Calculation of induced circular dichroism of acridine orange-poly(riboadenylic acid) complexes and confirmation of their structure

Imae, Toyoko; Hayashi, Shoji; Ikeda, Shôichi

doi:10.1021/ma00169a021

Cited by 5 publications

(12 citation statements)

References 18 publications

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“…In BC, unlike the case with DAPI, the CD data does not clearly indicate the two modes of binding. But, in conjunction with spectrophotometric data, the two modes are clearly deliniated, independantly moreover, the features of the extrinsic CD profiles actually observed are quite complex as with the case of acridine orange (58,59). It appears, therefore, that in the interaction between BC and DNAs, the geometrical interrelation between the alkaloid and the bases, the conformation of the polynucleotide as governed by factors such as orientation, depth of intercalation site, electrostatic contribution etc.…”

Section: Discussionmentioning

confidence: 91%

“…There are several pieces of information in the literature that correlates the binding modes tothe orgin of extrinsic CD in dye-DNA interactions (34,(54)(55)(56)(57)(58)(59)(60). These have resulted from extensive studies on intercalators like proflavine (54), ethidium bromide (55)(56)(57).…”

Section: Discussionmentioning

confidence: 99%

“…These have resulted from extensive studies on intercalators like proflavine (54), ethidium bromide (55)(56)(57). acridine orange (58,59) aminoacridines (36) and DAPI (60). Based on studies of Dalgleish and co-workers (35,54) and Blake and Peacocke (61) two types of mechanisms have been proposed for the induction of optical activity in symmetrical dyes bound to DNAs.…”

Section: Discussionmentioning

confidence: 99%

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Circular Dichroism Studies of the Structure of DNA Complex with Berberine

Debnath

Kumar

Maiti

1991

Journal of Biomolecular Structure and Dynamics

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The binding of the benzodioxolo-benzoquinolizine alkaloid, berberine chloride to natural and synthetic DNAs has been studied by intrinsic and extrinsic circular dichroic measurements. Binding of berberine causes changes in the circular dichroism spectrum of DNA as shown by the increase of molar ellipticity of the 270nm band, but with very little change of the 240nm band. The molar ellipticity at the saturation depends strongly on the base composition of DNA and also on salt concentration, but always larger for the AT rich DNA than the GC rich DNA. The features in the circular dichroic spectral changes of berberine-synthetic DNA complexes were similar to that of native DNA, but depends on the sequence of base pairs. On binding to DNA and polynucleotides, the alkaloid becomes optically active. The extrinsic circular dichroism developed in the visible absorption region (300-500nm) for the berberine-DNA complexes shows two broad spectral bands in the regions 425-440nm and 340-360nm with the maximum varying depending on base composition and sequence of DNA. While the 425nm band shows less variation on the binding ratio, the 360nm band is remarkably dependent on the DNA/alkaloid ratio. The generation of the alkaloid associated extrinsic circular dichroic bands is not dependent on the base composition or sequence of base pairs, but the nature and magnitude of the bands are very much dependent on these two factors and also on the salt concentration. The interpretation of the results with respect to the modes of the alkaloid binding to DNA are presented.

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Section: Discussionmentioning

confidence: 91%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Circular Dichroism Studies of the Structure of DNA Complex with Berberine

Debnath

Kumar

Maiti

1991

Journal of Biomolecular Structure and Dynamics

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“…It is also interesting to note that both the 290 and 600 nm exciton bands are more intense in the higher ionic strength medium at pH 7 .8, suggesting that the bound dye molecules are more effectively coupled. An antiparallel arrangement of dye molecules is the generally accepted geometry for dimers of the dye (7,12).…”

Section: Discussionmentioning

confidence: 99%

“…For acridine orange-poly(rA) complexes, Imae et al, from extensive theoretical and experimental studies of induced circular dichroism (ICD) profiles, proposed four different binding modes depending upon the pH of the medium and phosphate to dye (P /D) ratio ( 6,7). In addition to external binding, they suggested different modes of intercalation -isolated intercalation in which electronic transitions of the bound dye molecules cannot couple, intercalation which promotes coupling between the bound dye molecules and yet another mode in which an unbound monomeric dye molecule stacks on a partially intercalated monomer.…”

mentioning

confidence: 99%

Spectroscopic Studies on the Binding of Methylene Blue to poly(riboadenylic acid)

Antony¹,

Atreyi²,

Rao³

1993

Journal of Biomolecular Structure and Dynamics

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The binding of methylene blue (MB) to poly(rA) was studied using UV and CD spectroscopy at neutral and acidic pH, in which poly(rA) exists as a single strand and a duplex respectively. UV spectroscopic studies and analysis of equilibrium binding data show that salt increases the cooperativity parameter but reduces the binding between the cationic dye and the negatively charged phosphate groups. The binding constant for the single strand-MB complex decreased from 1.15 x 10(5) M-1 to 7.62 x 10(3) M-1 while the cooperativity parameter increased from 9 to 71, when salt concentration was increased from 1 mM to 200 mM. At neutral pH, changes in the induced circular dichroism of MB-poly(rA) complex with ionic strength show predominance of electrostatic binding to the phosphate group externally on the poly(rA) chain; a partial intercalation between the adenine bases is also indicated. A red shift in the visible absorption band and the ICD profile of the double stranded poly (rA)-MB complex suggest an electronically coupled intercalation mode for the dye binding at pH 4.2.

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Bildungsweise, Strukture und Anwendungsmöglichkeiten von Komplexen zwischen Polyelektrolyten und ionischen Farbstoffen (Literaturübersicht) Teil II: Komplexe zwischen anionischen Polymeren und kationischen Farbstoffen

Dawydoff¹,

Linow²,

Philipp³

1991

Acta Polymerica

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Bildungsweise und Struktur von Komplexen zwischen anionischen Polymeren und kationischen Farbstoffen werden beschrieben, wobei sowohl synthetische Polyelektrolyte als auch saure Polysaccharide und Peptide betrachtet werden und als FarbstoMiomponente Acridinorange und Methylenblau im Mittelpunkt stehen. Auf einige praktische Anwendungsmoglichkeiten von Polyelektrolyt-Farbstoff-Komplexen sowie auf die Bindungsarten zwischen den Komplexbestandteilen wird eingegangen. Formation. structure and application of complexes of polyelectrolytes and ionic dyes (review) Part 11. Complexes between anionic polymers and cationic dyesMode of formation and structure of complexes between anionic polyelectrolytes and cationic dyes are surveyed, considering synthetic anionic polymers as well as acid polysaccharides and polypeptides, and centering on Acridine-orange and Methylenblue as the dye components. Finally, some areas of application of polyelectrolyte-dye complexes are discussed, and the possible modes of component binding in these complexes are summarized.

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Calculation of induced circular dichroism of acridine orange-poly(riboadenylic acid) complexes and confirmation of their structure

Cited by 5 publications

References 18 publications

Circular Dichroism Studies of the Structure of DNA Complex with Berberine

Circular Dichroism Studies of the Structure of DNA Complex with Berberine

Spectroscopic Studies on the Binding of Methylene Blue to poly(riboadenylic acid)

Bildungsweise, Strukture und Anwendungsmöglichkeiten von Komplexen zwischen Polyelektrolyten und ionischen Farbstoffen (Literaturübersicht) Teil II: Komplexe zwischen anionischen Polymeren und kationischen Farbstoffen

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