Calculating sugar yields in high solids hydrolysis of biomass

Zhu, Yongming; Malten, Marco; Torry-Smith, Mads; McMillan, James D.; Stickel, Jonathan J.

doi:10.1016/j.biortech.2010.10.134

Cited by 61 publications

(40 citation statements)

References 8 publications

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“…Glucose yields were determined as per Zhu et al (2011), which was found fit for calculating yields due to the experiment being performed at high solids loadings, in which the density of the liquid phase and the liquid volume in the reactor would be changing during the reaction. Glucose yields were calculated as follows,…”

Section: Glucan Conversion In High Solids Fed-batch Hydrolysismentioning

confidence: 99%

“…where Y g is the glucose yield (as a fraction of 100), C g and C g;0 are concentrations of glucose (g/L), V h and V h0 , are volumes of the hydrolyzate (L), u G is the molecular weight ratio of glucose to glucan monomer (1.11), W t is the total weight of the hydrolysis assay (g), f ts;0 is the initial mass fraction of total solids, X is;0 is the initial mass fraction of insoluble solids in total solids (i.e., f is;0 =f ts;0 ), u gos is the molecular weight ratio of glucose to average monomer weight of glucose oligomers (1.08), and C gos;0 is the initial concentration of glucose oligomers (Zhu et al, 2011). All terms in Eq.…”

Section: Glucan Conversion In High Solids Fed-batch Hydrolysismentioning

confidence: 99%

See 1 more Smart Citation

A process for energy-efficient high-solids fed-batch enzymatic liquefaction of cellulosic biomass

Cardona

Tozzi²,

Karuna

et al. 2015

Bioresource Technology

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Section: Glucan Conversion In High Solids Fed-batch Hydrolysismentioning

confidence: 99%

Section: Glucan Conversion In High Solids Fed-batch Hydrolysismentioning

confidence: 99%

A process for energy-efficient high-solids fed-batch enzymatic liquefaction of cellulosic biomass

Cardona

Tozzi²,

Karuna

et al. 2015

Bioresource Technology

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“…Monomeric sugars at concentrations of 0, 10, 30, and 50 mg/l were used as standards. The overall glucan/xylan conversions from pretreatment and hydrolysis were calculated based on sugars in enzyme hydrolysis supernatant and biomass composition of the raw feedstock using a method similar to that published by Zhu et al [33].…”

Section: Feedstock Compositional Analysis and Sugar Analysismentioning

confidence: 99%

“…Concentrations of soluble sugars from both pretreatment and hydrolysis steps were used to calculate the overall glucan/xylan conversions from raw feedstock using a method published by Zhu et al [33]. Figure 2 shows glucose and xylose yields from hydrolysis of corn stover pretreated with constant nominal acid solution concentration (Fig.…”

Section: Effect Of Acid Solution Concentration and Biomass Acid Loadingmentioning

confidence: 99%

Reducing acid in dilute acid pretreatment and the impact on enzymatic saccharification

Chen

Stevens

Zhu

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Dilute acid pretreatment is a leading pretreatment technology for biomass to ethanol conversion due to the comparatively low chemical cost and effective hemicellulose solubilization. The conventional dilute acid pretreatment processes use relatively large quantities of sulfuric acid and require alkali for pH adjustment afterwards. Significant amounts of sulfate salts are generated as by-products, which have to be properly treated before disposal. Wastewater treatment is an expensive, yet indispensable part of commercial level biomass-to-ethanol plants. Therefore, reducing acid use to the lowest level possible would be of great interest to the emerging biomass-to-ethanol industry. In this study, a dilute acid pretreatment process was developed for the pretreatment of corn stover. The pretreatment was conducted at lower acid levels than the conventional process reported in the literature while using longer residence times. The study indicates that a 50% reduction in acid consumption can be achieved without compromising pretreatment efficiency when the pretreatment time was extended from 1-5 min to 15-20 min. To avoid undesirable sugar degradation and inhibitor generation, temperatures should be controlled below 170°C. When the sulfuric acid-to-lignocellulosic biomass ratio was kept at 0.025 g acid/g dry biomass, a cellulose-to-glucose conversion of 72.7% can be achieved at an enzyme loading of 0.016 g/g corn stover. It was also found that acid loading based on total solids (g acid/g dry biomass) governs the pretreatment efficiency rather than the acid concentration (g acid/g pretreatment liquid). While the acid loading on lignocellulosic biomass may be achieved through various combinations of solids loading and acid concentration in the pretreatment step, this work shows that it is unlikely to reduce acid use without undermining pretreatment efficiency simply by increasing the solid content in pretreatment reactors, therefore acid loading on biomass is indicated to be the key factor in effective dilute acid pretreatment.

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“…Ethanol fuel production from lignocellulosic materials, such as residual wood chips from the cellulose industry, is an emerging technology (Demirbas 2011;El-Zawawy et al 2011;Zhu et al 2011;Chen et al 2010;Romero et al 2010;Soccol et al 2010;Talebnia et al 2010;Tian et al 2010;Farrell et al 2006). Attaining cellulosic ethanol (bioethanol) involves two processes: hydrolysis of the cellulose in the lignocellulosic materials to fermentable reducing sugars and fermentation of the sugars to ethanol (Sun et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Saccharification of Brazilian sisal pulp: evaluating the impact of mercerization on non-hydrolyzed pulp and hydrolysis products

et al. 2012

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Cultivation of sisal, a plant with a short growth cycle, is highly productive in Brazil. This work is part of extensive research in which sisal is valued. In these studies, sisal fibers are used in the preparation of bio-based composites and in the derivatization of the pulp, including posterior preparation of films. This study aimed to examine the use of sisal pulp in the production of bioethanol, which can potentially be a high efficiency process because of the cellulose content of this fiber. A previous paper addressed the hydrolysis of sisal pulp using sulfuric acid as a catalyst. In the present study, the influence of the mercerization process on the acid hydrolysis of sisal pulp was evaluated. Mercerization was achieved in a 20% wt NaOH solution, and the cellulosic pulp was suspended and vigorously mixed for 1, 2 and 3 h, at 50°C. The previously characterized mercerized pulps were hydrolyzed (100°C, 30% H 2 SO 4 , v/v), and the results are compared with those obtained for unmercerized pulp (described in a companion paper). The starting sample was characterized by viscometry, a-cellulose content, crystallinity index and scanning electron microscopy. During the reactions, aliquots were withdrawn, and the liquor was analyzed by HPLC. The residual pulps (non-hydrolyzed) were also characterized by the techniques described for the initial sample. The results revealed that pretreatment decreases the polyoses content as well as causes a decrease of up to 23% in the crystallinity and up to 21% in the average molar mass of cellulose after 3 h of mercerization. The mercerization process proved to be very important to achieve the final target. Under the same reaction conditions (30% and 100°C, 6 h), the hydrolysis of mercerized pulp generated yields of up to 50% more glucose. The results of this paper will be compared with the results of subsequent studies obtained using other acids, and enzymes, as catalysts.

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Calculating sugar yields in high solids hydrolysis of biomass

Cited by 61 publications

References 8 publications

A process for energy-efficient high-solids fed-batch enzymatic liquefaction of cellulosic biomass

A process for energy-efficient high-solids fed-batch enzymatic liquefaction of cellulosic biomass

Reducing acid in dilute acid pretreatment and the impact on enzymatic saccharification

Saccharification of Brazilian sisal pulp: evaluating the impact of mercerization on non-hydrolyzed pulp and hydrolysis products

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