Calla lily chlorotic spot virus from Spider Lily (<i>Hymenocallis litteralis</i>) and Tobacco (<i>Nicotiana tabacum</i>) in the South‐west of China

Liu, Yating; Lu, Xiaoxuan; Zhi, L. H.; Zheng, Yanhui; Chen, Xue-Jiao; Xu, Yue; FaJing, Wu; Li, Yongzhong

doi:10.1111/j.1439-0434.2011.01873.x

Cited by 14 publications

(8 citation statements)

References 8 publications

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“…Eleven Tospovirus species have been reported in China (Chen et al, 2007;Ding et al, 2007;Dong et al, 2008Dong et al, , 2010Gu et al, 2012;Liu et al, 2010Liu et al, , 2012Meng et al, 2013;Rao et al, 2013;Xu et al, 2013;Cheng et al, 2014). Of these, tomato zonate spot virus (Dong et al, 2008), HCRV (Xu et al, 2013(Xu et al, , 2014 Mulberry vein banding virus (Meng et al, 2013) and tomato necrotic spot virus (Cheng et al, 2014) were first identified on mainland China.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity of the nucleocapsid protein gene of hippeastrum chlorotic ringspot virus from Hymenocallis littoralis in southern China

LI¹,

Xu²,

Zhu³

et al. 2017

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Mimivirus was the first discovered amoebal giant virus. The Mimivirus virions are covered by a dense layer of approximately 130 nm-long fibers, the length and shape of which diverge from those of other viruses. Here, we aimed at expressing the L725 protein to further confirm and study its role as a fiber-associated protein. We report Escherichia coli expression of the L725 protein, which is encoded by a Mimivirus ORFan, was previously identified by proteomics in purified viral fibers and demonstrated to be a fiber-associated protein by RNA-silencing experiments. The expressed protein was recognized by anti-Mimivirus fiber or anti-Mimivirus L725 polyclonal antibodies. This study is the only expression, to our knowledge, of a product from a Mimiviral ORFan gene.

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Section: Discussionmentioning

confidence: 99%

Genetic diversity of the nucleocapsid protein gene of hippeastrum chlorotic ringspot virus from Hymenocallis littoralis in southern China

LI¹,

Xu²,

Zhu³

et al. 2017

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“…To date, nine members of Tospovirus have infected economically important crops in Yunnan, Guizhou, Guangxi provinces in southwest China. The viruses identified in these regions are: the Calla lily chlorotic spot virus (CCSV) [ 3 ], Capsicum chlorosis virus (CaCV) [ 4 ], Groundnut yellow spot virus (GYSV) [ 5 ], Hippeastrum chlorotic ringspot virus (HCRV) [ 6 ], Impatiens necrotic spot virus (INSV) [ 7 ], Tomato necrotic ring spot virus (TNRSV) [ 8 ], Tomato zonate spot virus (TZSV) [ 9 , 10 ], TSWV [ 11 ] and WSMoV [ 12 ]. Among these tospoviruses, TSWV and TZSV are the dominant species.…”

Section: Introductionmentioning

confidence: 99%

Clustering and cellular distribution characteristics of virus particles of Tomato spotted wilt virus and Tomato zonate spot virus in different plant hosts

Zhang

Zheng²,

Dong³

et al. 2016

Virol J

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BackgroundTomato spotted wilt virus (TSWV) and Tomato zonate spot virus (TZSV) are the two dominant species of thrip-transmitted tospoviruses, cause significant losses in crop yield in Yunnan and its neighboring provinces in China. TSWV and TZSV belong to different serogroup of tospoviruses but induce similar symptoms in the same host plant species, which makes diagnostic difficult. We used different electron microscopy preparing methods to investigate clustering and cellular distribution of TSWV and TZSV in the host plant species.ResultsNegative staining of samples infected with TSWV and TZSV revealed that particles usually clustered in the vesicles, including single particle (SP), double particles clustering (DPC), triple particles clustering (TPC). In the immunogold labeling negative staining against proteins of TZSV, the antibodies against Gn protein were stained more strongly than the N protein. Ultrathin section and high pressure freeze (HPF)-electron microscopy preparations revealed that TSWV particles were distributed in the cisternae of endoplasmic reticulum (ER), filamentous inclusions (FI) and Golgi bodies in the mesophyll cells. The TSWV particles clustered as multiple particles clustering (MPC) and distributed in globular viroplasm or cisternae of ER in the top leaf cell. TZSV particles were distributed more abundantly in the swollen membrane of ER in the mesophyll cell than those in the phloem parenchyma cells and were not observed in the top leaf cell. However, TZSV virions were mainly present as single particle in the cytoplasm, with few clustering as MPC.ConclusionIn this study, we identified TSWV and TZSV particles had the distinct cellular distribution patterns in the cytoplasm from different tissues and host plants. This is the first report of specific clustering characteristics of tospoviruses particles as well as the cellular distribution of TSWV particles in the FI and globular viroplasm where as TZSV particles inside the membrane of ER. These results indicated that tospoviruses particles possessed specific and similar clustering in the saps of diseased plants. Furthermore, the results of this study will also provide a basis for further study on the tospoviruses assembling, maturation and movement.

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“…TZSV currently becomes the important threat infecting tomato, tobacco and ornamentals in southwestern China, and Frankliniella occidentalis (Pergande) is its main transmissible vector [ 18 , 20 – 22 ]. Calla lily chlorotic spot virus (CCSV), first collected from calla lily in Taiwan, is occurring in Yunnan Province that infects tobacco and spider lily [ 23 , 24 ]. The transmissible vector of CCSV and TNSaV in China remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in Southwestern China

Chen

Dong²,

Chien

et al. 2016

Virol J

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BackgroundThe thrips-borne tospoviruses Calla lily chlorotic spot virus (CCSV), Tomato zonate spot virus (TZSV) and a new species provisionally named Tomato necrotic spot associated virus (TNSaV) infect similar crops in southwestern China. The symptoms exhibiting on virus-infected crops are similar, which is difficult for distinguishing virus species by symptomatology. The sequences of nucleocapsid proteins (NPs) of CCSV, TNSaV and TZSV share high degrees of amino acid identity with each other, and their serological relationship was currently demonstrated from the responses of the previously reported monoclonal antibodies (MAbs) against the NP of CCSV (MAb-CCSV-NP) and the nonstructural NSs protein of Watermelon silver mottle virus (WSMoV) (MAb-WNSs). Therefore, the production of virus-specific antibodies for identification of CCSV, TNSaV and TZSV is demanded to improve field surveys.MethodsThe NP of TZSV-13YV639 isolated from Crinum asiaticum in Yunnan Province, China was bacterially expressed and purified for producing MAbs. Indirect enzyme-linked immunosorbent assay (ELISA) and immunoblotting were conducted to test the serological response of MAbs to 18 tospovirus species. Additionally, the virus-specific primers were designed to verify the identity of CCSV, TNSaV and TZSV in one-step reverse transcription-polymerase chain reaction (RT-PCR).ResultsTwo MAbs, denoted MAb-TZSV-NP(S15) and MAb-TZSV-NP(S18), were screened for test. MAb-TZSV-NP(S15) reacted with CCSV and TZSV while MAb-TZSV-NP(S18) reacted specifically to TZSV in both indirect ELISA and immunoblotting. Both MAbs can be used to detect TZSV in field-collected plant samples. The epitope of MAb-TZSV-NP(S18) was further identified consisting of amino acids 78–86 (HKIVASGAD) of the TZSV-13YV639 NP that is a highly conserved region among known TZSV isolates but is distinct from TNSaV and TZSV.ConclusionsIn this study, two MAbs targeting to different portions of the TZSV NP were obtained. Unlike MAb-CCSV-NP reacted with TNSaV as well as CCSV and TZSV, both TZSV MAbs can be used to differentiate CCSV, TNSaV and TZSV. The identity of CCSV, TNSaV and TZSV was proven by individual virus-specific primer pairs to indicate the correctness of serological responses. We also proposed an serological detection platform using MAb-CCSV-NP, MAb-TZSV-NP(S15) and MAb-TZSV-NP(S18) to allow researchers and quarantine staff to efficiently diagnose the infections of CCSV, TNSaV and TZSV in China and other countries.Electronic supplementary materialThe online version of this article (doi:10.1186/s12985-016-0525-3) contains supplementary material, which is available to authorized users.

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Calla lily chlorotic spot virus from Spider Lily (Hymenocallis litteralis) and Tobacco (Nicotiana tabacum) in the South‐west of China

Cited by 14 publications

References 8 publications

Genetic diversity of the nucleocapsid protein gene of hippeastrum chlorotic ringspot virus from Hymenocallis littoralis in southern China

Genetic diversity of the nucleocapsid protein gene of hippeastrum chlorotic ringspot virus from Hymenocallis littoralis in southern China

Clustering and cellular distribution characteristics of virus particles of Tomato spotted wilt virus and Tomato zonate spot virus in different plant hosts

Monoclonal antibodies for differentiating infections of three serological-related tospoviruses prevalent in Southwestern China

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