Calcium release in relation to permeability changes in toad bladder epithelium following antidiuretic hormone

Cuthbert, A. W.; Wong, Patrick

doi:10.1113/jphysiol.1974.sp010663

Cited by 35 publications

(8 citation statements)

References 27 publications

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“…There is a precedent for intracellular Ca2+ serving as a regulator of Na+ influx in other cell systems such as toad bladder (25), sea urchin eggs (26), human blood platelets (27), and MDCK cells (cultured dog kidney epithelial cells) (28). These studies and others demonstrate that intracellular Ca2+ can be regulated both by alterations in Ca2+ permeability (i.e., influx or efflux) and by changes in intracellular Ca2+ binding (i.e., redistribution or sequestration).…”

Section: Discussionmentioning

confidence: 99%

Evidence for a role of calmodulin in serum stimulation of Na+ influx in human fibroblasts.

Owen¹,

Villereal²

1982

Proc. Natl. Acad. Sci. U.S.A.

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Section: Discussionmentioning

confidence: 99%

Evidence for a role of calmodulin in serum stimulation of Na+ influx in human fibroblasts.

Owen¹,

Villereal²

1982

Proc. Natl. Acad. Sci. U.S.A.

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“…(Rasmussen, 1966(Rasmussen, , 1970Rasmussen and Goodman, 1977;Carafoli, 1980). In the amphibian urinary bladder, ADH induces a change in the cytosolic calcium ion concentration (Thorn and Schwartz, 1965;Schwartz and Walter, 1969;Rasmussen, 1970;Cuthbert and Wong, 1974;Berridge, 1975). The granular cells of the bladder epithelium are believed to be the primary site of action for ADH (DiBona et al, 1969a,b).…”

Section: Energy ( K E V )mentioning

confidence: 97%

Histochemical and elemental localization of calcium in the granular cell subapical granules of the amphibian urinary bladder epithelium

et al. 1987

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The ultrahistochemical analysis of apical granules in the epithelial cells, i.e., granular cells, of the amphibian urinary bladder using the N,N-naphthaloylhydroxylamine procedure identified the presence of calcium in these structures. Subsequent analytical microscopy employing fresh-frozen ultrathin cryosections for X-ray microanalysis of the granules further confirmed the above histochemical findings. In addition to calcium, elemental analysis indicated the presence of magnesium, phosphorus, sulfur, silicon, potassium, and chlorine either within or in close proximity to the granules. The possibility that these granules function as subcellular compartments for the uptake and storage of calcium ions, in a way similar to mitochondria, and thus function in intracellular calcium homeostasis, is discussed. Additionally, a role for this cation in the secretion of granular glycoproteins, i.e., stimulus-secretion coupling, is hypothesized.

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“…However, conflicting evidence exists, questioning this simple scheme. Both ADH and theophylline, which stimulate sodium transport, increase 45Ca efflux from intact toad bladder [12] and isolated bladder cells [8], presumably by release of intracellular Ca stores into the cytosol with subsequent efftux across the plasma membrane. Calcium ionophores have also been shown to increase sodium transport in frog skin to a similar extent as the stimulatory action of ADH [5] and in toad bladders, the inhibitory action of Ca ionophores can be reversed by ADH [37].…”

Section: Introductionmentioning

confidence: 99%

Fluorescent measurements of intracellular free calcium in isolated toad urinary bladder epithelial cells

Jacobs

Mandel

1987

J. Membrain Biol.

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Sodium-calcium exchange has been suggested to play a pivotal role in the regulation of cytosolic free calcium (Caf) by epithelial cells. Using isolated epithelial cells from the toad urinary bladder, Caf has been measured using the intracellular Ca-sensitive fluorescent dyes Fura 2 and Quin 2. Dye loading did not alter cell viability as assessed by measurements of ATP and ADP content or cell oxygen consumption. When basal Caf was examined over a wide range of cell dye content (from 0.04 to 180 nmol dye/mg protein) an inverse relationship was observed. At low dye content, Caf was 300-380 nM and, as dye content was increased, Caf progressively fell to 60 nM. Using low dye content cells, in which minimal alteration in Ca steady state would be expected, the role for plasma membrane Na-Ca exchange was examined using either medium sodium substitution or ouabain. While medium sodium substitution increased Caf, prolonged treatment with ouabain had no effect on Caf despite a clear increase in cell sodium content. The lack of effect of ouabain suggests that Na-Ca exchange-mediated Ca efflux plays a minimal role in the regulation of basal Caf. However, exchange-mediated Ca efflux may play a role in Caf regulation when cytosolic calcium is elevated.

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Calcium release in relation to permeability changes in toad bladder epithelium following antidiuretic hormone

Cited by 35 publications

References 27 publications

Evidence for a role of calmodulin in serum stimulation of Na+ influx in human fibroblasts.

Evidence for a role of calmodulin in serum stimulation of Na+ influx in human fibroblasts.

Histochemical and elemental localization of calcium in the granular cell subapical granules of the amphibian urinary bladder epithelium

Fluorescent measurements of intracellular free calcium in isolated toad urinary bladder epithelial cells

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