Calcium-dependent action of osmolality on adenosine 3',5'-monophosphate accumulation in rat renal inner medulla: evidence for a relationship to calcium-responsive arachidonate release and prostaglandin synthesis.

Craven, Patricia A.; Briggs, Robert G.; DeRubertis, Frederick R.

doi:10.1172/jci109697

Cited by 57 publications

(32 citation statements)

References 43 publications

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“…Together with the studies indicating that elevation of tissue NaCl concentration stimulates prostaglandin biosynthesis (Dannon et al 1978;Craven et al 1980;Yang et al 1999), the present experiments with frusemide suggest that, in addition to the known effect of medullary blood flow changes on local interstitial hypertonicity (the action of the countercurrent exchanger), changes in hypertonicity can feed back to the medullary vasculature and affect perfusion of the medulla.…”

Section: Discussionsupporting

confidence: 77%

Differential effect of frusemide on renal medullary and cortical blood flow in the anaesthetised rat

Dobrowolski¹,

Bądzyńska²,

Sadowski³

2000

Exp. Physiol.

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Section: Discussionsupporting

confidence: 77%

Differential effect of frusemide on renal medullary and cortical blood flow in the anaesthetised rat

Dobrowolski¹,

Bądzyńska²,

Sadowski³

2000

Exp. Physiol.

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“…Cyclic AMP is the second messenger which mediates the effect of vasopressin on urea permeability (11) and osmotic water permeability (12) in rat terminal IMCDs. Hypertonic NaCl and vasopressin have additive effects on adenylyl cyclase activity and cyclic AMP production in the inner medulla (13)(14)(15)(16). To test whether hypertonic NaCl was increasing urea permeability by increasing cyclic AMP production, we pretreated tubules with 1 mM 8-bromo cyclic AMP.…”

Section: Experimental Animalsmentioning

confidence: 99%

An independent effect of osmolality on urea transport in rat terminal inner medullary collecting ducts.

Sands¹,

Schrader²

1991

J. Clin. Invest.

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We have shown that urea transport across the terminal inner medullary collecting duct (terminal IMCD) is mediated by a vasopressin-stimulated, facilitated diffusion process exhibiting properties consistent with a transporter. To investigate whether hypertonic NaCI, as exists in vivo in the inner medulla, affects urea permeability, we studied isolated perfused rat terminal IMCD segments. Perfusate and bath osmolality were varied symmetrically by adding or removing NaCI or mannitol. Urea permeability rose progressively when osmolality was increased with NaCI or mannitol from 290 to 690 mOsm/kg H20 in the absence of vasopressin; there was no further increase at 890 mOsm/kg H20. In the presence of 10-8 M arginine vasopressin, urea permeability increased when NaCI was added to raise osmolality from 290 to 490 mOsm/kg H20 but there was no further increase at 690 mOsm/kg H20. When 1 mM 8-bromo cyclic AMP was added to the bath, raising NaCI still increased urea permeability. These results suggest that urea transport across the rat terminal IMCD is regulated both by vasopressin and by osmolality at values present in the renal inner medulla. Osmolality seems to activate urea transport across the rat terminal IMCD by mechanisms distinct from those of vasopressin or cyclic AMP. (J. Clin. Invest. 1991.

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“…Since PGE is easily leaked out of cells and PGE2 receptors are located on the plasma membrane of renal epithelial cells, PGE derived from renal interstitial cells may antagonize the effect of AVP on the collecting tubule. Though we did not measure immunoreactive PGE2 without the addition of exogenous arachidonic acid, the osmotic effect may also affect the synthesis of arachidonic acid from phospholipid, as suggested by Craven and her associates (3).…”

Section: Discussionmentioning

confidence: 91%

“…The hypertonic medium but not the isotonic medium enhanced conversion of exogenous arachidonic acid to PGE2 and PGF2,,. Craven and DeRubertis have recently demonstrated that the hypertonic NaCl or mannitol stimulates immunoreactive PGE production by slices of the renal inner medulla, whereas urea inhibits this process (3). However, urea did not alter the conversion of exogenous arachidonic acid to PGE, indicating urea suppresses PGE synthesis through an inhibition of acyl hydrolase before a step of synthesis of arachidonic acid (4).…”

Section: Discussionmentioning

confidence: 99%

<b>EFFECTS OF OSMOLALITY ON THE INTERACTION BETWEEN VASOPRESSIN AND PROSTAGLANDINS IN RAT RENAL PAPILLARY COLLECTING TUBULE CELLS IN </b><b>CULTURE </b>

Ishikawa¹,

Saito²,

Kuzuya³

1985

Biomed. Res.

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The effect of environmental tonicity on the interaction between arginine vasopressin (AVP) and prostaglandin (PG) E in the production of adenosine-3',5'-cyclic monophosphate (cAMP) was studied in cultured rat renal papillary collecting tubule cells. Both AVP and PGE2 stimulated intracellular cAMP accumulation in a dose-dependent manner in an isotonic medium of 300 mOsm/kg H20 in the presence of a phosphodiesterase inhibitor. Environmental tonicity modulated the effect of AVP, PGE, or of both, on cellular cAMP production. The hypertonic medium with 1,800 mOsm/kg H20 enhanced the effect of 1><10_9 M AVP on the cellular cAMP production 1.6-fold, but markedly reduced the effect of 2 >< 10's M PGE, or PGE2 to one-sixth or one-third ofthat in the isotonic medium. The inhibitory effect of PGE, and PGE,»; on the action of AVP was not shown in the isotonic medium, but was seen in the hypertonic medium of 1,800 mOsm/kg H20. Synthesis of [3H]PGE2 was enhanced by hypertonicity, but not by AVP, when [3H]arachidonic acid was incorporated in the medium. The results indicate that an environmental tonicity is an important factor in modulating the effect ofPGE, or PGE2 on AVP-mediated cAMP production in renal papillary collecting tubule cells.

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Calcium-dependent action of osmolality on adenosine 3',5'-monophosphate accumulation in rat renal inner medulla: evidence for a relationship to calcium-responsive arachidonate release and prostaglandin synthesis.

Abstract: A B S T R A C T When urea and NaCl are employed as the major solutes of high osmolality buffers, the cyclic AMP (cAMP) content

Cited by 57 publications

References 43 publications

Differential effect of frusemide on renal medullary and cortical blood flow in the anaesthetised rat

Differential effect of frusemide on renal medullary and cortical blood flow in the anaesthetised rat

An independent effect of osmolality on urea transport in rat terminal inner medullary collecting ducts.

<b>EFFECTS OF OSMOLALITY ON THE INTERACTION BETWEEN VASOPRESSIN AND PROSTAGLANDINS IN RAT RENAL PAPILLARY COLLECTING TUBULE CELLS IN </b><b>CULTURE </b>

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