Calcium signaling and regulation of ecdysteroidogenesis in crustacean Y-organs

Weiner, Amanda C.; Chen, Hsiang-Yin; Roegner, Megan E.; Watson, R. Douglas

doi:10.1016/j.ygcen.2021.113901

Cited by 10 publications

(1 citation statement)

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“…Unexpectedly, upregulation of both MIH and the tested Halloween genes was observed in the HA group, indicating that environmental ammonia at 20 mg/L can abolish the inhibitory activity of MIH on the transcription of the Halloween genes in juvenile P. trituberculatus . However, to date, the signaling mechanisms by which MIH regulates the Halloween genes are not well understood [ 43 ], and the MIH receptor remains unidentified [ 44 , 45 ]. Several studies in decapods have suggested that the receptors for MIH are GPCRs located in YO and that the signal is transduced by cyclic nucleotide second messengers [ 45 ].…”

Section: Discussionmentioning

confidence: 99%

Ammonia Stress Disturbs Moult Signaling in Juvenile Swimming Crab Portunus trituberculatus

Wang

Liu

Shang

et al. 2023

Biology

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Ammonia is a significant concern during hatchery culture in brachyuran species, and its accumulation may lead to abortive moulting and large-scale deaths of the early juveniles. To date, the underlying mechanism for ammonia-induced alteration of the moulting process is still unknown. In this study, we aimed to investigate the effects of ammonia on the moulting as well as the potential mechanisms in early juveniles of the swimming crab Portunus trituberculatus, an important aquaculture species in China. We evaluated the survival rate and moulting rate of the juvenile crabs (C2) and analyzed the expression pattern of the genes in key components of molt signaling during a complete moulting cycle under different concentrations of ammonia nitrogen (the control group: <0.1 mg/L; the LA group: 5 mg/L; and the HA group: 20 mg/L). The results showed that: (1) the survival rate in the LA and HA groups was lower than that in the control group at the end of the experiment, and moulting death syndrome (MDS) was only observed in the HA group; (2) the moulting rate was higher in the LA group and lower in the HA group compared to the control group; (3) consistent with the results of the moulting experiment, MIH showed decreased expression, and genes related to ecdysteroid synthesis, ecdysteroid receptors, and responsive effectors exhibited increased expression in the LA group compared to the control group; and (4) although MIH expression was upregulated, increased expression of the genes associated with ecdysteroid synthesis, ecdysteroid receptors and downstream effectors still observed in the HA group. Our results indicated that low levels of ammonia can promote moulting in juvenile swimming crabs by inhibiting the expression of MIH and activating moult signaling, whereas high levels of ammonia inhibit moulting and lead to MDS through impairing moult signaling.

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