Calibration

doi:10.1002/9780470442876.ch8

Cited by 2 publications

(1 citation statement)

References 29 publications

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“…Ferulic acid (194 Da) and catechol (110 Da) were used as low molecular weight standards. The calibration curves provided the following relations between molecular weight (MW) and retention time (RT): Weight-averaged (Mw) and number-averaged (Mn) molecular weights and polydispersity ( P = Mw/Mn) were calculated as described elsewhere . A Unipoint Gilson Software was used to record and elaborate the chromatograms, while the calculations of Mw, Mn, and P have been performed with the Origin software (v. 9.1, Originlab).…”

Section: Methodsmentioning

confidence: 99%

Molecular Characterization of Extracts from Biorefinery Wastes and Evaluation of Their Plant Biostimulation

Savy¹,

Mazzei²,

Δρόσος³

et al. 2017

ACS Sustainable Chem. Eng.

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Biorefinery residues from non-food biomasses are promising sources of sustainable agrochemicals. The molecular properties of water-soluble extracts from ligno-cellulosic biomass pretreated first by steam-explosion and then by enzymatic hydrolyses at different buffer doses, were assayed for bioactivity on maize. 13 C and 31 P nuclear magnetic resonance (NMR) spectra showed that extracts varied in phenolic and carboxyl content, while high performance size exclusion chromatography and diffusion ordered spectroscopy NMR revealed that Ox-BYP 1 obtained from wastes treated with a greater buffer dose contained small-sized molecules associated in apparently large metastable aggregates. Ox-BYP 2 separated from wastes treated with smaller buffer concentrations showed a more stable conformation. Both hydrolysates revealed a positive dose-dependent bioactivity toward maize growth. Ox-BYP 1 promoted plant fresh and dry weights and root length at 10 and 100 ppm but decreased seedling growth at 1 ppm. Instead, Ox-BYP 2 increased the whole plant growth at all assayed concentrations. Their different biostimulation effects were attributed to the toxicity of easily bioaccessible lignin-derived phenolics at small concentrations of Ox-BYP 1, which was removed by molecular selfassembly at greater concentrations. Conversely, the more strongly associated Ox-BYP 2 exerted a positive bioactivity even at small doses. The bioactivity of extracts from biorefinery wastes appeared to depend on molecular composition and, in turn, on waste pretreatments.

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Section: Methodsmentioning

confidence: 99%