Objectives: This clinical study aimed to compare the clinical and radiographic success of CEM and two other pulpotomy materials in total pulpotomy applied to permanent teeth. Materials and Method: In our study, 48 permanent molar teeth with symptomatic irreversible pulpitis of 40 pediatric patients aged 5-12 years were included. Teeth were randomly divided into 3 groups. Total pulpotomy was performed with Calcium Hydroxide (CH), Mineral Trioxide Aggregate (MTA), and Calcium Enriched Material (CEM). Patients were invited for control at 3, 6, 12, 15, and 18 months for clinical and radiographic examination. The clinical and radiographic successes of 3 different materials were compared at all follow-up periods. Results: At the 6th, 12th, and 15th months, the MTA and CEM groups were statistically more successful than the CH group clinically and radiographically (p<0.05). The clinical and radiographic success rates of the CH, MTA, and CEM groups at the 18th month were 60%, 64.7%, and 68.8%, respectively, and there was no statistically significant difference between the 3 groups (p>0.05). Conclusions: We found that CEM was as successful as CH and MTA in the treatment of total pulpotomy applied to permanent molars with symptomatic irreversible pulpitis. Clinical Relevance: CEM is a promising material for different treatment modalities in pediatric dentistry and endodontics, without the major disadvantages of MTA.