Callose synthesis in higher plants

Chen, Xiong-Yan; Kim, Jae‐Yean

doi:10.4161/psb.4.6.8359

Cited by 393 publications

(283 citation statements)

References 26 publications

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“…To test this, we performed immunofluorescence experiments using three representative cell wall antibodies against callose, (1,3;1,4)-b-glucan, and pectin. In developing wild-type subaleurone cells, callose was deposited as dotted structures at the plasmodesmata (Supplemental Figure 6A), as reported previously (Chen and Kim, 2009). In contrast, (1,3;1,4)-b-glucan and pectin were deposited evenly in the cell wall (Supplemental Figures 6C and 6E).…”

Section: Abnormal Deposition Of Callose and Cell Wall Components In Pmbssupporting

confidence: 57%

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GLUTELIN PRECURSOR ACCUMULATION3Encodes a Regulator of Post-Golgi Vesicular Traffic Essential for Vacuolar Protein Sorting in Rice Endosperm

et al. 2014

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In seed plants, a major pathway for sorting of storage proteins to the protein storage vacuole (PSV) depends on the Golgi-derived dense vesicles (DVs). However, the molecular mechanisms regulating the directional trafficking of DVs to PSVs remain largely elusive. Here, we report the functional characterization of the rice (Oryza sativa) glutelin precursor accumulation3 (gpa3) mutant, which exhibits a floury endosperm phenotype and accumulates excess proglutelins in dry seeds. Cytological and immunocytochemistry studies revealed that in the gpa3 mutant, numerous proglutelin-containing DVs are misrouted to the plasma membrane and, via membrane fusion, release their contents into the apoplast to form a new structure named the paramural body. Positional cloning of GPA3 revealed that it encodes a plant-specific kelch-repeat protein that is localized to the trans-Golgi networks, DVs, and PSVs in the developing endosperm. In vitro and in vivo experiments verified that GPA3 directly interacts with the rice Rab5a-guanine exchange factor VPS9a and forms a regulatory complex with Rab5a via VPS9a. Furthermore, our genetic data support the notion that GPA3 acts synergistically with Rab5a and VPS9a to regulate DV-mediated post-Golgi traffic in rice. Our findings provide insights into the molecular mechanisms regulating the plant-specific PSV pathway and expand our knowledge of vesicular trafficking in eukaryotes.

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Section: Abnormal Deposition Of Callose and Cell Wall Components In Pmbssupporting

confidence: 57%

“…It is known that callose synthesis and deposition can be quickly induced by biotic stresses such as plasmolysis in plants (Chen and Kim, 2009). The uploading of mistargeted DV cargos into the apoplast in the gpa3 mutant may represent a physiologically similar process to plasmolysis.…”

Section: Mistargeting Of Dvs To the Pm Leads To Pmb Formationmentioning

confidence: 99%

GLUTELIN PRECURSOR ACCUMULATION3Encodes a Regulator of Post-Golgi Vesicular Traffic Essential for Vacuolar Protein Sorting in Rice Endosperm

et al. 2014

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“…The first subgroup of AtGSLs includes AtGSL1, AtGSL5, AtGSL8, and AtGSL10; the second subgroup contains AtGSL2, AtGSL3, AtGSL6, and AtGSL12; the third subgroup consists of AtGSL7 and AtGSL11; and the fourth subgroup consists of AtGSL4. 12 Most of the GSLs in Arabidopsis have been cloned, and the functions of the corresponding GSLs have been identified. The number of similar studies in rice is limited， only GSL5 has been reported so far (Fig.…”

Section: Gsls In Monocotyledonous and Dicotyledonous Plantsmentioning

confidence: 99%

Callose synthesis during reproductive development in monocotyledonous and dicotyledonous plants

Xiao

Han

2015

Plant Signaling & Behavior

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Callose, a linear b-1,3-glucan molecule, plays important roles in a variety of processes in angiosperms, including development and the response to biotic and abiotic stress. Despite the importance of callose deposition, our understanding of the roles of callose in rice reproductive development and the regulation of callose biosynthesis is limited. GLUCAN SYNTHASE-LIKE genes encode callose synthases (GSLs), which function in the production of callose at diverse sites in plants. Studies have shown that callose participated in plant reproductive development, and that the timely deposition and degradation of callose were essential for normal male gametophyte development. In this mini-review, we described conserved sequences found in GSL family proteins from monocotyledonous (Oryza sativa and Zea mays) and dicotyledonous (Arabidopsis thaliana and Glycine max) plants. We also describe the latest findings on callose biosynthesis and deposition during reproductive development and discuss future challenges in unraveling the mechanism of callose synthesis and deposition in higher plants.

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“…Callose is a natural component of plant cell wall and it is abundant in pollen tubes (Cresti & van Went, 1976). In some situation, its formation can be regarded as a stress response (Chen & Kim, 2009). Our reported fiber structure of callose is unique.…”

Section: Introductionmentioning

confidence: 99%

Development of a method to measure mechanical properties of single elongated callose fibers in protoplast cultures of Larix leptolepis and Betula platyphylla

Oyanagi

Kurita

Shiraishi

et al. 2016

JPS

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We developed a method to measure mechanical properties of single fibers of callose in liquid protoplast cultures of Larix leptolepis and Betula platyphylla, which were formed in media containing 50 mM of MgCl 2 or 100 mM of CaCl 2 , respectively. Tensile test was performed using two micromanipulators loading micropipettes under an inverted microscope. Spring constant of the pipette used was first calibrated and calculated from using a microbalance. The callose fiber was wired between the two micropipettes. The Young's modulus of single fibers for Larix and Betula was 7-9 kPa (1.4-1.9 x 10 4 N/m 2 ) though the diameters of the fiber varied from 10 μm for Larix and 22-26 μm for Betula. No big difference was found between experiments with and without medium containing high concentrations of salts. Tensile strength at break was 1.1-1.8 kPa (2.3-3.6 x 10 3 N/m 2 ). The values are compared to other materials including cellulose containing plant cell wall, cell membranes, and amorphous callose. The value of the Young's modulus observed was discussed.

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Callose synthesis in higher plants

Cited by 393 publications

References 26 publications

GLUTELIN PRECURSOR ACCUMULATION3Encodes a Regulator of Post-Golgi Vesicular Traffic Essential for Vacuolar Protein Sorting in Rice Endosperm

GLUTELIN PRECURSOR ACCUMULATION3Encodes a Regulator of Post-Golgi Vesicular Traffic Essential for Vacuolar Protein Sorting in Rice Endosperm

Callose synthesis during reproductive development in monocotyledonous and dicotyledonous plants

Development of a method to measure mechanical properties of single elongated callose fibers in protoplast cultures of Larix leptolepis and Betula platyphylla

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