Calculation of the composition of sample zones in capillary zone electrophoresis I. Mathematical model

Beckers, JL Jozef

doi:10.1016/0021-9673(94)01043-e

Cited by 36 publications

(27 citation statements)

References 9 publications

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“…Recently [12], we found analytic solutions for the transport of monofunctional acids or bases in buffers based on monofuncResults of the two approaches are numerically the same. Together with the results cited above [8][9][10][11][12] they yield a common result of general importance. In this work we will restrict the discussion to cases of relatively small overload, such that extreme effects as, e.g., peak splitting, described and explained by Ermakov et al [13], do not occur.…”

Section: The Electromigration Dispersion Emd Constantsupporting

confidence: 65%

“…The effect of pH Changes has received little attention; recently, Beckers [10] applied a numerical procedure to obtain insight into this phenomenon. In our own work it has become possible to describe and predict this effect in two ways: (a) (b)…”

Section: The Electromigration Dispersion Emd Constantmentioning

confidence: 99%

“…The description of the triangular peak shape obtained under overload conditions is a longstanding result of the theoretical efforts in electrophoresis [8][9][10]. We shall therefore give only a brief account, just to put the derivations in our symbols.…”

Section: Peak Shape Under Emd Conditionsmentioning

confidence: 99%

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Optimum conditions for preparative operation of capillary zone electrophoresis

Cifuentes

Kok

et al. 1995

Journal of Chromatography A

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Optimum conditions for preparative operation of capillary zone electrophoresisCifuentes, A.; Xu, X.; Kok, W.T.; Poppe, H. General rightsIt is not permitted to download or to forward/distribute the text or part of it without the consent of the author(s) and/or copyright holder(s), other than for strictly personal, individual use, unless the work is under an open content license (like Creative Commons). Disclaimer/Complaints regulationsIf you believe that digital publication of certain material infringes any of your rights or (privacy) interests, please let the Library know, stating your reasons. In case of a legitimate complaint, the Library will make the material inaccessible and/or remove it from the website. Please Ask the Library: http://uba.uva.nl/en/contact, or a letter to: Library of the University of Amsterdam, Secretariat, Singel 425, 1012 WP Amsterdam, The Netherlands. You will be contacted as soon as possible. AbstractThe possibilities to use capillary zone electrophoresis (CZE) as a preparative tool have been studied, mostly from a theoretical point of view. The preparative performance of CZE is quantitated by the production rate, the amount of analyte that can be purified per unit of time. The production rate available with CZE is shown to depend in the first place on the chemical characteristics of the analyte-buffer combination. A figure of merit can be defined for a buffer system, describing its susceptibility to analyte overloading. Methods to find an optimal bufer system are referred to. It is shown that the loadability of the system is inversely proportional to the plate number required for the separation.A second important factor for the maximum production rate is shown to be the thermal management of the instrumental system. The average increase of the solution temperature and the non-uniform migration velocity by temperature differences within the solutions are discussed. It is shown that in practical cases the temperature rise of the solutions will be the main limiting factor when high fields are used. With low fields, which in general give a higher production rate, siphoning of the solution becomes the dominant limitation for the production rate.With cylindrical capillaries the highest production rate that can be obtained under practical conditions is in the order of 5.10 -13 mol s -1. Experimental and theoretical results indicate that with rectangular capillaries the production rate can be increased by a factor of three.

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Section: The Electromigration Dispersion Emd Constantsupporting

confidence: 65%

Section: The Electromigration Dispersion Emd Constantmentioning

confidence: 99%

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Optimum conditions for preparative operation of capillary zone electrophoresis

Cifuentes

Kok

et al. 1995

Journal of Chromatography A

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“…For negatively charged sample components, these equations are as follows (see Refs. [5][6][7] for a complete description of the equations).…”

Section: Theorymentioning

confidence: 99%

“…For steady-state processes, such as isotachophoresis (ITP), these models are generally known [1][2][3][4], but they also can be applied for nonsteady-state processes. In Part I [5] of this series of papers, it has been described already that non-steadystate electrophoretic processes can be treated by a repeated application of a steady-state model and a mathematical model is given for the calculation of all parameters in sample zones in capillary zone electrophoresis (CZE). In this model, sample zones in zone electrophoresis are divided into segments with varying sample concentrations and by solving the set of equations describing the relationships between all parameters of two adjacent peak segments, all pa-rameters of a specific segment can be calculated from those of the preceding segment (with lower analyte concentration), starting from the composition of the background electrolyte (BGE).…”

Section: Introductionmentioning

confidence: 99%