Calibration, Standardization, and Quantitative Analysis of Multidimensional Fluorescence (Mdf) Measurements on Complex Mixtures

Multi-attribute quality screening of immunoglobulin G using polarized Excitation Emission Matrix spectroscopy

“…The combination of these effects with the environmental-sensitive fluorescence emission, are responsible for the shape observed in the matrix providing a very diagnostic tool for assessing protein changes and stability. EEM can be implemented robustly for routine analytical applications [12], is widely used in water analysis [13] and for cell culture media analysis [14][15][16]. EEM and PARAFAC analysis has also been used to study structure changes in BSA [17].…”

Procedure to build a signal transfer set, independent of the target analytes, between a portable fluorimeter based on light-emitting diodes and a master fluorimeter

“…The IUPAC Technical Report [1] states that the focus of analytical chemistry and spectroscopic analysis is being on the "in situ" measurements which can be easily automated, particularly for the analysis of complex materials for fluorescence. This report considers the recording of excitation-emission matrices (EEM) together with parallel factor analysis (PARAFAC) as a promising tool due to the wide literature on the subject.…”

Derived Absorbance Spectral Parameter as a Tool for Sensitive Fluorescence Measurements of Optically Dense Systems

“…Ryder et al have mentioned that it is better to explicitly include the IFE and to study such natural and biological optically dense systems in integrity. 8 The concomitant effect of exclusive inclusion of IFE in the system can be analyzed and approaches can be developed for facile fluorescence based analysis of optically dense systems. Mishra and co-workers' have demonstrated the use of a derived absorbance spectral parameter (D abs ) in obtaining optimum offset wavelength in synchronous fluorescence spectral (SFS) measurements.…”