Calcium-induced calcium release and gap junctions mediate large-scale calcium waves in olfactory ensheathing cells in situ

Olfactory ensheathing cells from the nasal mucosa and olfactory bulb have distinct membrane properties

“…Studies using OB slice preparations have revealed glutamatergic and purinergic receptor-mediated signaling between OSN axons and OECs (Rieger et al, 2007). This metabotropic mechanism involves a contribution from intracellular Ca 2+ stores (Hayat et al, 2003), Ca 2+ influx channels (Davies, Hayat, Wigley, & Robbins, 2004), and intracellular Ca 2+ waves mediated by gap junctions (Stavermann et al, 2015). There is evidence of several connexins expressed by the non-neuronal cells in the OB (Rash et al, 2005), and genetic deletion of Cx43 leads to the loss of intercellular gap junctional coupling between OECs (Piantanida et al, 2019).…”

Panglial gap junctions between astrocytes and olfactory ensheathing cells mediate transmission of Ca2+ transients and neurovascular coupling

“…In the olfactory bulb, astrocytes that contribute to a single glomerulus are coupled via gap‐junctions, forming a distinct functional unit (Roux, Benchenane, Rothstein, Bonvento, & Giaume, ). Moreover, OECs in the ONL show high connectivity and propagation of gap junction‐dependent Ca 2+ waves (Rela, Bordey, & Greer, ; Stavermann et al, ). Hence, both populations of olfactory bulb glial cells are gap junction‐coupled within each population, and we asked whether gap‐junctional coupling does also occur between those two different populations of glial cells.…”

“…In the olfactory bulb, astrocytes that contribute to a single glomerulus are coupled via gap-junctions, forming a distinct functional unit (Roux, Benchenane, Rothstein, Bonvento, & Giaume, 2011). Moreover, OECs in the ONL show high connectivity and propagation of gap junction-dependent Ca 2+ waves (Rela, Bordey, & Greer, 2010;Stavermann et al, 2015).…”

“…In addition to astrocytes, olfactory ensheathing cells (OECs), a specialized population of glial cells in the olfactory bulb (OB), are coupled by gap junctions and propagate intercellular Ca 2+ waves (Stavermann et al, 2015). Although cell bodies of astrocytes and OECs are spatially separated and organized in distinct networks, both respond to axon stimulation of olfactory sensory neurons (OSN) and the subsequent neurotransmitter release (glutamate and ATP) with Ca 2+ transients (Lohr, Grosche, Reichenbach, & Hirnet, 2014).…”

Photo-cleavable analog of BAPTA for the fast and efficient release of Ca2+

“…1 The fluctuations of intracellular Ca 2+ concentration regulate many cellular functions, such as neurotransmitter release, 2 operation of ion channels in the plasma membrane, 3 hormone secretion, 4 muscle contraction, 3 and many others. In recent years, the flash photolysis of photoresponsive calcium-ion chelators has become a common method in biochemical studies.…”