2008
DOI: 10.1634/stemcells.2007-0591
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Calcium Handling in Human Embryonic Stem Cell-Derived Cardiomyocytes

Abstract: The objective of the current study was to characterize calcium handling in developing human embryonic stem cellderived cardiomyocytes (hESC-CMs). To this end, real-time polymerase chain reaction (PCR), immunocytochemistry, whole-cell voltage-clamp, and simultaneous patch-clamp/laser scanning confocal calcium imaging and surface membrane labeling with di-8-aminonaphthylethenylpridinium were used. Immunostaining studies in the hESC-CMs demonstrated the presence of the sarcoplasmic reticulum (SR) calcium release … Show more

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Cited by 158 publications
(176 citation statements)
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“…Therefore, interpretation of data regarding cardiomyogenesis efficiency and subtype identity must not only consider the presence and quantity of reference marker levels, but must consider the developmental stage(s) to which the timepoints of differentiation that are analyzed correspond. This is especially important considering that the maturation stage of cardiomyogenic cells generated by in vitro differentiation of hPSCs resembles most closely those of embryonic/fetal development [21][22][23][24][25] . Thus, relying on a marker's spatial expression in the postnatal heart may not be appropriate for the assessment of hPSCderived cells, at least in some cases.…”
Section: Introductionmentioning
confidence: 99%
“…Therefore, interpretation of data regarding cardiomyogenesis efficiency and subtype identity must not only consider the presence and quantity of reference marker levels, but must consider the developmental stage(s) to which the timepoints of differentiation that are analyzed correspond. This is especially important considering that the maturation stage of cardiomyogenic cells generated by in vitro differentiation of hPSCs resembles most closely those of embryonic/fetal development [21][22][23][24][25] . Thus, relying on a marker's spatial expression in the postnatal heart may not be appropriate for the assessment of hPSCderived cells, at least in some cases.…”
Section: Introductionmentioning
confidence: 99%
“…Consistent with its role in E-C coupling in the adult heart, Ca V 1.2 mRNA was detected as early as eight-week gestation in humans, with its level culminating to its maximum at adult stage (Qu and Boutjdir, 2001). Ca V 1.2 mRNA was also present in vitro at <40 days postbeating in H9.2 human ESC-(hESC-) derived CMs (Satin et al, 2008). In H7 hESC-derived CMs, Ca 2+ transients that were detected at day 17 post-differentiation could be eliminated by the application of 10μM diltiazem, an L-type specific antagonist; this signified the functionality of L-type channels by this time-point (Zhu et al, 2009).…”
Section: L-type Calcium Channelsmentioning
confidence: 71%
“…The electrophysiological properties were shown to be similar to mature differentiated cells in regard to ion channels and ionic currents [3,4]. The hESC-derived cardiomyocytes expressed a large sodium current density and the action potential was shortened by L-type Ca channel blockade.…”
Section: Stem Cells and Developmentmentioning
confidence: 88%
“…Many studies have been directed at Ca handling and ion channels in hESC-derived cardiomyocytes [1,3,18,19]. Ca involvement at stages of cell specification and commitment stages of the cardiac progenitor cells in vivo has received less attention.…”
Section: Discussionmentioning
confidence: 99%