Calcium phosphate stability on melt electrowritten PCL scaffolds

Abbasi, Naghmeh; Hamlet, Stephen; Dau, Van Thanh; Nguyen, Nam‐Trung

doi:10.1016/j.jsamd.2020.01.001

Cited by 9 publications

(8 citation statements)

References 44 publications

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“…In this way, in view of the hydrophobic character of PCL and its poor cellular attachment ability, surface modification using plasma, alkali-treatment, or calcium phosphate (CaP) coatings has been investigated. [9] Remarkably, fluorapatite (FA), a bioceramic within the CaP family, has been shown to favorably influence osteogenic differentiation while also displaying antimicrobial action, which, in the case of periodontal regeneration, would be important for preventing bacterial colonization. [10] Moreover, Sikder et al reported on the bioactivity of fluoro-hydroxyapatite coating and its potential to form bone-like apatite globules.…”

Section: Introductionmentioning

confidence: 99%

A Highly Ordered, Nanostructured Fluorinated CaP‐Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration

Daghrery

Ferreira

Araújo

et al. 2021

Adv Healthcare Materials

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Periodontitis is a chronic inflammatory, bacteria-triggered disorder affecting nearly half of American adults. Although some level of tissue regeneration is realized, its low success in complex cases demands superior strategies to amplify regenerative capacity. Herein, highly ordered scaffolds are engineered via Melt ElectroWriting (MEW), and the effects of strand spacing, as well as the presence of a nanostructured fluorinated calcium phosphate (F/CaP) coating on the adhesion/proliferation, and osteogenic differentiation of human-derived periodontal ligament stem cells, are investigated. Upon initial cell-scaffold interaction screening aimed at defining the most suitable design, MEW poly(𝝐-caprolactone) scaffolds with 500 μm strand spacing are chosen. Following an alkali treatment, scaffolds are immersed in a pre-established solution to allow for coating formation. The presence of a nanostructured F/CaP coating leads to a marked upregulation of osteogenic genes and attenuated bacterial growth. In vivo findings confirm that the F/CaP-coated scaffolds are biocompatible and lead to periodontal regeneration when implanted in a rat mandibular periodontal fenestration defect model. In aggregate, it is considered that this work can contribute to the development of personalized scaffolds capable of enabling tissue-specific differentiation of progenitor cells, and thus guide simultaneous and coordinated regeneration of soft and hard periodontal tissues, while providing antimicrobial protection.

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Section: Introductionmentioning

confidence: 99%

A Highly Ordered, Nanostructured Fluorinated CaP‐Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration

Daghrery

Ferreira

Araújo

et al. 2021

Adv Healthcare Materials

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“…Recent literature has validated the use of argon, oxygen, air plasma, and combinations thereof to sterilize surfaces, increase hydrophilicity and functionalize surfaces with oxygen species and coatings to promote cell attachment. [ 10 ] However, despite the well‐studied benefits of plasma for improving the adhesion of cells onto PCL fibers, [ 11,12 ] exposure to plasma may also contribute to the accelerated oxidative degradation of scaffolds, limiting longevity and mechanical stability in vitro and in vivo. [ 13 ] This is of particular concern when considering MEW scaffolds, owing to their order‐of‐magnitude higher fiber surface‐to‐volume ratio, which significantly increases their susceptibility to degradation via surface modifications compared to fused deposition modelling (FDM)‐printed scaffolds with the same porosity (Figure S1 and Table S1, Supporting Information).…”

Section: Introductionmentioning

confidence: 99%

Degradation of Melt Electrowritten PCL Scaffolds Following Melt Processing and Plasma Surface Treatment

Paxton

Tuten

et al. 2021

Macromol. Rapid Commun.

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Melt electrowriting (MEW) has been widely used to process polycaprolactone (PCL) into highly ordered microfiber scaffolds with controllable architecture and geometry. However, the integrity of PCL during specific processes involved in routine MEW scaffold development has not yet been thoroughly investigated. This study investigates the impact of MEW processing on PCL following exposure to high temperatures required for melt extrusion as well as atmospheric plasma, a widely used surface treatment for improving MEW scaffold hydrophilicity. The change in polymer molecular weight and melt temperature is characterized, in comparing unprocessed and processed samples, in addition to analysis of the mechanical and surface properties of the scaffolds. No significant difference in the molecular weight or mechanical properties of the PCL scaffolds is evident following 5 days of cyclic heating to 90 °C. Exposure to plasma for up to 5 min significantly increased hydrophilicity and surface adhesion force, characterized via contact angle and atomic force microscope, however, significant polymer degradation occurred evidenced by increased brittleness of the scaffolds. This study demonstrates the degradation of PCL following fabrication via MEW and surface treatment to guide the optimization of scaffold development for subsequent applications in tissue engineering and biofabrication.

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“…In this study, the maximum melt-electrowritable concentration of chitosan loaded into PCL was 1%, with the flow and strand thickness consistency inversely proportional to the chitosan concentration. Previous studies by other groups have investigated surface modifications of PCL scaffolds to improve the cellular activity on melt elctrowritten scaffolds, ,,, with one investigation by our group having incorporated milk proteins within PCL. The upper limit of the milk proteins for MEW was 0.5% (lactoferrin and whey protein) in PCL .…”

Section: Discussionmentioning

confidence: 99%

“…To date, melt-electrowritten scaffolds have been investigated in the skin, heart valves, and musculoskeletal soft tissues, − where poly-ε-caprolactone (PCL) is commonly used. PCL is a thermoplastic, synthetic, aliphatic polyester that has been combined with other biomaterials, such as milk protein and calcium phosphate, or functionalized with bioactive groups, i.e., poly(hydroxymethylglycolide- co -ε-caprolactone), to increase PCL bioactivity. , PCL, a Food and Drug Administration-approved bioresorbable polymer, is the biomaterial of choice for designing tissue-engineered melt-electrowritten scaffolds due to its easy processability, low cost, and lack of immunogenicity . Its controllable mechanical properties, slow rate of degradation, chemical versatility, and high elasticity are advantageous to applications that require in vivo durability and longevity.…”

Section: Introductionmentioning

confidence: 99%

Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior

Yoshida

Turner

Ali

et al. 2021

ACS Appl. Bio Mater.

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Melt electrowriting (MEW) is an emerging technique that precisely fabricates microfibrous scaffolds, ideal for tissue engineering, where biomimetic microarchitectural detail is required. Polycaprolactone (PCL), a synthetic polymer, was selected as the scaffold material due to its biocompatibility, biodegradability, mechanical strength, and melt processability. To increase PCL bioactivity, a natural polymer, chitosan, was added to construct MEW fibrous composite scaffolds. To date, this is the first study of its kind detailing the effects of stem cell behavior on PCL containing chitosan MEW scaffolds. The aim of this study was to melt electrowrite a range of PCL/chitosan tissue-engineered constructs (TECs) and assess their suitability to promote the growth of human bone-marrow-derived mesenchymal stem cells (hBMSCs). In vitro physical and biological characterizations of melt-electrowritten TECs were performed. Physical characterization showed that reproducible, layered micron-range scaffolds could be successfully fabricated. As well, cell migration and proliferation were assessed via an assay to monitor cell infiltration throughout the three-dimensional (3D) melt-electrowritten scaffold structure. A statistically significant increase (∼140%) in hBMSC proliferation in 1 wt % chitosan PCL blends in comparison to PCL-only scaffolds was found when monitored over two weeks. Overall, our study demonstrates the fabrication of melt-electrowritten PCL/chitosan composite scaffolds with controlled microarchitecture and their potential use for regenerative, tissue engineering applications.

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Calcium phosphate stability on melt electrowritten PCL scaffolds

Cited by 9 publications

References 44 publications

A Highly Ordered, Nanostructured Fluorinated CaP‐Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration

A Highly Ordered, Nanostructured Fluorinated CaP‐Coated Melt Electrowritten Scaffold for Periodontal Tissue Regeneration

Degradation of Melt Electrowritten PCL Scaffolds Following Melt Processing and Plasma Surface Treatment

Three-Dimensional Melt-Electrowritten Polycaprolactone/Chitosan Scaffolds Enhance Mesenchymal Stem Cell Behavior

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