Calcium, iron, zinc and copper transport and uptake by Caco-2 cells in school meals: Influence of protein and mineral interactions

Cámara, F.; Barberá, Reyes; Amaro, Manuel; Farré, R.

doi:10.1016/j.foodchem.2005.11.010

Cited by 52 publications

(31 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This finding reinforces the important role of copper on the iron metabolism such as those previously reported by others (5,36), and could explain why high dialyzable copper fractions and copper dialyzability favor the iron dialysis in this in vitro study. On the other hand, others (35) did not find any significant interaction of the copper AUC on the iron AUC in healthy volunteers submitted to a supplementation trial (for a ratio of 1 mg copper/9 mg iron).…”

Section: Resultssupporting

confidence: 93%

Iron Dialyzability from Hospital Duplicate Meals: Daily Intake

Velasco-Reynold

Navarro-Alarcón

Serrana

et al. 2009

Biol Trace Elem Res

View full text Add to dashboard Cite

Both total and dialyzable iron levels and corresponding dialyzability were determined in 108 duplicate meals during 36 consecutive days. Total mean iron fraction of 5.90 +/- 4.97 mg was found in the meals. The iron supplied by the meals is directly and significantly (p < 0.05) correlated with macromicronutrient content (carbohydrates, fiber, and protein). The mean iron dialyzability (4.81 +/- 3.25%) was low and not significantly different among the three primary meals (breakfast, lunch, and dinner). Significant interactions of several minerals on iron levels were found (p < 0.05). Iron dialyzability was only statistically influenced by zinc dialyzability in meals (p < 0.05). The dialyzed iron fraction present in meals was significantly correlated with protein and ascorbic acid levels (p < 0.01). The mean iron daily dietary intake was 17.7 +/- 6.91 mg. The hospital meals provided enough iron. Foods of animal origin are primary sources of iron in diet.

show abstract

Section: Resultssupporting

confidence: 93%

Iron Dialyzability from Hospital Duplicate Meals: Daily Intake

Velasco-Reynold

Navarro-Alarcón

Serrana

et al. 2009

Biol Trace Elem Res

View full text Add to dashboard Cite

show abstract

“…4 HPLC chromatograms of organic acid concentrations in the apical chamber before (t 0 min) and after (t 120 min) the cell monolayer transport bioassay as well as the organic acid concentrations in the basolateral chamber at t 120 min; from banana (a) and sweet potato (b) Eur J Nutr (2011) 50: 31-40 37 studied in vitro. Many other nutrients such as carotenoids [26], iron [27,28], calcium [29,30] or polyphenols [27,31] have been studied for their intestinal bioavailability using a model of in vitro digestion alone or combining in vitro digestion/Caco-2 cell. However, in most of these studies, the digests were directly used for intestinal absorption without focusing on the nutrient release as a function of time per se.…”

Section: In Vitro Digestion and Release Of Organic Acidsmentioning

confidence: 99%

Organic acid bioavailability from banana and sweet potato using an in vitro digestion and Caco-2 cell model

et al. 2010

View full text Add to dashboard Cite

Introduction Organic acids from plant food have been shown to play an important role in the prevention of chronic diseases (osteoporosis, obesity), inherent to western diets, but little is known about their bioavailability in the small intestine, information that needs to be determined in order to quantify likely effects on human health. Methods An in vitro model of human digestion was carried out, comprising simulated oral, gastric and pancreatic digestion followed by an in vitro model of small intestine absorption using Caco-2 cell monolayers. As models for fruits and vegetables, freeze-dried or raw samples of banana and sweet potato were used. Results Organic acids have been found to be slowly released from the food matrix during simulated digestion of both banana and sweet potato, either raw or after freezedrying. In the Caco-2 cell assay, malic and oxalic acids were absorbed more than citric acid. Oxalic and citric acids, but not malic acid, were transported across the cell monolayer. The release and uptake of major organic acids from model fruits and vegetables using established in vitro simulation processes was not quantitative and varied with acid type.Conclusion Partial uptake is consistent with a dual nutritional role for organic acids as alkalinising agents (fraction which is taken up) and as modulators of large intestinal function (fraction which is not taken up in the small intestine). Studies of in vivo digestive release and uptake are needed in order to identify the contribution of organic acids to the nutritional value of fruits and vegetables.

show abstract

“…Oikeh et al (2003) reported on Fe/Zn bioavailability among maize genotypes using the Caco-2 cell model system and observed significant genotype · environment interactions on Fe/Zn bioavailability in maize grains. The Caco-2 cell system has been used to evaluate the effect of retinol on Fe bioavailability from Iranian bread (Gargari et al 2006)and to investigate the influence of protein and mineral interactions on the transport and uptake of Ca, Cu, Fe, and Zn in Spanish school children (Camara et al 2006). However, in China, little information has been obtained to date on the micronutrient bioavailability in edible parts of crops using the Caco-2 cell system.…”

Section: Technology For Evaluating Micronutrient Bioavailability In Fmentioning

confidence: 99%

“…Slight improvements in the concentration of these metabolic products would greatly increase the bioavailability of the micronutrients. Gargari et al (2006) in a study on an Iranian population found that retinol (vitamin A) enhanced Fe absorption in bread by 2.6-fold, while Camara et al (2006) in a study on Spanish children found that protein was able to significantly increase Fe absorption but not Zn absorption. A recent investigation by Miller et al using the pig model (2006, personal communication) demonstrated that inulin was able to enhance Fe bioavailability and absorption.…”

Section: Factors Controlling Micronutrient Bioavailability In Crops Fmentioning

confidence: 99%

Improving human micronutrient nutrition through biofortification in the soil–plant system: China as a case study

Yang

Chen

Feng

2007

Environ Geochem Health

197

108

View full text Add to dashboard Cite

Micronutrient malnutrition is a major health problem in China. According to a national nutritional survey, approximately 24% of all Chinese children suffer from a serious deficiency of iron (Fe) (anemia), while over 50% show a sub-clinical level of zinc (Zn) deficiency. More than 374 million people in China suffer from goiter disease, which is related to iodine (I) deficiency, and approximately 20% of the Chinese population are affected by selenium (Se) deficiency. Micronutrient malnutrition in humans is derived from deficiencies of these elements in soils and foods. In China, approximately 40% of the total land area is deficient in Fe and Zn. Keshan and Kaschin-Beck diseases always appear in regions where the soil content of Se in low. The soil-plant system is instrumental to human nutrition and forms the basis of the "food chain" in which there is micronutrient cycling, resulting in an ecologically sound and sustainable flow of micronutrients. Soil-plant system strategies that have been adopted to improve human micronutrient nutrition mainly include: (1) exploiting micronutrient-dense crop genotypes by studying the physiology and genetics of micronutrient flow from soils to the edible parts of crops; (2) improving micronutrient bioavailability through a better knowledge of the mechanisms of the enhancers' production and accumulation in edible parts and its regulation through soil-plant system; (3) improving our knowledge of the relationship between the content and bioavailability of micronutrients in soils and those in edible crop products for better human nutrition; (4) developing special micronutrient fertilizers and integrated nutrient management technologies for increasing both the density of the micronutrients in the edible parts of plants and their bioavailability to humans.

show abstract

Calcium, iron, zinc and copper transport and uptake by Caco-2 cells in school meals: Influence of protein and mineral interactions

Cited by 52 publications

References 29 publications

Iron Dialyzability from Hospital Duplicate Meals: Daily Intake

Iron Dialyzability from Hospital Duplicate Meals: Daily Intake

Organic acid bioavailability from banana and sweet potato using an in vitro digestion and Caco-2 cell model

Improving human micronutrient nutrition through biofortification in the soil–plant system: China as a case study

Contact Info

Product

Resources

About