Callogénesis in vitro de durazno (Prunus persica L.) var. Huayco rojo a partir de explantes foliares

Amasifuen, Angel David Hernández; Cortez-Lázaro, Anthony Apolinario; Curaca, Alexis Argüelles; Pillasca, Hermila Belba Díaz

doi:10.21930/rcta.vol23_num1_art:2032

Cited by 7 publications

(4 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Similarly, the results obtained were superior to those obtained by Koleva et al 27 The growth regulator 2,4-D is added to the culture media to induce the proliferation of undifferentiated cells, forming the characteristic cell mass called callus 28 . The 2,4-D has a rapid induction effect initially at the edges of the initial explant until it covers the entire structure, depending on the concentrations, combinations with other growth regulators, initial explant and the species with which the research is being carried out [29][30][31] . From this embryogenic callus, it was possible to observe the formation of somatic embryos after 12 weeks in the culture medium, concerning the fact that some species have the characteristic of the formation of somatic embryos after the formation of callus (indirect somatic embryogenesis), and likewise, remaining in the same culture medium without changing it or refreshing the medium 32 .…”

Section: Discussionmentioning

confidence: 99%

The efficient procedure of embryogenic callus formation from anther in Capsicum pubescens Ruiz & Pav

2023

View full text Add to dashboard Cite

The peppers or ajies (Capsicum spp.) are one of Peru's main productive crops, which is why it is among the top ten countries in production and exports. Among the species cultivated in Peru is Capsicum pubescens Ruiz & Pav., commonly called rocoto, and represents one of the most critical species in local and national gastronomy. The rocoto ecotype "Selva" or "de Monte" is characterized by its large size and is used to prepare rocoto relleno arequipeño. This crop presents the restriction to obtain homozygous lines for being self-incompatible. Using biotechnology, pure lines can be obtained from the anther culture, and homozygous lines can be obtained. The objective was to induce embryogenic callus formation from anther culture of Capsicum pubescens Ruiz & Pav. Ecotype "de Monte". In the results, 74.1% of embryogenic callus was induced in rocoto anthers with a longitudinal ratio of 1.04 mm; the induction was carried out using Murashige and Skoog culture medium added with 2.0 mg/L of 2,4-D. Likewise, the formation of somatic embryos in the globular stage was evidenced after 12 weeks in the culture medium.

show abstract

Section: Discussionmentioning

confidence: 99%

The efficient procedure of embryogenic callus formation from anther in Capsicum pubescens Ruiz & Pav

2023

View full text Add to dashboard Cite

show abstract

“…A balance between auxins and cytokinins leads to callus development [61]. However, multiple research works have reported that the use of 2,4-D induces in vitro callus formation from various explants, positioning it as one of the regulators that better promotes the callus formation, whether or not it is combined with cytokinins [62][63][64][65].…”

Section: Experimental Phase 2: Callus Formation Protocolmentioning

confidence: 99%

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

de la Luz,

Román,

Jorge

et al. 2024

Horticulturae

View full text Add to dashboard Cite

Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the shoot propagation, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL−1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds and shoots and the shoot height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L−1 of BA and 0.1 mg L−1 of TDZ, as well as the MS base culture medium. The validation of the results of the first phase (0.1 mg L−1 of BA) was compared with the MS in an independent experiment against the control (n = 50 repetitions), obtaining a height of 52 mm, 1.36 shoots, and 9.22 buds, suggesting that this concentration is adequate for the purpose, surpassing the MS control (MS culture medium alone). Of the total volume of roots obtained with packed bud structure in the previous experimental sample, it was reduced to 14% (n = 50). The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L−1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. Callus induction in S. compositum was better when using the stem in a medium with 2.0 mg L−1 of 2,4-D with a value of 97.8% around the explant. The addition of 500 mg L−1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callus formation of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

show abstract

“…A balance between auxins and cytokinins leads to callus development [54]. However, multiple research works have reported that the use of 2,4-Dichlorophenoxyacetic acid (2,4-D) induces in vitro callus formation from various explants, positioning it as one of the regulators that better promotes callogenesis, whether, or not it is combined with cytokinins [55][56][57][58].…”

Section: Experimental Phase 2: Callogenesis Inductionmentioning

confidence: 99%

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Luz,

Román,

Mar

et al. 2023

Preprint

View full text Add to dashboard Cite

Sechium compositum (Cucurbitaceae) is a wild species that is distributed in the Soconusco region, Chiapas, Mexico, and the border with Guatemala. This species has an intangible biochemical value resulting from the pharmacological relevance of its secondary metabolites. However, as a consequence of the lack of knowledge about its importance, it is being displaced from its habitat at an accelerated rate, incurring the risk of genetic loss. Therefore, an in vitro culture protocol with two experimental phases was evaluated to propagate, conserve, and regenerate this species. The first phases considered the multiplication of seedlings, adding seven concentrations (0.1, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 mg mL-1) of 6-benzylaminopurine (BA) and thidiazuron (TDZ) and evaluating the number of buds, shoots, and seedling height. The best multiplication response was recorded with 0.1, 0.2, 0.4, and 1.0 mg L-1 of BA and 0.1 mg L-1 of TDZ, as well as the MS base culture medium. With 0.1 mg L-1 of BA, 52-mm height, 1.36 shoots, and 9.22 buds were obtained, surpassing the MS control (MS culture medium alone); likewise, twice more roots (80%) and a 14% reduction in the bud structure were packed in 50 repetitions. The second phase consisted of inducing callus formation from stem and leaf explants through the addition of 0.5, 1.0, and 2.0 mg L-1 of TDZ and 2,4-Dichlorophenoxyacetic acid (2,4-D) to the medium. The results demonstrated that the induction of callogenesis in S. compositum has a better start from the stem in a medium with 2.0 mg L-1 of 2,4-D, whose value was 4.89 —equivalent to 97.8% callus formation around the explant. The addition of 500 mg L-1 of polyvinylpyrrolidone (PVP) is also suggested to reduce oxidation. This protocol represents a significant advance in the conservation, multiplication, and callogenesis of S. compositum and contributes to its rescue and revaluation in the face of the danger of extinction.

show abstract

Callogénesis in vitro de durazno (Prunus persica L.) var. Huayco rojo a partir de explantes foliares

Cited by 7 publications

References 17 publications

The efficient procedure of embryogenic callus formation from anther in Capsicum pubescens Ruiz & Pav

The efficient procedure of embryogenic callus formation from anther in Capsicum pubescens Ruiz & Pav

In Vitro Shoot Regeneration and Callogenesis of Sechium compositum (Donn. Sm.) C. Jeffrey for Plant Conservation and Secondary Metabolites Product

Bud Multiplication and In Vitro Callogenesis of Sechium compositum (Donn. sm.) C. Jeffrey from Stem and Leaf Explants

Contact Info

Product

Resources

About