Calcium oscillations in interstitial cells of the rabbit urethra

Johnston, Louise; Sergeant, Gerard P.; Hollywood, Mark A.; Thornbury, Keith D.; McHale, Noel G.

doi:10.1113/jphysiol.2004.078097

Cited by 70 publications

(111 citation statements)

References 35 publications

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“…Simultaneous recordings of intracellular calcium and electrical activity in ICCs revealed that intracellular calcium oscillations in ICCs were synchronized with slow waves, which indicates the close relationship between the intracellular calcium oscillations and the pacemaker activities of ICCs (Liu et al, 2005a;Liu et al, 2005b). Similar activities were also observed in ICCs of murine stomach and rabbit urethra (Johnston et al, 2005). Moreover, the evidence which 1,2-bis (2-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid acetoxymethyl ester (BAPTA-AM), an intracellular calcium chelator inhibited both pacemaker potentials and slow waves of murine intestine demonstrates the crucial role of intracellular calcium in the generation of pacemaker activities in ICCs (Kito et al, 2003).…”

Section: Intracellular Calcium Activitiessupporting

confidence: 50%

“…In the rabbit urethra ryanodine-operated calcium stores are considered to be prominent in the pacemaker activities in ICCs. The studies demonstrated that ryanodine abolished the intracellular calcium oscillations and pacemaker currents in ICCs from rabbit urethra, whereas 2-APB, an inhibitor of IP 3 receptor, partially but not completely inhibited the amplitude of the intracellular calcium oscillations (Johnston et al, 2005). Moreover, the calcium uptake by mitochondrion is also important for ICCs pacemaker activities in both gastrointestinal tract and urethra, because disruption of the mitochondiral membrane potential with the electron transport chain inhibitors rotenone and antimycin A and mitochondrial uncoupler carbonyl cyanide p-(trifluoromethoxy) phenylhydrazone (FCCP) and carbonyl cyanide m-chlorophenylhydrazone (CCCP) abolished both intracellular calcium oscillations and pacemaker currents in ICCs .…”

Section: Intracellular Calcium Activitiesmentioning

confidence: 99%

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Advances in Research on Pacemaking Function of Interstitial Cell of Cajal in Gastrointestinal Tract

Xu¹

2011

Modern Pacemakers - Present and Future

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Section: Intracellular Calcium Activitiessupporting

confidence: 50%

Section: Intracellular Calcium Activitiesmentioning

confidence: 99%

Advances in Research on Pacemaking Function of Interstitial Cell of Cajal in Gastrointestinal Tract

Xu¹

2011

Modern Pacemakers - Present and Future

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“…We have found that store depletion (CPA, thapsigargin), PLC inhibition (U73122) as well as blockade of IP 3 Rs abolished spontaneous Ca 2+ transients, indicating that the transients are due to release from cytoplasmic stores rather than Ca 2+ entry from extracellular space. Spontaneous cytosolic Ca 2+ elevations arising from the ER were previously described in many excitable and non-excitable isolated cell types, including neutrophils [31], myocytes [32,33], interstitial cells [34], astrocytes [35] and neurons [36]. However, in many of these cell preparations, spontaneous cytosolic calcium elevations occur at much higher frequencies, being rather oscillatory than stochastically distributed.…”

Section: Discussionmentioning

confidence: 99%

Spontaneous Ca 2+ transients in mouse microglia

et al. 2016

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Microglia are the resident immune cells in the central nervous system and many of their physiological functions are known to be linked to intracellular calcium (Ca 2+ ) signaling. Here we show that isolated and purified mouse microglia -either freshly or cultured -display spontaneous and transient Ca 2+ elevations lasting for around ten to twenty seconds and occurring at frequencies of around five to ten events per hour and cell. The events were absent after depletion of internal Ca 2+ stores, by phospholipase C (PLC) inhibition or blockade of inositol-1,4,5 trisphosphate receptors (IP 3 Rs), but not by removal of extracellular Ca 2+ , indicating that Ca 2+ is released from endoplasmic reticulum intracellular stores. We furthermore provide evidence that autocrine ATP release and subsequent activation of purinergic P2Y receptors is not the trigger for these events. Spontaneous Ca 2+ transients did also occur after stimulation with Lipopolysaccharide (LPS) and in glioma-associated microglia, but their kinetics differed from control conditions. We hypothesize that spontaneous Ca 2+ transients reflect aspects of cellular homeostasis that are linked to regular and patho-physiological functions of microglia.

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“…In a series of recent papers, ICC-like cells have been claimed to occur in other organs: vas deferens [25], urethra [26][27][28], bladder [29][30][31][32][33], ureter [34,35], uterus [36,37], blood vessels [38][39][40], lymphatics [41], prostate [42,43], penis [44], mammary gland [45] and pancreas [46].…”

Section: Introductionmentioning

confidence: 99%

Novel type of interstitial cell (Cajal-like) in human fallopian tube

et al. 2005

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We describe here ‐ presumably for the first time‐a Cajal‐like type of tubal interstitial cells (t‐ICC), resembling the archetypal enteric ICC. t‐ICC were demonstrated in situ and in vitro on fresh preparations (tissue cryosections and primary cell cultures) using methylene‐blue, crystal‐violet, Janus‐Green B or Mito Tracker‐Green FM Probe vital stainings. Also, t‐ICC were identified in fixed specimens by light microscopy (methylene‐blue, Giemsa, trichrome stainings, Gomori silver‐impregnation) or transmission electron microscopy (TEM). The positive diagnosis of t‐ICC was strengthened by immunohistochemistry (IHC; CD117/c‐kit+ and other 14 antigens) and immunofluorescence (IF; CD117/c‐kit+ and other 7 antigens). The spatial density of t‐ICC (ampullar‐segment cryosections) was 100–150 cells/mm2. Non‐conventional light microscopy (NCLM) of Epon semithin‐sections revealed a network‐like distribution of t‐ICC in lammina propria and smooth muscle meshwork. t‐ICC appeared located beneath of epithelium, in a 10–15μ thick ‘belt’, where 18±2% of cells were t‐ICC. In the whole lamina propria, t‐ICC were about 9%, and in muscularis ∼7%. In toto, t‐ICC represent ∼8% of subepithelial cells, as counted by NCLM. In vitro, t‐ICC were 9.9±0.9% of total cell population. TEM showed that the diagnostic ‘gold standard’ (Huizinga et al., 1997) is fulfilled by ‘our’ t‐ICC. However, we suggest a ‘platinum standard’, adding a new defining criterion ‐ characteristic cytoplasmic processes (number: 1–5; length: tens of μm; thickness: ±0.5μ; aspect: moniliform; braching: dichotomous; organization: network, labyrinthic‐system). Quantitatively, the ultrastructural architecture of t‐ICC is: nucleus, 23.6±3.2% of cell volume, with heterochromatin 49.1±3.8%; mitochondria, 4.8±1.7%; rough and smooth endoplasmic‐reticulum (1.1±0.6%, 1.0±0.2%, respectively); caveolae, 3.4±0.5%. We found more caveolae on the surface of cell processes versus cell body, as confirmed by IF for caveolins. Occasionally, the so‐called ‘Ca2+‐release units’ (subplasmalemmal close associations of caveolae+endoplasmic reticulum±mitochondria) were detected in the dilations of cell processes. Electrophysiological single unit recordings of t‐ICC in primary cultures indicated sustained spontaneous electrical activity (amplitude of field potentials: 57.26±6.56mV). Besides the CD117/c‐kit marker, t‐ICC expressed variously CD34, caveolins 1&2, α‐SMA, S‐100, vimentin, nestin, desmin, NK‐1. t‐ICC were negative for: CD68, CD1a, CD62P, NSE, GFAP, chromogranin‐A, PGP9.5, but IHC showed the possible existence of (neuro)endocrine cells in tubal interstitium. We call them ‘JF cells’. In conclusion, the identification of t‐ICC might open the door for understanding some tubal functions, e.g. pace‐making/peristaltism, secretion (auto‐, juxta‐ and/or paracrine), regulation of neurotransmission (nitrergic/purinergic) and intercellular signaling, via the very long processes. Furthermore, t‐ICC might even be uncommitted bipotential progenitor cells.

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Calcium oscillations in interstitial cells of the rabbit urethra

Abstract: Measurements were made (using fast confocal microscopy) of intracellular Ca 2+ levels in fluo-4 loaded interstitial cells isolated from the rabbit urethra.

Cited by 70 publications

References 35 publications

Advances in Research on Pacemaking Function of Interstitial Cell of Cajal in Gastrointestinal Tract

Advances in Research on Pacemaking Function of Interstitial Cell of Cajal in Gastrointestinal Tract

Spontaneous Ca 2+ transients in mouse microglia

Novel type of interstitial cell (Cajal-like) in human fallopian tube

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