Calculation of cell production of coastal marine bacteria based on measured incorporation of [<sup>3</sup>H]thymidine1,2

Riemann, Bo; Bjørnsen, Peter Koefoed; Newell, Steven W.; Fallon, Robert D.

doi:10.4319/lo.1987.32.2.0471

Cited by 211 publications

(158 citation statements)

References 4 publications

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“…The second algorithm was outlined by Riemann et al (1987). The conversion factor is the number of bacterial cells produced over a selected time period of the experiment (essentially, the final bacterial abundance minus the initial bacterial abundance for that time period) divided by the total amount of radiolabel incorporated during that selected time period.…”

Section: Methodsmentioning

confidence: 99%

Bacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporation rates

Kirchman¹,

Hoch²

1988

Mar. Ecol. Prog. Ser.

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The thymidine and leucine methods were examined and used to estimate bacterial production in the Delaware Estuary. During growth experiments that minimized grazing on bacteria, conversion factors for both thymidine and leucine were initially high and then rapidly decreased to values lower than commonly-used factors (2.0 X 10" cells mol-' for thymidine). The low thymidine conversion factors may have been due to l3~]thymidine incorporation into protein, which was 72 % of total incorporation in untreated samples from the estuary. Addition of glucose reduced the leucine conversion factor from 7.4 to 3.6 X 1016 cells mol-' and the thymidine conversion factor from 1.53 to 0.68 X 10'' cells mol-'. Ammonium additions had no effect. The thymidine and leucine approaches gave similar estimates of bacterial production in the Delaware Bay (20 to 70 X 106 cells I-' h-'). Both methods confirmed that bacterial production was highest at about 40 to 50 km upstream of the mouth, which coincides with the peak in primary production. Bacterial production was about 30 % of primary production in most regions of the estuary, but increased to over 100 O/ O in the turbidity maximum where primary production was low. Bacterial production in the Delaware Estuary is apparently controlled by phytoplankton production in spite of large allochthonous sources of dissolved organic matter.

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Section: Methodsmentioning

confidence: 99%

Bacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporation rates

Kirchman¹,

Hoch²

1988

Mar. Ecol. Prog. Ser.

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“…Samples were subsequently filtered onto 0.2 mm cellulose nitrate filters, washed 10 times with 5% ice cold TCA and counted using liquid scintillation counting. The incorporated 3 H-thymidine was converted to cell production using a factor of 1.1 Â 10 18 cells mol À1 thymidine incorporated [Riemann et al, 1987].…”

Section: Measurements On Natural Sea Icementioning

confidence: 99%

Inorganic carbon transport during sea ice growth and decay: A carbon pump in polar seas

Rysgaard

Glud

Sejr³

et al. 2007

J. Geophys. Res.

229

353

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[1] During sea ice formation in polar areas, brine rejection increases the density in the underlying water column and thereby contributes to the formation of deep and intermediate water masses in the world ocean. Here we present evidence that dissolved inorganic carbon (TCO 2 ) is rejected together with brine from growing sea ice and that low temperatures may result in a significant change in the ratio of TCO 2 and alkalinity in Arctic sea ice compared with surface waters. Model calculations show that this sea ice-driven carbon pump affects surface water partial pressure of CO 2 significantly in polar seas and potentially sequesters large amounts of CO 2 to the deep ocean.Citation: Rysgaard, S., R. N. Glud, M. K. Sejr, J. Bendtsen, and P. B. Christensen (2007), Inorganic carbon transport during sea ice growth and decay: A carbon pump in polar seas,

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“…Bacterial cell production was determined using the 3 H-Thymidine incorporation techniques (Fuhrman & Azam, 1980). Conversion factors for bacterial production were calculated from bacterial cell number and 3 Hthymidine incorporation during bacterial growth in 1 μm pre-filtered seawater (Riemann et al, 1987) …”

Section: Materials and μEthodsmentioning

confidence: 99%

Distribution of marine viruses in the Central and South Adriatic Sea

et al. 2014

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The seasonal distribution of marine viruses and their relationship with heterotrophic bacteria in the coastal and offshore area of the central and southern Adriatic was studied. Additionally, the percentage of high (HNA) and low (LNA) nucleic acid bacteria in the total number of bacteria and the distribution of heterotrophic nanoflagellates (HNF), as a major predator of bacteria, were studied as well. Viral abundance ranged from 3.55 to 27.32 × 10 6 virus-like particles mL -1 , and was on average 25-fold higher than bacterial abundances at all investigated stations. The highest viral abundances were found at coastal stations, especially in the area influenced by the rivers Krka and Jadro, whereas the lowest values were found in the open sea and in the coastal area of the southern Adriatic. No significant difference was established in the relationship of the viruses with HNA and LNA bacterial groups. The ratio between viruses and bacteria (VBR) was higher during the colder part of the year, which coincided with lower HNF abundance and vice versa during the warmer part of the year. This suggests that HNF grazing could be more important in controlling bacterial abundance during the warmer part of the year, and viral lysis during the colder part of the year.

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Calculation of cell production of coastal marine bacteria based on measured incorporation of [³H]thymidine1,2

Abstract: This article is in Free Access Publication and may be downloaded using the “Download Full Text PDF” link at right.

Cited by 211 publications

References 4 publications

Bacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporation rates

Bacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporation rates

Inorganic carbon transport during sea ice growth and decay: A carbon pump in polar seas

Distribution of marine viruses in the Central and South Adriatic Sea

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Calculation of cell production of coastal marine bacteria based on measured incorporation of [3H]thymidine1,2

Abstract: This article is in Free Access Publication and may be downloaded using the “Download Full Text PDF” link at right.

Cited by 211 publications

References 4 publications

Bacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporation rates

Bacterial production in the Delaware Bay estuary estimated from thymidine and leucine incorporation rates

Inorganic carbon transport during sea ice growth and decay: A carbon pump in polar seas

Distribution of marine viruses in the Central and South Adriatic Sea

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Calculation of cell production of coastal marine bacteria based on measured incorporation of [³H]thymidine1,2