composed of DPPG in single-layer and multilayer LbL films, showing the importance of both the thickness and the molecular architecture of the thin films. As found in a previous work for LbL films, the high sensitivity reached by these sensing units is intimately related to changes in the morphology of the film as evidenced by the micro-Raman technique. Finally, the interaction between MB and the (DPPG+PAH) LB films was complemented by pi-A isotherms and surface-enhanced resonance Raman scattering (SERRS).

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“…48 Biomimetic approaches have been used in order to maintain the mechanical properties of the metals simultaneously increasing their bioactivity through surface modifications. [49][50][51][52][53][54][55][56][57] The further adsorption of COL1 to titanium surfaces using biomimetic immobilization into phospholipid Langmuir-Blodgett films stimulated apatite precipitation and proliferation of osteoblasts compared to bare titanium. 58 The materials used for bone regeneration can be found as a permanent graft or a temporary tissue connector.…”

Section: Bone Tissue Engineering: Moving Toward Biomimetic Approachesmentioning

confidence: 99%

Three‐dimensional cell‐laden collagen scaffolds: From biochemistry to bone bioengineering

et al. 2021

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The bones can be viewed as both an organ and a material. As an organ, the bones give structure to the body, facilitate skeletal movement, and provide protection to internal organs. As a material, the bones consist of a hybrid organic/inorganic threedimensional (3D) matrix, composed mainly of collagen, noncollagenous proteins, and a calcium phosphate mineral phase, which is formed and regulated by the orchestrated action of a complex array of cells including chondrocytes, osteoblasts, osteocytes, and osteoclasts. The interactions between cells, proteins, and minerals are essential for the bone functions under physiological loading conditions, trauma, and fractures. The organization of the bone's organic and inorganic phases stands out for its mechanical and biological properties and has inspired materials research. The objective of this review is to fill the gaps between the physical and biological characteristics that must be achieved to fabricate scaffolds for bone tissue engineering with enhanced performance. We describe the organization of bone tissue highlighting the characteristics that have inspired the development of 3D cell-laden collagenous scaffolds aimed at replicating the mechanical and biological properties of bone after implantation. The role of noncollagenous macromolecules in the organization of the collagenous matrix and mineralization ability of entrapped cells has also been reviewed. Understanding the modulation of cell activity by the extracellular matrix will ultimately help to improve the biological performance of 3D cell-laden collagenous scaffolds used for bone regeneration and repair as well as for in vitro studies aimed at unravelling physiological and pathological processes occurring in the bone.

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Calcium Carbonate Particle Growth Depending on Coupling among Adjacent Layers in Hybrid LB/LbL Films

Cited by 12 publications

References 63 publications

Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?

Is alkaline phosphatase biomimeticaly immobilized on titanium able to propagate the biomineralization process?

Taking Advantage of Electrostatic Interactions To Grow Langmuir−Blodgett Films Containing Multilayers of the Phospholipid Dipalmitoylphosphatidylglycerol

Three‐dimensional cell‐laden collagen scaffolds: From biochemistry to bone bioengineering

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