Calibrating analytical ultracentrifuges

Zhao, Huaying; Nguyen, Ai; To, Samuel C.; Schuck, Peter

doi:10.1007/s00249-020-01485-2

Cited by 7 publications

(4 citation statements)

References 33 publications

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“…Maximum entropy regularization with p = 0.9 was applied. Scaling factors for transformation of experimental s -values to the reference values in water at 20 °C were calculated with the software SEDNTERP 3.0.3, kindly provided by Dr John Philo, after AUC calibration ( 26 ).…”

Section: Methodsmentioning

confidence: 99%

The intrinsic kinase activity of BRD4 spans its BD2-B-BID domains

Weissman

Singh

Devaiah

et al. 2021

Journal of Biological Chemistry

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This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

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Section: Methodsmentioning

confidence: 99%

The intrinsic kinase activity of BRD4 spans its BD2-B-BID domains

Weissman

Singh

Devaiah

et al. 2021

Journal of Biological Chemistry

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“…Furthermore, this same shift is found for a second Biogen Optima and has been observed on other Optima instruments (unpublished information), suggesting there is a common origin for the problem. Two possibilities are: 1) differences in radial calibration [12][13][14] or 2) differences in how ω 2 t is calculated between these two detectors. At present, we do not know which detector provides more accurate S values, but Optima users should be aware of and test for this possibility.…”

Section: Technical Notementioning

confidence: 99%

Using absorbance detection for hs-SV-AUC characterization of adeno-association virus

Larson,

Bou-Assaf,

Laue

et al. 2024

Preprint

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“…The working group entitled 'Optimization of data quality' has been particularly popular and has focussed primarily on helping cross-laboratory teams (i) develop standard samples (Birchenough et al 2021) which will be used in future training workshops and benchmarking studies, (ii) establish standard operating procedures (SOP's), (iii) carry out benchmarking studies and iv) define procedures for the optimisation of sample quality. The network has already generated SOP's across many instruments and methods, including enzymatic Activity inhibition assays (Smirnovienė et al 2021), calibration of analytical ultracentrifuges (Zhao et al 2021), isothermal titration calorimetry (Bastos and Velazquez-Campoy 2021), fluorescent thermal shift assays (Kazlauskas et al 2021), microscale thermophoresis (MST) experiments (Sedivy 2021), Switchsense DRX instruments (Müller-Landau and Fernandez Varela 2021) and mass photometry (Wu and Piszczek 2021). There have been two extremely successful benchmarking studies, one using the standard samples developed by the network examining the reproducibility and accuracy of MST instrumentation (López-Méndez et al 2021a) and a multi-laboratory benchmark of ITC instrumentation using EDTA metal chelation (Velazquez-Campoy et al 2021).…”

Section: Scientific Outputs Of the Mobieu Cost Actionmentioning

confidence: 99%

Community-building and promotion of technological excellence in molecular biophysics: the ARBRE–MOBIEU network

England

Jowitt

2021

Eur Biophys J

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The interface between Physics and Biology (Biophysics) has been, for centuries, a fantastically rich environment for scientific and technological innovation.Molecular-scale biophysics is the study of biological systems at an intermediate level between atomic-resolution structural descriptions and cellular-scale observations. It sits at the crossroads of several areas of expertise (Fig. 1), and thus holds a strategic position, critical to cellular, molecular and structural biology, as well as to biomedicine, bio-production and biotechnology. It addresses essential questions on how active biomolecular assemblies form and function. These include insights into their architecture, folding, stability and dynamics, as well as into the energy and kinetics of their interactions, both at ensemble and single-event levels. It is a strongly interdisciplinary field involving physicists, biologists, chemists, as well as medical, bioinformatics and materials scientists.The last decade has witnessed a remarkable expansion in the variety of molecular-scale biophysics technologies, utilising an ever-increasing number of physical phenomena to monitor molecular changes that allow scientists to dissect molecular behaviour and to answer biological problems. These technologies include hydrodynamics, advanced spectroscopies, calorimetry, fast and ultra-fast real-time bio-sensing, native mass spectrometry, and single-molecule

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Calibrating analytical ultracentrifuges

Cited by 7 publications

References 33 publications

The intrinsic kinase activity of BRD4 spans its BD2-B-BID domains

The intrinsic kinase activity of BRD4 spans its BD2-B-BID domains

Using absorbance detection for hs-SV-AUC characterization of adeno-association virus

Community-building and promotion of technological excellence in molecular biophysics: the ARBRE–MOBIEU network

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