Calcium response in bone cells at different osteogenic stages under unidirectional or oscillatory flow

Wang, Shurong; Li, Shuna; Hu, Man; Huo, Bo

doi:10.1063/1.5128696

Cited by 12 publications

(4 citation statements)

References 45 publications

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“…Intracellular calcium is a second messenger in cells, and it plays an important role in osteogenesis [ 34 , 35 ]. Studies have shown that the concentration of intracellular calcium ion is positively associated with adhesion, migration, proliferation, and differentiation in MC3T3-E1 cells via modulation of a large variety of intracellular downstream signal targets.…”

Section: Discussionmentioning

confidence: 99%

Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound

Cai

Jiao

Quan

et al. 2021

Bioactive Materials

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Developing bioactive materials for bone implants to enhance bone healing and bone growth has for years been the focus of clinical research. Barium titanate (BT) is an electroactive material that can generate electrical signals in response to applied mechanical forces. In this study, a BT piezoelectric ceramic coating was synthesized on the surface of a TC4 titanium alloy, forming a BT/TC4 material, and low-intensity pulsed ultrasound (LIPUS) was then applied as a mechanical stimulus. The combined effects on the biological responses of MC3T3-E1 cells were investigated. Results of scanning electron microscopy, energy-dispersive X-ray spectroscopy, and X-ray diffraction showed that an uniform nanospheres -shaped BT coating was formed on TC4 substrate. Piezoelectric behaviors were observed using piezoelectric force microscopy with the piezoelectric coefficient d 33 of 0.42 pC/N. Electrochemical measures indicated that LIPUS-stimulated BT/TC4 materials could produce a microcurrent of approximately 10 μA/cm 2 . In vitro , the greatest osteogenesis (cell adhesion, proliferation, and osteogenic differentiation) was found in MC3T3-E1 cells when BT/TC4 was stimulated using LIPUS. Furthermore, the intracellular calcium ion concentration increased in these cells, possibly because opening of the L-type calcium ion channels was promoted and expression of the Ca V 1.2 protein was increased. Therefore, the piezoelectric BT/TC4 material with LIPUS loading synergistically promoted osteogenesis, rending it a potential treatment for early stage formation of reliable bone-implant contact.

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Section: Discussionmentioning

confidence: 99%

Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound

Cai

Jiao

Quan

et al. 2021

Bioactive Materials

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show abstract

“…[41]. Wang et al demonstrated that concentrations of 1.8 mM and 3.8 mM Mg 2+ signi cantly decreased calcium oscillation amplitude or frequency, while increasing the concentration of Mg 2+ from 0.8 to 1.3 mM exhibited no effect on calcium oscillation frequency [42]. The authors deduced that a high Mg 2+ environment inhibits matrix mineralization by suppressing the calcium oscillation frequency in hBMSCs.…”

Section: Discussionmentioning

confidence: 99%

Low Mg Content on Ti-Nb-Sn Alloy When in Contact with BMMSCs Promotes Improvement of its Biological Functions

Dias

Rossi

Apolonio

et al. 2021

Preprint

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Introduction: Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive.Objective: This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs).Methods: Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE).Results and discussion: The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release.

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“…[ 41 ]. Wang et al demonstrated that concentrations of 1.8 mM and 3.8 mM Mg 2+ significantly decreased calcium oscillation amplitude or frequency, while increasing the concentration of Mg 2+ from 0.8 to 1.3 mM exhibited no effect on calcium oscillation frequency [ 42 ]. The authors deduced that a high Mg 2+ environment inhibits matrix mineralization by suppressing the calcium oscillation frequency in hBMSCs.…”

Section: Discussionmentioning

confidence: 99%

Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions

Dias

Rossi

Apolonio

et al. 2021

J Mater Sci: Mater Med

View full text Add to dashboard Cite

Magnesium is a metal used in the composition of titanium alloys and imparts porosity. Due to its osteoconductive, biocompatible and biodegradable characteristics, its application in the development of biomedical materials has become attractive. This study aimed to evaluate the influence of magnesium present in porous Ti-Nb-Sn alloys, which have a low elastic modulus in adhesive, osteogenic properties and the amount of reactive intracellular oxygen species released in mesenchymal stem cells derived from bone marrow equine bone (eBMMSCs). Mechanical properties of the alloy, such as hardness, compressive strength and elastic modulus, were analyzed, as well as surface morphological characteristics through scanning electron microscopy. The evaluation of magnesium ion release was performed by atomic force spectroscopy. The biological characteristics of the alloy, when in contact with the alloy surface and with the culture medium conditioned with the alloy, were studied by SEM and optical microscopy. Confirmation of osteogenic differentiation by alizarin red and detection of ROS using a Muse® Oxidative Stress Kit based on dihydroetide (DHE). The alloy showed an elastic modulus close to cortical bone values. The hardness was close to commercial Ti grade 2, and the compressive strength was greater than the value of cortical bone. The eBMMSCs adhered to the surface of the alloy during the experimental time. Osteogenic differentiation was observed with the treatment of eBMMMSCs with conditioned medium. The eBMMSCs treated with conditioned medium decreased ROS production, indicating a possible antioxidant defense potential of magnesium release.

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Calcium response in bone cells at different osteogenic stages under unidirectional or oscillatory flow

Abstract: Human stroma and epithelium co-culture in a microfluidic model of a human prostate gland Biomicrofluidics 13, 064116 (2019);

Cited by 12 publications

References 45 publications

Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound

Improved activity of MC3T3-E1 cells by the exciting piezoelectric BaTiO3/TC4 using low-intensity pulsed ultrasound

Low Mg Content on Ti-Nb-Sn Alloy When in Contact with BMMSCs Promotes Improvement of its Biological Functions

Low Mg content on Ti-Nb-Sn alloy when in contact with eBMMSCs promotes improvement of its biological functions

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