Chance events such as seed dispersal determine the potential composition of plant communities, but the eventual assemblage is determined in large part by subsequent interactions among species. Postcolonization sorting also affects the ultimate composition of communities assembled by people for restoration, horticulture, or conservation. Thus, knowledge of the mechanisms controlling interspecific interactions in plant communities is important for explaining patterns observed in nature and predicting success or failure of utilitarian combinations. Relationships among species, especially those from studies of biological diversity and ecosystem functioning, are largely based on studies of short-lived, temperatezone plants. Extrapolation to perennial plants in the humid tropics is risky because functional relationships among large-stature species change with time. Shifts in competitive relationships among 3 life forms-trees, palms, and perennial herbs-occurred during 13 yr in experimental tropical ecosystems. In 2 cases the novel competitive mechanism responsible for the shift was reduction in crown volume, and therefore light-capturing capability, of overtopping deciduous trees by intrusive growth from below a palm. In a third case, complementary resource use developed between 2 evergreen life forms (overstory tree and palm), probably because of differential nutrient acquisition. Species-level traits and adequate time for shifts in interspecific relationships to emerge are crucial for predicting community trajectories.complementarity ͉ diversity ͉ ecosystem functioning ͉ fertile soil ͉ plant competition D esign of sustainable ecosystems, whether for biodiversity conservation, economic gain, or restoration of ecosystem services, involves the assembly of plant communities comprising species of high ecological combining ability (1, 2). That combining ability is determined by interspecific interactions and refers to the ways and degree to which common resources, particularly light, water, and mineral nutrients, are shared. Does the nature of plant interactions remain relatively constant over time? If not, prediction of combining ability will require either case-specific long-term observation or thorough understanding of the mechanisms involved.To examine the stability of plant interactions, and to reveal mechanisms driving any changes observed, we constrained the number of perennial life-form groups in simple experimental communities while varying the specific identity of 1 of them. The study site, a young alluvial terrace at La Selva Biological Station in the humid tropical lowlands of Costa Rica, was chosen because of its exceptionally fertile soil and warm, wet climate. By conducting experiments in an environment where plants experienced few abiotic constraints on growth we achieved results quickly and avoided the delays intrinsic to environments where plant growth is slow or where the ecological clock is reset annually.The life forms selected are among those most common in mature forest at La Selva (3) and character...