The discrete element method (DEM) has been widely applied as a vital auxiliary technique in the design and optimization processes of agricultural equipment, especially for simulating the behavior of granular materials. In this study, the focus is placed on accurately calibrating the simulation contact parameters necessary for the V7 potato minituber seed DEM simulation. Firstly, three mechanical tests are conducted, and through a combination of actual tests and simulation tests, the collision recovery coefficient between the seed and rubber material is determined to be 0.469, the static friction coefficient is 0.474, and the rolling friction coefficient is 0.0062. Subsequently, two repose angle tests are carried out by employing the box side plates lifting method and the cylinder lifting method. With the application of the response surface method and a search algorithm based on Matlab 2019, the optimal combination of seed-to-seed contact parameters, namely, the collision recovery coefficient, static friction coefficient, and rolling friction coefficient, is obtained, which are 0.500, 0.476, and 0.043, respectively. Finally, the calibration results are verified by a seed-falling device that combines collisions and accumulation, and it is shown that the relative error between the simulation result and the actual result in the verification test is small. Thus, the calibration results can provide assistance for the design and optimization of the potato minituber seed planter.