Calf production from vitrified bovine sexed embryos following in‐straw dilution

Akiyama, Kiyoshi; Kobayashi, Jun; Sato, Yoshihiro; Sata, Ryuichi; Ohashi, Megumi; Sasaki, Emi; Oda, Yorimasa; Ogawa, Yoshio; Ueda, Shuji; Nabenishi, Hisashi; Matoba, S.

doi:10.1111/j.1740-0929.2010.00763.x

Cited by 15 publications

(5 citation statements)

References 20 publications

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“…Lopes et al (2001) obtained comparable pregnancy rates for bisected (60%) and intact embryos (61%), while Lopatarova et al (2008) showed a 56.5% pregnancy rate after the transfer of sexed demi-embryos. Akiyama et al (2010) showed that the pregnancy rate after transferring vitrified sexed bovine embryos was of 38.7% and 34.8%. The biopsy technique used in our study involved the aspiration of several blastomeres (25 to 30% of the inner cell mass), as it was performed by slicing the embryo with a microblade.…”

Section: Resultsmentioning

confidence: 99%

Sexing bovine pre-implantation embryos using the polymerase chain reaction: A model for human embryo sexing

Cenariu¹

2012

Afr. J. Biotechnol.

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The paper aims to present a bovine model for human embryo sexing. Cows were super-ovulated, artificially inseminated and embryos were recovered 7 days later. Embryo biopsy was performed; DNA was extracted from blastomeres and amplified using bovine-specific and bovine-Y-chromosomespecific primers, followed by agarose gel electrophoresis. Embryos were then transferred to synchronized recipients and PCR predicted sex was compared to the actual sex of the newborn calves. Results showed a 39% pregnancy rate and a 95.65% accuracy of the sexing method. The sexing method can also be applied to human embryos, using different primers, designed for human DNA.

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Section: Resultsmentioning

confidence: 99%

Sexing bovine pre-implantation embryos using the polymerase chain reaction: A model for human embryo sexing

Cenariu¹

2012

Afr. J. Biotechnol.

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“…Although the pregnancy rate of the biopsied embryos was the same as that of the intact embryos, the pregnancy rates of both the frozen sexed embryos and the sexed embryos obtained from low-quality embryos were lower [3]. The biopsy-sexing of the low-quality embryos was inadequate for embryo manipulation since it could potentially reduce the viability of the embryos after ET [2, 4]. Moreover, the amplification of the male-specific DNA sequences of embryos is not financially optimal as approximately over 50% of the recovered embryos are of the undesired sex and are therefore wasted.…”

Section: Discussionmentioning

confidence: 99%

“…MOET is used with sexing equipment in dairy management for the systematic production of females. Diagnostic embryo sexing from a biopsy sample [2,3,4] and MOET with X-sorted semen [5, 6] has been previously used in the dairy farming industry.…”

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confidence: 99%

Comparative evaluation of the cost and efficiency of four types of sexing methods for the production of dairy female calves

Obuchi

Osada

Ozawa

et al. 2019

Journal of Reproduction and Development

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This study was conducted to evaluate and compare the economic benefits of different embryo sexing methods, based on the cost per female dairy calf produced. Female calves were produced from four kinds of female embryos: (1) those collected from superstimulated donors at 7–8 days after artificial insemination (AI) with X-sorted semen; (2) those sex-determined by loop-mediated isothermal amplification assay of a biopsy sample of embryos collected from superstimulated donors after AI with conventional unsorted semen; (3) those obtained by in vitro embryo production (IVEP), using X-sorted semen and in vitro -matured oocytes collected from donors by ovum pick-up (OPU); and (4) those obtained by IVEP, using X-sorted semen and oocytes collected by OPU after dominant follicle ablation and follicle growth stimulation of the donors. The respective productivities of female calves per technical service and the total production cost per female calf of each sexing method were compared. The production cost per female calf (66,537 JPY), as calculated from the number of female calves per service (1.30), pregnancy rate of transfer (42.9%), rate of female calves obtained (92.9%), and total cost of the method (56,643 JPY plus embryo transfer fee), was less for IVEP with X-sorted semen and follicular growth-stimulated (FGS) oocytes than for the other groups (P < 0.05). The results demonstrate that embryo production with X-sorted semen and FGS oocytes provides a more efficient method for producing female calves than the other embryo sexing methods.

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“…It has also been reported that bovine embryo vitrification and subsequent cryoprotectants dilution with the one‐step in‐straw method was comparable to that of the slow freezing method (Sano et al., ). In addition, the vitrification methods were also useful for sex‐determined embryos with low cryotolerance (Akiyama et al., ; Inaba et al., ). The vitrification and cryoprotectants dilution used in the present study might be feasible for embryos with extremely low cryotolerance.…”

Section: Discussionmentioning

confidence: 99%

Vitrification for bovine embryos with low‐quality grade

Tajimi

Yamazaki

Oike

et al. 2018

Animal Science Journal

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This study was conducted to examine the utility of vitrification for bovine embryos with low-quality grade, and simple cryoprotectants dilution method for practitioners. In Experiment 1, survival of frozen embryos was compared with that of vitrified embryos using minimum volume cooling (MVC). Then, vitrified embryos were used to confirm the optimum sucrose concentration in Experiment 2. The survival rates of embryos that had been vitrified following diluted cryoprotectants with the one-step in-straw method were compared with those of fresh control embryos in Experiment 3. Frozen-thawed or vitrified-warmed blastocysts were cultured with TCM-199 supplemented with 100 μmol/L beta-mercaptoethanol +5% fetal bovine serum at 38.5°C in an atmosphere of 5% CO in air, their survival after 24 hr were compared. The development to term of fair quality in vivo embryos after vitrification was examined in Experiment 4. Results show that survival rates of frozen-thawed embryos were lower (p < .05) than that of vitrified-warmed ones. When vitrified embryos were warmed in 0.3 mol/L sucrose in straws, their survival rate was 100%. The total cell numbers of vitrified-warmed embryos were comparable to those of fresh control embryos. The six calves from 13 vitrified embryos were delivered in Experiment 4. These results indicate that MVC vitrification following one-step cryoprotectants dilution is utilized to preserve low-quality bovine embryos.

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Calf production from vitrified bovine sexed embryos following in‐straw dilution

Cited by 15 publications

References 20 publications

Sexing bovine pre-implantation embryos using the polymerase chain reaction: A model for human embryo sexing

Sexing bovine pre-implantation embryos using the polymerase chain reaction: A model for human embryo sexing

Comparative evaluation of the cost and efficiency of four types of sexing methods for the production of dairy female calves

Vitrification for bovine embryos with low‐quality grade

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