Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish

Andrewartha, Sarah J.; Munns, Suzanne L.; Edwards, Ashley

doi:10.1093/conphys/cow006

Cited by 16 publications

(14 citation statements)

References 26 publications

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“…Algunos estudios han demostrado que los dispositivos ELA son bastante exactos con sangre venosa humana (Hiscock et al, 2015;Rosenblit et al, 1999;von Schenck et al, 1986) y su uso, por ejemplo en el caso de HemoCue 201+1 analyzer (HemoCue Inc.), se ha ido incrementando en medicina veterinaria y estudios en vida silvestre de diferentes grupos de vertebrados (aves: Ishtiaq y Barve, 2018; Milenkaya et al, 2013; Minias, https://doi.org/10. 22201/ib.20078706e.2019.90.2848 2016; anfibios: Hopkins et al, 2106; peces y reptiles: Andrewartha et al, 2016). Sin embargo, se recomienda que al usar un hemoglobinómetro portátil para estudios de vida silvestre en campo, éste ofrezca la corrección de la turbidez a 880 nm (p.…”

Section: Discussionunclassified

Uso de hemoglobinómetro como alternativa para la medición de hemoglobina y hematocrito en muestras de aves

2019

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Section: Discussionunclassified

Uso de hemoglobinómetro como alternativa para la medición de hemoglobina y hematocrito en muestras de aves

2019

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“…Haematocrit was calculated as the percentage of packed red cell volume in whole blood following centrifugation at ~ 4000 RCF for 4 min using a microhaematocrit centrifuge (SpinCrit, USA). Blood [Hb] determined by the HemoCue (following 7 min of incubation in the microcuvette; see Clark et al (2008)) was adjusted using a calibration equation for Atlantic salmon blood (Andrewartha et al , 2016). Mean corpuscular haemoglobin concentration (MCHC) was calculated from [Hb] and haematocrit using Equation 1.\documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{upgreek} \usepackage{mathrsfs} \setlength{\oddsidemargin}{-69pt} \begin{document} }{}\begin{equation*} \mathrm{MCHC}=\frac{\left[\mathrm{Hb}\right]}{\left(\mathrm{Haematocrit}\div 100\right)} \end{equation*}\end{document}…”

Section: Methodsmentioning

confidence: 99%

Hypoxia during incubation does not affect aerobic performance or haematology of Atlantic salmon (Salmo salar) when re-exposed in later life

Wood

Andrewartha

Elliott

et al. 2019

Conservation Physiology

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Hypoxia in aquatic ecosystems is becoming increasingly prevalent, potentially reducing fish performance and survival by limiting the oxygen available for aerobic activities. Hypoxia is a challenge for conserving and managing fish populations and demands a better understanding of the short- and long-term impacts of hypoxic environments on fish performance. Fish acclimate to hypoxia via a variety of short- and long-term physiological modifications in an attempt to maintain aerobic performance. In particular, hypoxia exposure during early development may result in enduring cardio-respiratory modifications that affect future hypoxia acclimation capacity, yet this possibility remains poorly investigated. We incubated Atlantic salmon (Salmo salar) in normoxia (~100% dissolved oxygen [DO, as percent air saturation]), moderate hypoxia (~63% DO) or cyclical hypoxia (100–25% DO daily) from fertilization until 113 days post-fertilization prior to rearing all groups in normoxia for a further 8 months. At ~11 months of age, subsets of each group were acclimated to hypoxia (50% DO) for up to 44 days prior to haematology, aerobic metabolic rate and hypoxia tolerance measurements. Hypoxia exposure during incubation (fertilization to 113 days post-fertilization) did not affect the haematology, aerobic performance or hypoxia tolerance of juvenile salmon in later life. Juveniles acclimated to hypoxia increased maximum aerobic metabolic rate and aerobic scope by ~23 and ~52%, respectively, when measured at 50% DO but not at 100% DO. Hypoxia-incubated juveniles also increased haematocrit and haemoglobin concentration but did not affect acute hypoxia tolerance (critical oxygen level and DO at LOE). Thus, while Atlantic salmon possess a considerable capacity to physiologically acclimate to hypoxia by improving aerobic performance in low oxygen conditions, we found no evidence that this capacity is influenced by early-life hypoxia exposure.

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“…While these validations indicate true limitations for use in some species, there are circumstances where adjustments can be made to allow for biologically accurate results. For example, Andrewartha et al (2016) found that while the HemoCue point-of-care device overestimated haemoglobin concentration in both blue-tongued skinks ( Tiliqua nigrolutea ) and Atlantic salmon ( Salmo salar ), calibration equations can rectify this issue. Overall, we anticipate continued interest in point-of-care devices over the coming years, and we believe the studies accumulating in ‘Conservation Physiology’ can provide excellent road maps for completing the necessary intra- and inter-specific tool validations.…”

Section: Where Are Researchers Placing Emphasis For Tool Development?mentioning

confidence: 99%

The conservation physiology toolbox: status and opportunities

Madliger

Love

Hultine

et al. 2018

Conservation Physiology

109

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For over a century, physiological tools and techniques have been allowing researchers to characterize how organisms respond to changes in their natural environment and how they interact with human activities or infrastructure. Over time, many of these techniques have become part of the conservation physiology toolbox, which is used to monitor, predict, conserve, and restore plant and animal populations under threat. Here, we provide a summary of the tools that currently comprise the conservation physiology toolbox. By assessing patterns in articles that have been published in ‘Conservation Physiology’ over the past 5 years that focus on introducing, refining and validating tools, we provide an overview of where researchers are placing emphasis in terms of taxa and physiological sub-disciplines. Although there is certainly diversity across the toolbox, metrics of stress physiology (particularly glucocorticoids) and studies focusing on mammals have garnered the greatest attention, with both comprising the majority of publications (>45%). We also summarize the types of validations that are actively being completed, including those related to logistics (sample collection, storage and processing), interpretation of variation in physiological traits and relevance for conservation science. Finally, we provide recommendations for future tool refinement, with suggestions for: (i) improving our understanding of the applicability of glucocorticoid physiology; (ii) linking multiple physiological and non-physiological tools; (iii) establishing a framework for plant conservation physiology; (iv) assessing links between environmental disturbance, physiology and fitness; (v) appreciating opportunities for validations in under-represented taxa; and (vi) emphasizing tool validation as a core component of research programmes. Overall, we are confident that conservation physiology will continue to increase its applicability to more taxa, develop more non-invasive techniques, delineate where limitations exist, and identify the contexts necessary for interpretation in captivity and the wild.

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Calibration of the HemoCue point-of-care analyser for determining haemoglobin concentration in a lizard and a fish

Cited by 16 publications

References 26 publications

Uso de hemoglobinómetro como alternativa para la medición de hemoglobina y hematocrito en muestras de aves

Uso de hemoglobinómetro como alternativa para la medición de hemoglobina y hematocrito en muestras de aves

Hypoxia during incubation does not affect aerobic performance or haematology of Atlantic salmon (Salmo salar) when re-exposed in later life

The conservation physiology toolbox: status and opportunities

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