Calcium channel blocker and renal mitochondrial function in warm renal ischemia

Tucci, Sílvio; Borelli-Bovo, Tiago; Cologna, Adauto José; Tiraboschi, Ricardo Brianezi; Martins, Antônio Carlos Pereira; Roselino, J. E.

doi:10.1590/s1677-55382005000400014

Cited by 10 publications

(8 citation statements)

References 21 publications

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“…By preventing the calcium influx, it may help in the decrease of progression of IR injury . It has been detected to protect against renal IR induced in rats …”

Section: Introductionmentioning

confidence: 99%

Study of the protective effect of ischemic and pharmacological preconditioning on hepatic ischemic reperfusion injury induced in rats

Osman

Kamel

2017

JGH Open

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Background and AimHepatic ischemia reperfusion injury is the main cause of liver failure following liver surgery, so an effective method is needed to prevent or reduce this hepatic injury. The aim of the present study is to investigate the potential effect of ischemic preconditioning versus pharmacological preconditioning with lisinopril or verapamil for protection against hepatic ischemia reperfusion injury induced in rats.MethodsRats were divided into six groups. Group I served as control untreated. Rats of group II were subjected to laparotomy without induction of ischemia reperfusion. Ischemia reperfusion by ligation of the portal trait for 30 min, followed by reperfusion for 2 h, was performed in rats of groups III–VI. Ischemic preconditioning was performed for rats of group IV before induction of ischemia reperfusion. Lisinopril and verapamil was given daily for 3 days before induction of ischemia reperfusion in groups V and VI, respectively. Serum level of liver transaminases and liver malondialdehyde content were measured, and hepatic histopathological examination was assessed.ResultsInduction of ischemia reperfusion resulted in significant elevation of liver transaminases and liver malondialdehyde content associated with significant hepatic histopathological injury that were significantly improved by ischemic preconditioning, lisinopril, or verapamil treatment. Verapamil showed the most significant improvement compared with ischemic preconditioning or lisinopril treatment.ConclusionIschemic preconditioning and pharmacological preconditioning by lisinopril or verapamil can protect against hepatic ischemia reperfusion probably through inhibition of oxidative stress and neutrophil infiltration. The most potent protection is demonstrated by verapamil treatment.

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“…By preventing the calcium influx, it may help in the decrease of progression of IR injury . It has been detected to protect against renal IR induced in rats …”

Section: Introductionmentioning

confidence: 99%

Study of the protective effect of ischemic and pharmacological preconditioning on hepatic ischemic reperfusion injury induced in rats

Osman

Kamel

2017

JGH Open

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“…ROS per se have also been shown to compromise renal function, depress glomerular filtration, impair glomerular sieving function [5,6,7], and induce apoptosis in renal cells [8]. A range of drugs and methods have been tested with the intention to block the pathways of cell injury at different levels and thereby enhance viability of the kidney challenged by I-R stress with variable results, such as: ischemic preconditioning [9], antioxidants [10,11], dipyridamole [12,13], nicotine [14], and calcium channel blockers [15,16,17,18]. Recent overwhelming attention to plant products and alternative medicine has encouraged plant chemists, pharmacologists and biochemists to combine their efforts in a search for natural agents, such as Allium sativum , Benincasa cerifa (Thunb.…”

Section: Introductionmentioning

confidence: 99%

Preventive Effect of Ginger <i>(Zingiber officinale)</i> Pretreatment on Renal Ischemia-Reperfusion in Rats

et al. 2010

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Background/Aims: The present study examined the preventive effect of ginger against renal ischemia-reperfusion (I-R) injury. Methods: 30 adultmale rats were used. The animals were allocated to five groups of 6 rats: (1) normal (N), (2) normal + ginger (N+G), (3) right nephrectomy (Sham), (4) I-R, and (5) I-R + ginger (I-R+G). To induce renal ischemia, animals were unilaterally nephrectomized and subjected to 45 min of left renal pedicle occlusion with 24 h reperfusion. Ginger was administered orally 24 h and just before ischemia. At the end of the experiment, blood samples and urine were collected. The following renal functional parameters were studied: serum and urinary levels of creatinine and urea, fractional excretion of sodium, and creatinine clearance. Histopathological examination of the kidney was also performed. Results: Rats with renal I-R showed a significant increase in creatinine and urea concentration in serum and also a significant decrease in creatinine clearance compared with the other groups. In addition, renal histopathology in the I-R group showed severe alterations such as tubular dilatation and tubular epithelial necrosis. However, these changes were significantly reduced in the I-R+G group. Conclusion: This study suggests that ginger is a useful agent for the prevention of renal ischemia reperfusion-induced injury.

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“…3,4 Lipid peroxidation is an autocatalytic mechanism leading to oxidative destruction of cellular membranes, and their destruction can lead to the production of toxic reactive aldehydic metabolites and cell death. [5][6][7][8] A range of drugs and methods have been tested with the intention to block the pathways of cell injury at different levels and to thereby enhance viability of the kidney challenged by ischemia/ reperfusion stress with variable results, such as: heat shock (interferes with the L-arginine-nitric oxide pathway), 9 antioxidants, 10,11 dipyridamole (increases endogenous adenosine), 12,13 nicotine (inhibit cholinergic antiinflammatory pathway), 14 calcium channel blockers or antagonists, [15][16][17][18] chlorpromazine, 19,20 etc. Chorpromazine (CPZ) is a membrane stabilizer and seems to be able to preserve mitochondria integrity and to reduce phospholipids degradation of microsomal membranes of liver cell provoked by ischemia/reperfusion.…”

Section: Introductionmentioning

confidence: 99%

Renal ischemia and reperfusion injury: influence of chorpromazine on renal function and lipid peroxidation

et al. 2008

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Purpose: To evaluate the influence of chlorpromazine (CPZ) on renal function and lipid peroxidation in a rat model of kidney ischemia/reperfusion injury. Methods: Forty eight Wistar rats underwent a laparotomy for hilar clamping of left kidney with a bulldog clamp for 60 minutes followed by organ reperfusion and contralateral nephrectomy. Of these, 26 received 3mg/kg of CPZ intravenously 15 minutes before renal ischemia (G-E) while the remaining 22 were used as ischemic control group (G-C). Eleven rats of G-E and 8 of G-C were followed for blood urea nitrogen and creatinine determinations before renal ischemia and at 1 st , 4 th and 7 th postoperative days. Samplings of left renal tissue were obtained at 5 minutes (5 rats from each group) and 24 hours (9 G-C and 10 of G-E) of reperfusion for malondialdehy (MDA) content determination. Controls of renal MDA content were determined in kidneys harvested from 6 additional normal rats. Results: Acute renal failure occurred in all animals but levels of BUN and creatinine were significantly lower in G-E (p<0.001). MDA content rose strikingly at 5 minutes of reperfusion in both groups (p>0.05) and returned near to normal levels 24 hours later. Conclusion: CPZ conferred partial protection of renal function to kidneys submitted to ischemia/reperfusion injury that seems to be not dependent on inhibition of lipid peroxidation. Key words:Renal Ischemia/Reperfusion. Lipid Peroxidation. Malondialdehyde. Chlorpromazine. Free Radicals. RESUMOObjetivo: avaliar a influência da clorpromazina (CPZ) na função renal e na peroxidação lipídica num modelo de lesão de isquemia/reperfusão renal em ratos. Métodos: 48 ratos Wistar foram submetidos à laparotomia para clampamento da artéria renal esquerda durante 60 minutos, seguido da reperfusão e nefrectomia contralateral. Destes animais, 26 receberam 3 mg/ kg de CPZ intravenosa 15 minutos antes da isquemia renal (G-E), sendo os 22 animais restantes utilizados como grupo controle isquêmico (G-C). Em 11 ratos do G-E e 8 do G-C foi feita a dosagem de uréia e creatinina sérica antes da isquemia renal e no 1º, 4º e 7º dia pós-operatório. Amostras de tecido do rim esquerdo foram obtidas aos 5 minutos (5 ratos de cada grupo) e 24 horas após reperfusão (9 G-C e 10 G-E) para dosagem de malondialdeído (MDA). Valores controle para níveis de MDA foram obtidos em rins retirados de 6 ratos normais. Resultados: insuficiência renal aguda ocorreu em todos os animais mas os níveis séricos de uréia e creatinina foram significativamente menores no G-E (p<0,001). Os níveis de MDA apresentaram elevação acentuada na avaliação aos 5 minutos de reperfusão em ambos os grupos (p<0,05), retornando a valores próximos aos normais na avaliação com 24 horas. Conclusão: a CPZ conferiu proteção parcial da função renal aos rins submetidos à lesão de isquemia e reperfusão, aparentemente independente da inibição da peroxidação lipídica.

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Calcium channel blocker and renal mitochondrial function in warm renal ischemia

Cited by 10 publications

References 21 publications

Study of the protective effect of ischemic and pharmacological preconditioning on hepatic ischemic reperfusion injury induced in rats

Study of the protective effect of ischemic and pharmacological preconditioning on hepatic ischemic reperfusion injury induced in rats

Preventive Effect of Ginger <i>(Zingiber officinale)</i> Pretreatment on Renal Ischemia-Reperfusion in Rats

Renal ischemia and reperfusion injury: influence of chorpromazine on renal function and lipid peroxidation

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