Calibration-Less Sizing and Quantitation of Polymeric Nanoparticles and Viruses with Quartz Nanopipets

Terejánszky, Péter; Makra, István; Fürjes, Péter; Gyurcsányi, Róbert E.

doi:10.1021/ac500184z

Cited by 63 publications

(90 citation statements)

References 69 publications

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“…Method 1 assumes that the ratio between the outer and inner diameter of a nanopipette remains constant to that at the nanopipette opening, 13,41,52 and, as such, the inner nanopipette angle can be calculated by using the relationship:…”

Section: Evaluation Of Existing Methods For Nanopipette Characterizationmentioning

confidence: 99%

“…Nanoelectrodes can also be fabricated by electrochemically plating nanopipettes with a variety of different metals. 12 Applications of nanopipettes include as tools for sizing, counting, [13][14][15][16][17] and sequencing of single particles or molecules. [18][19][20] These applications use the changes in ionic current through the end of the nanopipette (with an applied bias), as a single entity passes through the end of the probe, to provide diagnostic information.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Nanopipettes

et al. 2016

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Nanopipettes are widely used in electrochemical and analytical techniques as tools for sizing, sequencing, sensing, delivery and imaging. For all of these applications, the response of a nanopipette is strongly affected by its geometry and surface chemistry. As the size of nanopipettes becomes smaller, precise geometric characterization is increasingly important, especially if nanopipette probes are to be used for quantitative studies and analysis. This contribution highlights the combination of data from voltage-scanning ion conductivity experiments, transmission electron microscopy (TEM) and finite element method (FEM) simulations to fully characterize nanopipette geometry and surface charge characteristics, with an accuracy not achievable using existing approaches. Indeed, it is shown that presently used methods for nanopipette characterization can lead to highly erroneous information on nanopipettes. The new approach to characterization further facilitates high-level quantification of the behavior of nanopipettes in electrochemical systems, as demonstrated herein for a scanning ion conductance microscope (SICM) setup.

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Section: Evaluation Of Existing Methods For Nanopipette Characterizationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Nanopipettes

et al. 2016

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“…The Gyurcsányi group [75] used resistive-pulse sensing with quartz nanopipettes for sizing and quantitation of polymeric NPs and polioviruses. This approach was validated using the suspensions of monodisperse latex nanoparticles as well as mixtures of closely sized NP dispersions.…”

Section: (B) Liposomes/vesicles/virusesmentioning

confidence: 99%

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Wang

Mirkin

2017

Proc. R. Soc. A.

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Along with more prevalent solid-state nanopores, glass or quartz nanopipettes have found applications in resistive-pulse and rectification sensing. Their advantages include the ease of fabrication, small physical size and needle-like geometry, rendering them useful for local measurements in small spaces and delivery of nanoparticles/biomolecules. Carbon nanopipettes fabricated by depositing a thin carbon layer on the inner wall of a quartz pipette provide additional means for detecting electroactive species and fine-tuning the current rectification properties. In this paper, we discuss the fundamentals of resistivepulse sensing with nanopipettes and our recent studies of current rectification in carbon pipettes.

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“…This sensing concept with single species detection capability has been studied for the determination of a wide variety of targets ranging from small ions [6] up to nanoparticles [6][7][8][9][10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Nanoparticle displacement assay with electrochemical nanopore-based sensors

Lautner

Plesz

Jágerszki³

et al. 2016

Electrochemistry Communications

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Please cite this article as: Gergely Lautner, Mónika Plesz, Gyula Jágerszki, Péter Fürjes, Róbert E. Gyurcsányi, Nanoparticle displacement assay with electrochemical nanopore-based sensors, Electrochemistry Communications (2016Communications ( ), doi: 10.1016Communications ( /j.elecom.2016 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. A C C E P T E D M A N U S C R I P T ACCEPTED MANUSCRIPT Abstract:The proof of concept of a nanoparticle displacement assay that enables the use of large diameter nanopores for the detection of targets of smaller molecular dimensions is presented. We hypothesized that an inherent signal amplification should arise from the selective displacement of nanoparticles preloaded in a nanopore by a much smaller molecular target. The method is demonstrated using peptide nucleic acid (PNA)-functionalized gold nanopore arrays in which short DNA-modified gold nanoparticles are anchored by weak interaction. Complementary microRNAs are detected via the resistance change caused by competitive displacement of nanoparticles from the PNA-functionalized nanopores.

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Calibration-Less Sizing and Quantitation of Polymeric Nanoparticles and Viruses with Quartz Nanopipets

Cited by 63 publications

References 69 publications

Characterization of Nanopipettes

Characterization of Nanopipettes

Resistive-pulse and rectification sensing with glass and carbon nanopipettes

Nanoparticle displacement assay with electrochemical nanopore-based sensors

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