Calcium phosphates Chitosan-Xanthan composite scaffolds associated with mesenchymal stem cells for regenerative dentistry application

Neves, José Guilherme; Rocha, Daniel Navarro da; Lopes, Camila Cristine; Barbosa, Rafael Maza; Ferreira, Lorena Fernandes; Westin, Cecília Buzatto; Moraes, Ângela Maria; Calsa, Bruno; Santamaria‐Jr, Milton; Correr-Sobrinho, Lourenço; Correr, Américo Bortolazzo

doi:10.1016/j.ceramint.2022.04.288

Cited by 10 publications

(19 citation statements)

References 41 publications

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“…These results suggest that the HA powder incorporation into the C-X polyelectrolyte complex does not change the HA crystallinity and phase characteristics, despite the use of acetic acid for chitosan dissolution [ 29 ]. Neves et al [ 22 ] found results similar to those described in this work for porous scaffolds composed of C-X and HA. In that work, the researchers also observed the crystalline phase of HA in the scaffolds.…”

Section: Resultssupporting

confidence: 88%

“…These results suggest that with the increase in the vibration of the bands related to -PO 4 −3 , interactions within the polysaccharide matrix via hydrogen bonds or ionic interactions with Ca 2+ could have occurred [ 27 , 28 ]. In these spectra, the region between 3400 cm −1 and 3500 cm −1 corresponds to water sorption by HA and polysaccharides [ 22 , 39 ]. Close bands between 1030 and 1090 cm −1 were also reported for the production of scaffolds for bone healing composed of chitosan and hydroxyapatite [ 39 ].…”

Section: Resultsmentioning

confidence: 99%

“…These biomaterials are capable of absorbing high quantities of biological aqueous fluids and culture medium and are able to effectively sustain cells inoculated in them. Thus, chitosan-xanthan gum-based PECs may be considered attractive materials for application in GBR or GTR [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

“…Ideally, GBR membranes should not only act as barriers but also show bioactive properties to lead to a specific cellular response and accelerate bone regeneration in the bone defect site. Therefore, the incorporation of calcium phosphates in these biomaterials may consist of a relevant strategy to further improve their mechanical and biological properties for bone tissue regeneration application [ 22 , 23 ]. Hydroxyapatite is a bioactive ceramic compound and, due to its similarity to the bone mineral phase, it has been applied as a bone graft component or as coatings of inert metallic implants [ 24 , 25 , 26 ].…”

Section: Introductionmentioning

confidence: 99%

“…Hydroxyapatite is a bioactive ceramic compound and, due to its similarity to the bone mineral phase, it has been applied as a bone graft component or as coatings of inert metallic implants [ 24 , 25 , 26 ]. HA properties, such as biocompatibility and osteoconductivity, can increase cell activity, promoting local bone defect mineralization and repair [ 22 , 27 ]. Some efforts have already been made to produce biomaterials using natural polymers in combination with calcium phosphate.…”

Section: Introductionmentioning

confidence: 99%

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Cell-Friendly Chitosan-Xanthan Gum Membranes Incorporating Hydroxyapatite Designed for Periodontal Tissue Regeneration

Barbosa

Rocha²,

Souza

et al. 2023

Pharmaceutics

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In this work, a simple method was proposed to produce dense composite polysaccharide-based membranes to be used for guided tissue and guided bone regeneration. The mucoadhesive polysaccharides chitosan (C) and xanthan gum (X) were used to produce polyelectrolyte-based complex membranes. Hydroxyapatite (HA) was added to the formulation as a potential drug carrier, in C:X:HA mass proportions equal to 1:1:0.4, 1:1:2, and 1:1:10, and also to improve membranes bioactivity and biomimetic properties. FTIR analysis indicated successful incorporation of HA in the membranes and XRD analysis showed that no changes in the HA crystalline structure were observed after incorporation. The residual mass evaluated by TGA was higher for the formulation produced at the proportion 1:1:10. The membranes produced showed asymmetrical surfaces, with distinct roughness. Increasing the HA concentration increased the surface roughness. Greater in vitro proliferation of dental pulp mesenchymal stem cells was observed on the surface of the membrane with 1:1:10 C:X:HA proportion. However, the 1:1:2 formulation showed the most adequate balance of mechanical and biological properties. These results suggest that adding HA to the membranes can influence mechanical parameters as well as cell adhesion and proliferation, supporting the potential application of these materials in regenerative techniques and the treatment of periodontal lesions.

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Section: Resultssupporting

confidence: 88%