Calcium pyrophosphate crystal deposition disease: Preparation and characterization of crystals

Mandel, Gretchen S.; Renne, Kathleen M.; Kolbach, Ann M.; Kaplan, Wayne D.; Miller, Jay D.; Mandel, Neil S.

doi:10.1016/0022-0248(88)90093-0

Cited by 29 publications

(47 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The X-ray powder diffraction patterns of both the m-CPPD and t-CPPD crystals gave d-spacings ( Table 1) that matched those previously reported [21,23]. m-CPPD crystals gave DSC scans that showed two endothermic peaks, each corresponding to the loss of 1 mol of water of hydration.…”

Section: Resultssupporting

confidence: 69%

See 1 more Smart Citation

The interaction of monoclinic calcium pyrophosphate dihydrate crystals with neutrophils

1996

View full text Add to dashboard Cite

Monoclinic calcium pyrophosphate dihydrate (m-CPPD) crystals were synthesized and characterized using physical methods, IgG binding to m-CPPD crystals was quantitated, and the effect of IgG or plasma opsonization on m-CPPD-induced neutrophil activation was determined. Adsorption of IgG to crystals was measured using fluorescent-labelled FITC-IgG. Neutrophil activation by uncoated m-CPPD and crystals precoated with IgG or plasma was measured using luminol-enhanced chemiluminescence, superoxide anion generation, and myeloperoxidase release. m-CPPD bound small (compared to triclinic CPPD) but significant amounts of IgG and induced a strong activation of neutrophils at low concentrations of crystals. The rate and extent of chemiluminescence, superoxide anion production, and degranulation was not affected by precoating m-CPPD crystals with IgG during the early phase of neutrophil responses, but was inhibited by the precoating of crystals with plasma.

show abstract

Section: Resultssupporting

confidence: 69%

“…These crystals were used to synthesize both m-CPPD and t-CPPD, t-CPPD crystals were prepared as previously reported by Burt and Jackson [22]. m-CPPD crystals were prepared based on the method reported by Mandel et al [23] with minor modifications. In a 250-ml Erlenmeyer flask, 106 ml of distilled water was heated at 50 °C.…”

Section: Synthesis Of Crystalsmentioning

confidence: 99%

The interaction of monoclinic calcium pyrophosphate dihydrate crystals with neutrophils

1996

View full text Add to dashboard Cite

show abstract

“…1. The 2Â and d-spacing obtained matched those previously reported (Brown et al, 1963;Mandel et al, 1988).…”

Section: X-ray Powder Diffraction (Crystals a And B)supporting

confidence: 89%

“…Since in vivo t-CPPD crystals have been observed to have the shape of a parallelogram of length 1-20 m (Rosenthal and Ryan, 2004), we synthesized some small single crystals using the method reported by Brown et al (1963) with slight modifications: A solution of 1.1 g of CaH 2 P 2 O 7 in 100 ml of deionized water was kept for 7 days with continuous stirring at room temperature. After verification of the presence of single crystals of about 10 m using polarized microscopy, the crystals were filtered out and isolated (Watanabe, 1991) We prepared t-CPPD according to the method of Mandel et al (1988) for sufficiently large crystals in order to perform the single crystal X-ray diffraction measurements (crystal B).…”

Section: Preparation Of T-cppdmentioning

confidence: 99%

“…We first prepared single crys-0968-4328/$ -see front matter © 2010 Elsevier Ltd. All rights reserved. doi:10.1016/j.micron.2010.07.001 tals of triclinic calcium pyrophosphate dihydrate (t-CPPD), because the crystal structure of this polymorph has been known from single crystal X-ray diffraction studies (Mandel, 1975), whereas more detailed studies than crystallographic lattice constants and parameters have not been reported on the crystal structure of the other polymorph (monoclinic, m-CPPD) (Brown et al, 1963;Mandel et al, 1988). Both polymorphs have been observed in vivo (Rosenthal and Ryan, 2004).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Problems in polarized light microscopy observation of birefringence of calcium pyrophosphate dihydrate crystals

Omura

Okamoto

Konno

et al. 2010

Micron

View full text Add to dashboard Cite

Serum and plasma inhibit neutrophil stimulation by hydroxyapatite crystals. evidence that serum αl2‐hs glycoprotein is a potent and specific crystal‐bound inhibitor

Terkeltaub

Santoro

Mandel

et al. 1988

Arthritis & Rheumatism

View full text Add to dashboard Cite

Tissue deposits of basic calcium phosphate (BCP) crystals are associated with various clinical manifestations of inflammation. We addressed the possibility that native proteins modify the ability of hydroxyapatite (HA) crystals to stimulate human inflammatory cells. Neutrophil superoxide release and chemiluminescence in response to HA crystals (0.3-4.0 mg/ml) were blunted by serum and plasma. Inhibitory activity was progressively removed from serum by sequential adsorption with HA crystals, suggesting that the inhibitors were crystal-bound proteins. Thus, we characterized HA crystal-bound plasma proteins by O'Farrell gels: Fibronectin, transferrin, albumin, a,-HS glycoprotein (AHSG), al-proteinase inhibitor, a,-acid glycoprotein, Gc globulin, haptoglobin, and high density lipoprotein apolipoproteins were major bound species. Of these, AHSG was the most active inhibitor of HA-induced neutrophil superoxide release, and this glycoprotein partially (60%) restored inhibitory activity to HAadsorbed serum. AHSG also bound in vitro to the related BCP crystal, octacalcium phosphate, but only minimally to calcium pyrophosphate dihydrate crystals and monosodium urate crystals. Suppressive effects on neutrophil stimulation exhibited by AHSG were also specific for BCP crystals. AHSG was present in noninflammatory synovial fluids bound to synthetic HA crystals in vitro, and AHSG could be detected on native synovial fluid HA crystals. We conclude that the binding of AHSG may modulate the inflammatory potential of BCP crystals.Synthetic hydroxyapatite (HA) crystals directly stimulate human neutrophils in vitro (1) and consistently provoke self-limited acute inflammation when injected into the articular and pleural cavities of animals, into the foot pad of rats, and into the dermis of human volunteers (2-4). Furthermore, acute periarticular inflammation, and less commonly, an acute synovitis that is similar to acute gout in presentation and in response to therapy, are associated with the presence of HA crystals and related basic calcium phosphates (BCP) (2,3,5-8).

show abstract

Calcium pyrophosphate crystal deposition disease: Preparation and characterization of crystals

Cited by 29 publications

References 10 publications

The interaction of monoclinic calcium pyrophosphate dihydrate crystals with neutrophils

The interaction of monoclinic calcium pyrophosphate dihydrate crystals with neutrophils

Problems in polarized light microscopy observation of birefringence of calcium pyrophosphate dihydrate crystals

Serum and plasma inhibit neutrophil stimulation by hydroxyapatite crystals. evidence that serum αl2‐hs glycoprotein is a potent and specific crystal‐bound inhibitor

Contact Info

Product

Resources

About