CALCIUM ION REQUIREMENT FOR PROLIFERATION OF BACTERIOPHAGE Φμ-4

Shafia, Fred; Thompson, Thomas L.

doi:10.1128/jb.88.2.293-296.1964

Cited by 27 publications

(11 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Omission of CaCl2 from T broth reduced the phage titer produced from 5.5 X 1010 to 7.3 X 106 PFU/ml. The enhancement of TP-84 phage production by Ca++ is similar to that reported for other thermophilic phages (18,19,23,24,26). The Ca++ effect has not been investigated further, but presumably Ca++ is required either for phage adsorption to host cells or for phage stability (1) or for both.…”

Section: Resultssupporting

confidence: 76%

Characterization of a Thermophilic Bacteriophage for Bacillus stearothermophilus

Saunders

Campbell

1966

J Bacteriol

View full text Add to dashboard Cite

, AND L. LEON CAMPBELL. Characterization of a thermophilic bacteriophage for Bacillus stearothermophilus. J. Bacteriol. 91:340-348. 1965.-The biological and physical-chemical properties of the thermophilic bacteriophage TP-84 were investigated. TP-84 was shown to be lytic for 3 of 24 strains of Bacillus stearothermophilus tested over the temperature range of 43 to 76 C. The latent period of TP-84 on B. stearothermophilus strain 10 was 22 to 24 min. TP-84 has a hexagonal head, 53 m,u in diameter and 30 m,u on a side; its tail is 130 m,u long and 3 to 5 m,u wide. The phage has an S'20 of 436, and bands at a density of 1.508 g/cc in CsCl (pH 8.5). The diffusion coefficient of TP-84

show abstract

Section: Resultssupporting

confidence: 76%

Characterization of a Thermophilic Bacteriophage for Bacillus stearothermophilus

Saunders

Campbell

1966

J Bacteriol

View full text Add to dashboard Cite

show abstract

“…The ejection of DNA from the head of a bacteriophage was induced by the increment of the ambient concentration of phosphate (Pi), a chelate agent [7][8][9]. When the concentration of Pi and Ca 2+ were higher than 10 mM and lower than 0.1 mM, respectively, the virions instantly ejected their DNA (Table 1 Fig.…”

Section: Ejection Of Dnamentioning

confidence: 99%

“…As the globular DNA cannot be packaged into capsids, packaging of DNA should be initiated when they are in a coiled state under the intracellular high Pi concentration. On the other hand, packaged DNA flows out of a capsid in the high Pi condition [7][8][9]. Indeed, if the packaging process is interrupted by the addition of ATPγS during the transportation process, the packaged DNA runs out from the capsid [37].…”

Section: Packaging Of Dnamentioning

confidence: 99%

“…The energy is stored as highly pressurized DNA, which is used as the force for ejection of the DNA into the host [2,6]. Treatment with chelate agents, like EDTA, induces the ejection of DNA from the head of the bacteriophage [7,8], and phages so treated did not recover their activity [7][8][9]. Among the ions which create ion potential on the cell membranes essential for the activity for life [10], calcium ion (Ca 2+ ) and inorganic orthophosphate ion (Pi) form the steepest gradients [11].…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

An ATP-free packaging of T4 DNA

Hara

Koike

2020

Preprint

View full text Add to dashboard Cite

Packaging of viral DNA into a capsid with the liquid crystalline density is a crucial step of the viral reproduction. The DNA packaging with the ATP fueled molecular motor is an established packaging system of viral DNA. However, the velocity and the efficiency are not well match with the in vivo processes. On the other hand, DNA compacts with the conformational change by multivalent cations exclusively of the valences higher than three. The conformational change of DNA was not considered as the vehicle of DNA packaging of viruses. Here, T4 DNA, ejected from a capsid in the ambient concentration of phosphate corresponding to the intra-cell concentration, is packaged into the capsid when the ambient concentration of phosphate decreases to the extra-cell concentration in the coexistence with divalent cations, Ca2+ and Mg2+. The compaction and packaging process coincide with the conformational change of DNA. Divalent cations can compact T4 DNA when the counter anion is phosphate. The DNA-packaged and re-generated virions showed equivalent infective ability with the original populations. The conformational changes of DNA between compact forms and coil forms were differentiated by the fluorescent microscopy. Identification between packaged- or unpackaged-DNA was confirmed by enzymatically. The infectious ability of the re-generated virions was confirmed by pfu. The concentration of ATP in the packaging process was measured by luminometric method. The packaging is proceeded in pM or lower concentration of ATP. This is a new packaging process of viral DNA practically free from ATP into which conformational change of DNA is incorporated. The conventional and new packaging processes may cooperate to accomplish the DNA packaging process of T4. The results may provide new interpretations of the life cycle of T4.

show abstract

“…Inactivation og phage by chelating agents and protection offered by divalent cations are not uncommon phenomena [10][11][12][13][14][15][16]. Inactivation of phage by chelating agents is usually interpreted to be due to complexing of cations present in the phage with the chelating agents.…”

Section: Inactivation Of Phage Particles As a Possible Mode Of Actionmentioning

confidence: 99%

Bacteriophage MB78 DNA synthesis is specifically inhibited by the chelating agent ethylene diamine tetraacetic acid

Verma

Chakravorty

1987

FEMS Microbiology Letters

View full text Add to dashboard Cite

The growth of bacteriophage MB78, a virulent phage of Salmonella typhimurium is extremely sensitive to the chelating agent EDTA. Other chelating agents like EGTA, a specific chelator for Ca2+ and orthophenanthroline which chelates Zn2+ and Fe2+ have no effect. EDTA stops phage MB78 DNA synthesis while synthesis of host DNA and other Salmonella phage DNA are not affected in presence of such low concentrations of EDTA. The present report indicates that some early phage function(s) and most probably the phage DNA synthesis are sensitive to EDTA which is probably due to chelation of Mg2+.

show abstract

CALCIUM ION REQUIREMENT FOR PROLIFERATION OF BACTERIOPHAGE Φμ-4

Abstract: SHAFIA, FRED (University of Nebraska, Lincoln), AND T. L. THOMPSON. Calcium ion requirement for proliferation of bacteriophage Ou-4. J. Bacteriol. 88:293-296. 1964.-Divalent ions are essential for proliferation of phage 4/u-4. Small

Cited by 27 publications

References 14 publications

Characterization of a Thermophilic Bacteriophage for Bacillus stearothermophilus

Characterization of a Thermophilic Bacteriophage for Bacillus stearothermophilus

An ATP-free packaging of T4 DNA

Bacteriophage MB78 DNA synthesis is specifically inhibited by the chelating agent ethylene diamine tetraacetic acid

Contact Info

Product

Resources

About