Calcium Increases Xylella fastidiosa Surface Attachment, Biofilm Formation, and Twitching Motility

Cruz, Luisa F.; Cobine, Paul A.; Fuente, Leonardo De La

doi:10.1128/aem.06501-11

Cited by 143 publications

(152 citation statements)

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“…Previous research has shown that increasing amounts of calcium disable Bap-mediated biofilm formation and intercellular adhesion of Staphylococcus aureus (1). However, recent reports indicate that calcium promotes biofilm formation in other microorganisms, such as Xyllela fastidiosa or Vibrio vulnificus (4,7). Therefore, calcium may modulate biofilm formation in opposite ways in different bacteria, but the molecular basis for its role remains unclear.…”

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confidence: 87%

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Calcium Causes Multimerization of the Large Adhesin LapF and Modulates Biofilm Formation by Pseudomonas putida

et al. 2012

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LapF is a large secreted protein involved in microcolony formation and biofilm maturation in Pseudomonas putida. Its C-terminal domain shows the characteristics of proteins secreted through a type I secretion system and includes a predicted calcium binding motif. We provide experimental evidence of specific binding of Ca 2؉ to the purified C-terminal domain of LapF (CLapF). Calcium promotes the formation of large aggregates, which disappear in the presence of the calcium chelator EGTA. Immunolocalization of LapF also shows the tendency of this protein to accumulate in vivo in certain extracellular regions. These findings, along with results showing that calcium influences biofilm formation, lead us to propose a model in which P. putida cells interact with each other via LapF in a calcium-dependent manner during the development of biofilms.

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confidence: 87%

“…Calcium has been related to a variety of biological processes in bacteria (4,7,10), but its role in biofilm development is somewhat controversial. Previous research has shown that increasing amounts of calcium disable Bap-mediated biofilm formation and intercellular adhesion of Staphylococcus aureus (1).…”

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confidence: 99%

Calcium Causes Multimerization of the Large Adhesin LapF and Modulates Biofilm Formation by Pseudomonas putida

et al. 2012

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“…Growth rates were calculated as the slope of line obtained by natural logtransformed growth values at the exponential growth phase (approximately 1 to 4 days) using the formula {rate = [ln (OD day 4) -ln (OD day1)]/time}. Biofilm was quantified using the crystal violet assay, as previously described (Cruz et al 2012), at the end of the growth curve experiment (day 8). Settling rate, as a measure of cell-to-cell attachment, was determined by measuring OD 600 of a cell suspension in a cuvette when cells appeared to settle exponentially (5 to 30 min postinoculation [mpi]).…”

Section: Methodsmentioning

confidence: 99%

Natural Competence Rates Are Variable Among Xylella fastidiosa Strains and Homologous Recombination Occurs In Vitro Between Subspecies fastidiosa and multiplex

Kandel

Almeida

Cobine

et al. 2017

MPMI

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TitleNatural competence rates are variable among xylella fastidiosa strains and homologous recombination occurs in vitro between subspecies fastidiosa and multiplex Xylella fastidiosa, an etiological agent of emerging crop diseases around the world, is naturally competent for the uptake of DNA from the environment that is incorporated into its genome by homologous recombination. Homologous recombination between subspecies of X. fastidiosa was inferred by in silico studies and was hypothesized to cause disease emergence. However, no experimental data are available on the degree to which X. fastidiosa strains are capable of competence and whether recombination can be experimentally demonstrated between subspecies. Here, using X. fastidiosa strains from different subspecies, natural competence in 11 of 13 strains was confirmed with plasmids containing antibiotic markers flanked by homologous regions and, in three of five strains, with dead bacterial cells used as source of donor DNA. Recombination frequency differed among strains and was correlated to growth rate and twitching motility. Moreover, intersubspecific recombination occurred readily between strains of subsp. fastidiosa and multiplex, as demonstrated by movement of antibiotic resistance and green fluorescent protein from donor to recipient cells and confirmed by DNA sequencing of the flanking arms of recombinant strains. Results demonstrate that natural competence is widespread among X. fastidiosa strains and could have an impact in pathogen adaptation and disease development.

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“…Adding metals to the medium (Ca, Co, Cu, Fe and so on) [53], exposing bacteria to tissue cells it interacts with and feels tropism towards [54] as our work, are some methods employed in order to study and promote cell adhesion and twitching displacement. That is it, Phdp strain PP3 showed a slightly better displacement in culture media with a lot of cells; strains 94/99, DI21 and ATCC 17911 too, but a lower amount of added cells was enough and better; however, for the strain C2, media with or without cell debris did not make difference.…”

Section: Discussionmentioning

confidence: 99%

Effect of Different Culture Conditions on the (Twitching) Displacement of Photobacterium Damselae subsp. Piscicida

Vega¹,

Jose²,

Bravo³

et al. 2017

JBMOA

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Photobacterium damselae subsp. piscicida (Phdp), etiological agent of photobacteriosis, is a non-motile bacterium. Notwithstanding, it is able to move on solid surfaces. The presence of pili-like structures on the bacterial surface has been recently described and being associated with twitching displacement by our team. The objective of this study was to modify some conditions (temperature, pH, concentration of nutrients, salinity, solidification of the medium, surface roughness and presence of target cells of Phdp) to determine which ones promote motility on solid surfaces.Lower temperatures (≤ 18˚C), alkaline and acidic environments and lacking salinity (<0.5%) media had a negative effect on cell motility, while nutrient limitation did not affect the bacterial response. The best twitching motility was observed when Phdp was inoculated in medium with 0.2% agar and pH 7.0. Also, interesting results were obtained on rough surfaces or by the addition of cellular debris of SAF-1 cell line to the medium.According to the results, the "new" composition of the "twitching displacement" medium could be as follows: 3.7% (w/v) brain-heart infusion broth (BHIB) supplemented with about 1.0% (w/v) NaCl and 0.2% (w/v) agar, with a final pH of 7.0, and maybe, scrape petri dishes and add debris of SAF-1 cells 1:2-factor dilution to medium. If bacterial strains are incubated at 22-25˚C for three days, a good response should be achieved in terms of fimbriae production.

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Calcium Increases Xylella fastidiosa Surface Attachment, Biofilm Formation, and Twitching Motility

Cited by 143 publications

References 53 publications

Calcium Causes Multimerization of the Large Adhesin LapF and Modulates Biofilm Formation by Pseudomonas putida

Calcium Causes Multimerization of the Large Adhesin LapF and Modulates Biofilm Formation by Pseudomonas putida

Natural Competence Rates Are Variable Among Xylella fastidiosa Strains and Homologous Recombination Occurs In Vitro Between Subspecies fastidiosa and multiplex

Effect of Different Culture Conditions on the (Twitching) Displacement of Photobacterium Damselae subsp. Piscicida

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