Calcium promotes activity and confers heat stability on plant peroxidases

Plieth, Christoph; Vollbehr, Sonja

doi:10.4161/psb.20065

Cited by 34 publications

(15 citation statements)

References 65 publications

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“…Although the level of Ca 2+ in olive apoplast has not been reported, its concentration is likely sufficient to guarantee Ca 2+ influx in the Psav cells. In fact, the Ca 2+ concentrations used in this study are consistent with those reported in plant apoplast (Hepler, ; Plieth and Vollbehr, ), which range from 10 µM to 10 mM. In addition, during the early phase of bean infection with avirulent and virulent Pseudomonas savastanoi pv.…”

Section: Discussionsupporting

confidence: 89%

A Na⁺/Ca²⁺ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence

Moretti

Trabalza

Granieri

et al. 2019

Molecular Plant Pathology

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Summary In a number of compatible plant‐bacterium interactions, a rise in apoplastic Ca 2+ levels is observed, suggesting that Ca 2+ represents an important environmental clue, as reported for bacteria infecting mammalians. We demonstrate that Ca 2+ entry in Pseudomonas savastanoi pv. savastanoi ( Psav ) strain DAPP‐PG 722 is mediated by a Na + /Ca 2+ exchanger critical for virulence. Using the fluorescent Ca 2+ probe Fura 2‐AM, we demonstrate that Ca 2+ enters Psav cells foremost when they experience low levels of energy, a situation mimicking the apoplastic fluid. In fact, Ca 2+ entry was suppressed in the presence of high concentrations of glucose, fructose, sucrose or adenosine triphosphate (ATP). Since Ca 2+ entry was inhibited by nifedipine and LiCl, we conclude that the channel for Ca 2+ entry is a Na + /Ca 2+ exchanger. In silico analysis of the Psav DAPP‐PG 722 genome revealed the presence of a single gene coding for a Na + /Ca 2+ exchanger ( cneA ), which is a widely conserved and ancestral gene within the P. syringae complex based on gene phylogeny. Mutation of cneA compromised not only Ca 2+ entry, but also compromised the Hypersensitive response (HR) in tobacco leaves and blocked the ability to induce knots in olive stems. The expression of both pathogenicity ( hrpL , hrpA and iaaM ) and virulence ( ptz ) genes was reduced in this Psav ‐ cneA mutant. Complementation of the Psav ‐ cneA mutation restored both Ca 2+ entry and pathogenicity in olive plants, but failed to restore the HR in tobacco leaves. In conclusion, Ca 2+ entry acts as a ‘host signal’ that allows and promotes Psav pathogenicity on olive plants.

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Section: Discussionsupporting

confidence: 89%

A Na⁺/Ca²⁺ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence

Moretti

Trabalza

Granieri

et al. 2019

Molecular Plant Pathology

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“…ions and in absence of Ca 2? , POD is functionally inactive (Plieth and Vollbehr 2012). On the other hand, salt stress might have limited the Ca 2?…”

Section: Discussionmentioning

confidence: 99%

Physiological mechanisms of exogenous calcium on alleviating salinity-induced stress in rice (Oryza sativa L.)

Roy

Tahjib‐Ul‐Arif

Polash

et al. 2019

Physiol Mol Biol Plants

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Being more sensitive to salt stress among the cereals, growth of rice (Oryza sativa L.) has been habitually affected by salinity. Although, several practices have evolved to sustain the growth of rice under salinity, the enormous role of calcium (Ca 2?) as a signalling molecule in salt stress mitigation is still arcane. Considering this fact, an experiment was performed aiming to explicate the mechanism of salt-induced growth inhibition in rice and its alleviation by exogenous Ca 2?. At germination stage, 10 mM and 15 mM CaCl 2 primed rice (cv. Binadhan-10 & Binadhan-7) seeds were grown in petri dishes for 9 days under 100 mM NaCl stress. At seedling stage, 9-day-old rice seedlings grown on sand were exposed to 100 mM NaCl alone and combined with 10 mM and 15 mM CaCl 2 for 15 days. This research revealed that salinity radically slowed down growth of rice seedlings and Ca 2? treatment noticeably improved growth performances. At germination stage, 10 mM CaCl 2 treatment significantly increased the final germination percentage, germination rate index (in Binadhan-7), shoot, root length (89.20, 67.58% in Bindhan-10 & 84.72, 31.15% in Bindhan-7) and biomass production under salinity. Similarly, at seedling stage, 10 mM CaCl 2 supplementation in salt-stressed plants enhanced shoot length (42.17, 28.76%) and shoot dry weight (339.52, 396.20%) significantly in Binadhan-10 & Binadhan-7, respectively, but enhanced root dry weight (36.76%) only in Binadhan-10. In addition, 10 mM CaCl 2 supplementation on salt-stressed seedlings increased the chlorophyll and proline content, and oppressed the accretion of reactive oxygen species thus protecting from oxidative damage more pronouncedly in Binadhan-10 than Binadhan-7 as reflected by the elevated levels of catalase and ascorbate peroxidase activity. The 15 mM CaCl 2 somehow also enhanced some growth parameters but overall was less effective than 10 mM CaCl 2 to alleviate salt stress, and sometimes showed negative effect. Therefore, supplementary application of calcium-rich fertilizers in saline prone soils can be an effective approach to acclimatize salt stress and cultivate rice successfully.

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“…Different hypotheses have been proposed to explain the molecular basis of annexin-mediated alleviation of oxidative stress, including innate peroxidase activity [ 21 , 139 , 140 ], calcium-induced stabilization of peroxidases activity [ 141 ], and modulation of calcium influx [ 142 , 143 ]. Based on the results of our experiments, we assume that annexin-mediated reduction in oxidative stress in transgenic potato overexpressing STANN1 results from the annexin effect on thiol-disulfide homeostasis.…”

Section: Discussionmentioning

confidence: 99%

Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

et al. 2015

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Annexins are a family of calcium- and membrane-binding proteins that are important for plant tolerance to adverse environmental conditions. Annexins function to counteract oxidative stress, maintain cell redox homeostasis, and enhance drought tolerance. In the present study, an endogenous annexin, STANN1, was overexpressed to determine whether crop yields could be improved in potato (Solanum tuberosum L.) during drought. Nine potential potato annexins were identified and their expression characterized in response to drought treatment. STANN1 mRNA was constitutively expressed at a high level and drought treatment strongly increased transcription levels. Therefore, STANN1 was selected for overexpression analysis. Under drought conditions, transgenic potato plants ectopically expressing STANN1 were more tolerant to water deficit in the root zone, preserved more water in green tissues, maintained chloroplast functions, and had higher accumulation of chlorophyll b and xanthophylls (especially zeaxanthin) than wild type (WT). Drought-induced reductions in the maximum efficiency and the electron transport rate of photosystem II (PSII), as well as the quantum yield of photosynthesis, were less pronounced in transgenic plants overexpressing STANN1 than in the WT. This conferred more efficient non-photochemical energy dissipation in the outer antennae of PSII and probably more efficient protection of reaction centers against photooxidative damage in transgenic plants under drought conditions. Consequently, these plants were able to maintain effective photosynthesis during drought, which resulted in greater productivity than WT plants despite water scarcity. Although the mechanisms underlying this stress protection are not yet clear, annexin-mediated photoprotection is probably linked to protection against light-induced oxidative stress.

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Calcium promotes activity and confers heat stability on plant peroxidases

Cited by 34 publications

References 65 publications

A Na⁺/Ca²⁺ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence

A Na⁺/Ca²⁺ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence

Physiological mechanisms of exogenous calcium on alleviating salinity-induced stress in rice (Oryza sativa L.)

Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

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Calcium promotes activity and confers heat stability on plant peroxidases

Cited by 34 publications

References 65 publications

A Na+/Ca2+ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence

A Na+/Ca2+ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence

Physiological mechanisms of exogenous calcium on alleviating salinity-induced stress in rice (Oryza sativa L.)

Potato Annexin STANN1 Promotes Drought Tolerance and Mitigates Light Stress in Transgenic Solanum tuberosum L. Plants

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A Na⁺/Ca²⁺ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence

A Na⁺/Ca²⁺ exchanger of the olive pathogen Pseudomonas savastanoi pv. savastanoi is critical for its virulence