Calcium Phosphate Nanocluster-Loaded Injectable Hydrogel for Bone Regeneration

Yao, Shasha; Xu, Yifei; Zhou, Yanyan; Shao, Changyu; Liu, Zhaoming; Jin, Biao; Zhao, Ruibo; Cao, Han; Pan, Haihua; Tang, Ruikang

doi:10.1021/acsabm.9b00270

Cited by 27 publications

(19 citation statements)

References 40 publications

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“…In addition, ACP is bioactive, with better biodegradability than crystalline CaP and with the ability to promote osteoblast adhesion [10] and osteconductivity [11]. These properties make ACP a promising candidate material for bone regeneration [9,[12][13][14], in applications such as bone cements [15] and bio-ceramics [16,17].…”

Section: Introductionmentioning

confidence: 99%

Highly Porous Amorphous Calcium Phosphate for Drug Delivery and Bio-Medical Applications

et al. 2019

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Amorphous calcium phosphate (ACP) has shown significant effects on the biomineralization and promising applications in bio-medicine. However, the limited stability and porosity of ACP material restrict its practical applications. A storage stable highly porous ACP with Brunauer–Emmett–Teller surface area of over 400 m2/g was synthesized by introducing phosphoric acid to a methanol suspension containing amorphous calcium carbonate nanoparticles. Electron microscopy revealed that the porous ACP was constructed with aggregated ACP nanoparticles with dimensions of several nanometers. Large angle X-ray scattering revealed a short-range atomic order of <20 Å in the ACP nanoparticles. The synthesized ACP demonstrated long-term stability and did not crystallize even after storage for over 14 months in air. The stability of the ACP in water and an α-MEM cell culture medium were also examined. The stability of ACP could be tuned by adjusting its chemical composition. The ACP synthesized in this work was cytocompatible and acted as drug carriers for the bisphosphonate drug alendronate (AL) in vitro. AL-loaded ACP released ~25% of the loaded AL in the first 22 days. These properties make ACP a promising candidate material for potential application in biomedical fields such as drug delivery and bone healing.

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Section: Introductionmentioning

confidence: 99%

Highly Porous Amorphous Calcium Phosphate for Drug Delivery and Bio-Medical Applications

et al. 2019

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“…Therefore, direct incorporation of calcium phosphate in various hydrogels is a common fabrication method for BTE applications. This approach is seen in diverse hydrogels, such as chitosan–gelatin, whey protein isolate–gelatin, PEG, poly(acrylic acid)–polyaspartic acid, and methacrylated gellan gum . Calcium phosphate can be self-assembled in a hydrogel network by incorporating calcium intriguing molecules, such as poly( l -glutamic acid) or 2D black phosphorus nanosheets .…”

Section: Biofunctionalization Of Hydrogelsmentioning

confidence: 99%

Rational Design of Hydrogels to Enhance Osteogenic Potential

Kim

Lee

2020

Chem. Mater.

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Bone tissue engineering (BTE) encompasses the field of biomaterials, cells, and bioactive molecules to successfully guide the growth and repair of bone tissue. Current BTE strategies rely on delivering osteogenic molecules or cells via scaffolding materials. However, growth factor- and stem cell-based treatments have several limitations, such as source restriction, low stability, difficulties in predicting long-term efficacy, and high costs, among others. These issues have promoted the development of material-based therapy with properties of accessibility, high stability, tunable efficacy, and low-cost production. Hydrogels are widely used in BTE applications because of their unique hydrophilic nature and tunable physicochemical properties to mimic the native bone environment. However, current hydrogel materials are not ideal candidates due to minimal osteogenic capability on their own. Therefore, recent studies of BTE hydrogels attempt to counterbalance these issues by modifying their biophysical properties. In this article, we review recent progress in the design of hydrogels to instruct osteogenic potential and present strategies developed to precisely control its bone healing properties.

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“…The physicochemical stability of the hydrogel scaffold is crucial during the bone tissue regeneration process, as the degradation rate might match bone ingrowth and promote subsequent remodeling and functional revive [40]. Figure 5 indicated the weight loss profile of the FG and FGSr scaffolds when immersed in SBF at 37 • C for a period of 4 weeks.…”

Section: Immersion Behaviormentioning

confidence: 99%

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

Wang

Liu

et al. 2020

Applied Sciences

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Cell-encapsulated bioscaffold is a promising and novel method to allow fabrication of live functional organs for tissue engineering and regenerative medicine. However, traditional fabrication methods of 3D scaffolds and cell-laden hydrogels still face many difficulties and challenges. This study uses a newer 3D fabrication technique and the concept of recycling of an unutilized resource to fabricate a novel scaffold for bone tissue engineering. In this study, fish-extracted gelatin was incorporated with bioactive ceramic for bone tissue engineering, and with this we successfully fabricated a novel fish gelatin methacrylate (FG) polymer hydrogel mixed with strontium-doped calcium silicate powder (FGSr) 3D scaffold via photo-crosslinking. Our results indicated that the tensile strength of FGSr was almost 2.5-fold higher as compared to FG thus making it a better candidate for future clinical applications. The in-vitro assays illustrated that the FGSr scaffolds showed good biocompatibility with human Wharton jelly-derived mesenchymal stem cells (WJMSC), as well as enhancing the osteogenesis differentiation of WJMSC. The WJMSC-laden FGSr 3D scaffolds expressed a higher degree of alkaline phosphatase activity than those on cell-laden FG 3D scaffolds and this result was further proven with the subsequent calcium deposition results. Therefore, these results showed that 3D-printed cell-laden FGSr scaffolds had enhanced mechanical property and osteogenic-related behavior that made for a more suitable candidate for future clinical applications. Furthermore, bone regeneration is a complex physiological process regulated by osteoclast resorption with osteoblast bone formation and the entire process includes inflammatory reactions, endochondral bone formation and bone remodeling. Currently, for clinical cases, the golden method to repair bone defects is to use autologous bone grafts as this method avoids disease spreading and immunological rejection. However, autografts have some major drawbacks such as limited availability and invasive harvesting that requires additional surgery to harvest healthy bones from another site. Furthermore, additional surgeries mean increased surgical risks and complications [2]. Allograft is an alternative to bone repair; however, it is highly limited due to rejection and lack of availability. To solve this problem, researchers had attempted to develop novel biomaterials including ceramics, polymers and composites as an alternative choice for bone regeneration [3][4][5]. In order to promote bone healing, the material must be biocompatible, biodegradable and able to promote bone regeneration which mimics the distinctive property of natural bone [6]. With the emergence of newer 3D printing techniques, we are now able to fabricate scaffolds with customized shape, pore sizes and architectures that are similar to native bone [7,8].For many years, bioceramics has emerged as promising biomaterial for bone regeneration [9,10]. Calcium phosphate is one of the most popular bioceramics which had been repeate...

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Calcium Phosphate Nanocluster-Loaded Injectable Hydrogel for Bone Regeneration

Cited by 27 publications

References 40 publications

Highly Porous Amorphous Calcium Phosphate for Drug Delivery and Bio-Medical Applications

Highly Porous Amorphous Calcium Phosphate for Drug Delivery and Bio-Medical Applications

Rational Design of Hydrogels to Enhance Osteogenic Potential

Enhanced Proliferation and Differentiation of Human Mesenchymal Stem Cell-laden Recycled Fish Gelatin/Strontium Substitution Calcium Silicate 3D Scaffolds

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