Calcium release from Synechocystis cells induced by depolarization of the plasma membrane: MscL as an outward Ca2+ channel

Nazarenko, Lyudmila V.; Andreev, I. M.; Lyukevich, Alexander A.; Pisareva, Tatiana; Los, Dmitry A.

doi:10.1099/mic.0.26074-0

Cited by 44 publications

(28 citation statements)

References 39 publications

(39 reference statements)

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“…The gene (slr0875) encoded a high-conductance mechanosensitive channel; such channels are present in bacterial membranes and open in response to stretch forces in the lipid bilayer, preventing cell lysis (23). In addition, activation of Slr0875 was shown to result in Ca 2ϩ release (31). Increased abundance of the transcript for this channel may be part of a response directed at maintaining ion homeostasis at high pH.…”

Section: Resultsmentioning

confidence: 99%

Global Transcriptional Response of the Alkali-Tolerant CyanobacteriumSynechocystissp. Strain PCC 6803 to a pH 10 Environment

Summerfield

Sherman

2008

Appl Environ Microbiol

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Many cyanobacterial strains are able to grow at a pH range from neutral to pH 10 or 11. Such alkaline conditions favor cyanobacterial growth (e.g., bloom formation), and cyanobacteria must have developed strategies to adjust to changes in CO 2 concentration and ion availability. Synechocystis sp. strain PCC 6803 exhibits similar photoautotrophic growth characteristics at pH 10 and pH 7.5, and we examined global gene expression following transfer from pH 7.5 to pH 10 to determine cellular adaptations at an elevated pH. The strategies used to develop homeostasis at alkaline pH had elements similar to those of many bacteria, as well as components unique to phototrophic microbes. Some of the response mechanisms previously identified in other bacteria included upregulation of Na ؉ /H ؉ antiporters, deaminases, and ATP synthase. In addition, upregulated genes encoded transporters with the potential to contribute to osmotic, pH, and ion homeostasis (e.g., a water channel protein, a large-conductance mechanosensitive channel, a putative anion efflux transporter, a hexose/proton symporter, and ABC transporters of unidentified substrates). Transcriptional changes specific to photosynthetic microbes involved NADH dehydrogenases and CO 2 fixation. The pH transition altered the CO 2 /HCO 3 ؊ ratio within the cell, and the upregulation of three inducible bicarbonate transporters (BCT1, SbtA, and NDH-1S) likely reflected a response to this perturbed ratio. Consistent with this was increased transcript abundance of genes encoding carboxysome structural proteins and carbonic anhydrase. Interestingly, the transition to pH 10 resulted in increased abundance of transcripts of photosystem II genes encoding extrinsic and low-molecular-weight polypeptides, although there was little change in photosystem I gene transcripts.

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Section: Resultsmentioning

confidence: 99%

Global Transcriptional Response of the Alkali-Tolerant CyanobacteriumSynechocystissp. Strain PCC 6803 to a pH 10 Environment

Summerfield

Sherman

2008

Appl Environ Microbiol

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“…It was described to be involved with calcium release in the cyanobacterium Synechocystis. Synechocystis MscL mutant strains, in which the mscL gene was interrupted by a kanamycin resistance gene, have more depolarized cells than the wild type in the resting state (38). We tested the MscL inhibitor GdCl 3 (18) in our strains and observed that although there was a decrease in the membrane potential, the daptomycin MIC did not change.…”

Section: Discussionmentioning

confidence: 99%

“…This channel was already described as being involved in the calcium release induced by membrane depolarization in cyanobacterial cells (38). Strains 10‫3ء‬d1 and 10‫3ء‬d1-10 presented increased levels of transcription of mscL (3.36 and 4.58, respectively).…”

Section: Downloaded Frommentioning

confidence: 99%

Serial Daptomycin Selection Generates Daptomycin-Nonsusceptible Staphylococcus aureus Strains with a Heterogeneous Vancomycin-Intermediate Phenotype

Camargo

Neoh

Cui

et al. 2008

Antimicrob Agents Chemother

109

107

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In order to better understand the mechanism of daptomycin resistance, we generated a daptomycinnonsusceptible derivative strain, strain 10‫3ء‬d1 (MIC ‫؍‬ 3.0 g/ml), by in vitro exposure of methicillinresistant Staphylococcus aureus strain N315⌬IP (MIC ‫؍‬ 0.5 g/ml) to daptomycin. We also obtained a daptomycin-susceptible phenotypic revertant strain, strain 10‫3ء‬d1-10 (MIC ‫؍‬ 1.0 g/ml), by passaging 10‫3ء‬d1 in drug-free medium for 10 days. The resultant triple-isogenic strains were analyzed for their phenotypes and gene expression by microarray analysis. No significant differences in the membrane fluidities of 10‫3ء‬d1 and 10‫3ء‬d1-10 compared to the membrane fluidity of N315⌬IP were observed. Resistant strain 10‫3ء‬d1 had the highest membrane potential, followed by strains 10‫3ء‬d1-10 and N315⌬IP. The vancomycin and teicoplanin MICs also increased. Teichoic acid genes (tagA, tagG), mprF encoding lysyl-phosphatidylglycerol, and cls encoding cardiolipin synthase were downregulated in 10‫3ء‬d1 and 10‫3ء‬d1-10. The vraF and vraG genes, which encode ATP binding cassette transporter proteins, were upregulated in 10‫3ء‬d1. The vraSR two-component regulatory system was upregulated, and electron microscopy revealed that the cell wall of 10‫3ء‬d1 was significantly thicker than that of the parental strain. Taken together, daptomycin exposure selected a daptomycin-nonsusceptible strain with a phenotype similar to that of heterogeneous vancomycin-intermediate S. aureus and a transcription profile that partially overlapped that of heterogeneous vancomycin-intermediate S. aureus.

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“…Detection of Ca 2ϩ Efflux and Membrane Potential-To prepare the Ca 2ϩ -loading cells, both the wild type and disrupted cells were incubated for 1 h with 50 mM Ca 2ϩ in buffer A (20 mM HEPES and 140 mM KCl) at various pH values (30,31). Ca 2ϩ -loaded cells were diluted 10-fold in buffer A and then transferred to the assay medium containing Ca 2ϩ indicator (2 ml) (20 mM HEPES, pH 7.2, 3 mM MgSO 4 , 27 M arsenazo III) (31).…”

Section: Construction Of Expression Vectors For Apcax and Syncax-mentioning

confidence: 99%

Isolation and Functional Characterization of Ca2+/H+ Antiporters from Cyanobacteria

Waditee

Hossain

Tanaka

et al. 2004

Journal of Biological Chemistry

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Calcium release from Synechocystis cells induced by depolarization of the plasma membrane: MscL as an outward Ca2+ channel

Abstract: Cells of the cyanobacterium Synechocystis sp. PCC 6803 are equipped with a mechanosensitive ion channel MscL that is located in their plasma membrane. However, the exact function of the channel in this freshwater cyanobacterium is unknown.

Cited by 44 publications

References 39 publications

Global Transcriptional Response of the Alkali-Tolerant CyanobacteriumSynechocystissp. Strain PCC 6803 to a pH 10 Environment

Global Transcriptional Response of the Alkali-Tolerant CyanobacteriumSynechocystissp. Strain PCC 6803 to a pH 10 Environment

Serial Daptomycin Selection Generates Daptomycin-Nonsusceptible Staphylococcus aureus Strains with a Heterogeneous Vancomycin-Intermediate Phenotype

Isolation and Functional Characterization of Ca2+/H+ Antiporters from Cyanobacteria

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