Under constant temperature conditions, air relative humidity variations affect hygroscopic objects, such as wood, modifying their moisture content and provoking, at low values, damages and fractures. The parameters (amplitude, counts, and energy) derived from the acoustic emission non-destructive technique are calibrated with respect to equilibrium moisture content values using 14 samplings of Pinus sylvestris. The experimental procedure uses multi-technique approaches involving a universal testing machine, digital image correlation, and acoustic emissions, and notes that the three parameters of acoustic emission strictly depend on the equilibrium moisture content. For a better interpretation, a statistical approach is applied to model the equilibrium moisture content variations radially and longitudinally. Amplitude, counts, and energy are calibrated as a function of the equilibrium moisture content, indicating that all three parameters are necessary to have an integral vision of the conservation of a wooden material. Moreover, the shape of the macro-fractures that occur at the surface can be associated with the level of moisture or dryness of the sample. Finally, the proposed method can be used in situ because, through acoustic emission monitoring, it is possible to quantify the fingerprint of the state of conservation of a material.